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ProteomicsMicrowave oven-assisted derivatization of histone proteoforms using trimethylacetic anhydride
We introduce a new reagent – Trimethylacetic anhydride (TMA) for highly efficient histone derivatization, which is facilitated by microwave irradiation to reduce sample preparation duration. The derivatization of unmodified NH2- groups at histone lysine residues is necessary prior LC-MS analysis usi...
ORGANISM(S): Homo sapiens (Human) 
2021-06-14 | PXD019502 | Pride
O histone derivatization Epigenetics Mass spectrometry Trimethylacetic anhydride 
ProteomicsArg-C Ultra simplifies histone preparation for LC-MS/MS
Using mammalian histone extract, we demonstrate that Arg-C Ultra facilitates histone preparation for LC-MS/MS. We show the performance of Arg-C Ultra in terms of digestion specificity, number of modified forms identified, and yield of quantitative information compared with standard Arg-C and trypsin...
ORGANISM(S): Homo sapiens (Human) 
2025-06-30 | PXD059514 | Pride
Histone Arg-c ultra Trimethylacetic anhydride Lc-ms/ms Chemical derivatization 
UnknownTrimethylacetic Anhydride-Based Derivatization Facilitates Quantification of Histone Marks at the MS1 Level.
Not available
S-EPMC8283018 | biostudies-literature
ProteomicsMicrowave oven-assisted derivatization of histone proteoforms using trimethylacetic anhydride
Histone post-translational modifications (hPTMs) belong to decisive epigenetic marks affecting, among others, cell cycle and protein interactions. hPTMs have been studied by both antibody- and mass spectrometry-based methods for years, however, those analyses are still challenging, mainly due to mul...
ORGANISM(S): Homo Sapiens 
2021-06-15 | PXD022454 | panorama
ProteomicsEpigenetic and transcriptional control of adipocyte function by centenarian-associated SIRT6 N308K/A313S mutant
Obesity is a major health burden. Adipogenesis, the proliferation and differentiation of pree-adipocytes in mature adipocytes, could be a potential therapeutic approach for obesity. Deficiency of SIRT6, a member of the sirtuin family of nicotinamide adenine dinucleotide (NAD)+-dependent protein deac...
ORGANISM(S): Homo sapiens (Human) 
2024-08-10 | PXD051761 | Pride
Adipogenesis Epigenetics Obesity. Histones Sirt6 
UnknownArg-C Ultra Simplifies Histone Preparation for LC-MS/MS.
Not available
S-EPMC12199229 | biostudies-literature
ProteomicsDissection of acetylomic profiles in T cell lymphoma following histone deacetylases inhibition
The aim was to dissect molecular changes in histone and non-histone protein acetylation dynamics following the genetic or pharmacological inhibition of HDAC activity in a model of Anaplastic Large Cell Lymphoma (ALCL), a T cell non-Hodgkin lymphoma, predominantly found in children and adolescents.
ORGANISM(S): Mus musculus (Mouse) 
2022-10-14 | PXD030503 | Pride
Acetylomics Histone deacetylase inhibitors Anaplastic large cell lymphoma Histone deacetylases Proteomics 
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