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ProteomicsTruncation of the N-terminus of cardiac troponin I initiates adaptive remodeling of the myocardial proteosome via phosphorylation of mechano-sensitive signaling pathways
We employed isobaric labeled peptides to do bottom-up proteomics to quantify both non-enriched total peptides and enriched phospho-peptides obtained from hearts of control mice expressing wild-type cardiac troponin I and transgenic mice expressing the truncated N-terminal cardiac troponin I.
ORGANISM(S): Mus Musculus (ncbitaxon:10090) 
2021-03-15 | MSV000087049 | MassIVE
ProteomicsParallel genetic excisions of the cardiac troponin I N-terminal extension in tachycardic mammals
A study of cardiac troponin I evolution in mammals with high heart rates, including shrews, moles, and bats. With genomic, mRNA and protein level analyses we showed repeated loss of the N-terminal extension. Here, liquid chromatography with tandem mass spectrometry was used to verify cardiac troponi...
ORGANISM(S): Galemys pyrenaicus Blarina brevicauda 
2024-07-19 | PXD048600 | Pride
Troponin Sarcomere Shrew Mole 
TranscriptomicsTranscription profiling of zebrafish embryos following cardiac troponin T2 knockdown
We examined the transcriptional effect of preventing cardiac contraction in zebrafish embryos which can be deprived of circulation without experiencing hypoxia since the fish obtain sufficient oxygen via diffusion. Morpholino antisense knockdown of cardiac troponin T2 (tnnt2) prevented cardiac contr...
ORGANISM(S): Danio rerio 
2009-07-05 | E-GEOD-16740 | biostudies-arrayexpress
ProteomicsIntact Transition Epitope Mapping - Force differences between Original and Unusual Residues (ITEM-FOUR)
Human cardiac Troponin I epitope peptides were incubated with monoclonal anti-cardiac Troponin I antibody. Immune complexes were subjected to nano electrospray mass spectrometry in order to determine apparent binding energies and dissociation constants of immune complex dissociation reactions in the...
ORGANISM(S): Homo sapiens (Human) 
2023-01-17 | PXD038365 | Pride
Item-four Troponin i Offline nanoesi-ms 
ProteomicsMass Spectrometric Analysis of Antibody-Epitope Peptide Complex Dissociation: Theoretical Concept and Practical Procedure of Binding Strength Characterization
We describe theoretical concepts and explain practical procedures of an electrospray mass spectrometry method, termed “Intact Transmission Epitope Mapping – Thermodynamic Weak-force Order (ITEM-TWO)”, by which can be determined apparent binding energies and dissociation constants of immune complex ...
ORGANISM(S): Homo sapiens (Human) Equus caballus (Horse) 
2020-10-29 | PXD021296 | Pride
Protocol Item-two Rnase s Offline esi-ms Troponin i epitope peptide-antitroponin i antibody complex Myoglobin Flag-peptide–antiflag antibody complex 
ProteomicsQuantification of Cardiac Troponin I Using ID-LC-MS/MSQuantification of Cardiac Troponin I Using ID-LC-MS/MS
An analytical protocol based on isotope dilution liquid chromatography-tandem mass spectrometry (ID-LC-MS/MS) which includes a peptide-based calibration strategy was developed and validated for accurate determination of cardiac troponin I (cTnI) levels in clinical samples.
ORGANISM(S): Homo sapiens (Human) 
2025-04-08 | PXD055104 | Pride
Picaa Immunoaffinity enrichment Peptide-based calibration Id-ms Ctni Magnetic particles 
TranscriptomicsTranscription profiling of wild type wild type ,TnT transgenic for human Troponin ( non mutated), T10 transgenic for human truncated Troponin
WT wild type ,TnT transgenic for human Troponin ( non mutated), T10 transgenic for human truncated Troponin
ORGANISM(S): Rattus norvegicus 
2003-05-22 | E-RZPD-6 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of wild type and delK210 cTnT knock-in mouse heart to study dilated cardiomyopathy
Changes in the gene expression in the heart of knock-in mouse model of dilated cardiomyopathy caused by delK210 mutation in cardiac troponin T.
ORGANISM(S): Mus musculus 
2008-09-04 | E-MEXP-1340 | biostudies-arrayexpress
UnknownDetection of a novel sense-antisense RNA-hybrid structure by RACE experiments on endogenous troponin I antisense RNA.
Not available
S-EPMC1370611 | biostudies-literature
TranscriptomicsCardiac transcription factors in HL-1 cells: gene expression and genome binding profiling
[1] Gene expression profiling in Gata4, Mef2a knockdown in HL-1 cells. HL-1 cells infected with adenovirus expressing either control siRNA or Gata4, and Gata4 & Mef2a siRNA. [2] Genome-wide maps of cardiac transcription factors binding region in HL-1 cells. We performed bio-ChIP-seq using streptavi...
ORGANISM(S): Mus musculus 
2011-03-02 | E-GEOD-21529 | biostudies-arrayexpress
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