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ProteomicsReconstitution of 3’-processing of mammalian pre-mRNA reveals a central role of RBBP6
The 3’ ends of almost all eukaryotic mRNAs are generated in an essential two-step processing reaction, endonucleolytic cleavage of an extended precursor followed by the addition of a poly(A) tail. By reconstituting the reaction from overproduced and purified proteins, we provide a minimal list of fo...
ORGANISM(S): Homo sapiens (Human) 
2022-03-04 | PXD031227 | Pride
Cpsf 3’-processing Xl-ms Rbbp6 
ProteomicsTranslational repression of the Drosophila nanos mRNA involves the RNA helicase Belle and RNA coating by Me31B and Trailer hitch
The maternal mRNA nanos is required for anterior-posterior patterning in the early Drosophila melanogaster development. The nanos-mRNA is translationally repressed and deadenylated during the first two hours of embryogenesis. A stem-loop structure in the 3'-UTR of this RNA (SRE - Smaug recogniiton e...
ORGANISM(S): Drosophila melanogaster (Fruit fly) 
2017-07-18 | PXD006596 | Pride
Cup Ccr4-not-complex Deadenylation dead-box helicase Trailer hitch Nanos Me31b Translational repression Smaug 
ProteomicsReconstitution of mammalian Cleavage Factor II
Cleavage factor II (CF II) is a poorly characterized component of the multi-protein complex catalyzing 3' cleavage and polyadenylation of mammalian mRNA precursors. We have reconstituted CF II as a heterodimer of hPcf11 and hClp1. The heterodimer is active in partially reconstituted cleavage reactio...
ORGANISM(S): Homo sapiens (Human) 
2020-05-26 | PXD010177 | Pride
Human Rna processing Dsbu 
ProteomicsPrecise temporal regulation of post-transcriptional repressors is required for an orderly Drosophila maternal-to-zygotic transition
In animal embryos the maternal-to-zygotic transition (MZT) hands developmental control from maternal to zygotic gene products. We show that the maternal proteome represents over half of the protein coding capacity of the Drosophila melanogaster genome and that 2% of this proteome is rapidly degraded...
ORGANISM(S): Drosophila melanogaster (Fruit fly) 
2021-09-09 | PXD018794 | Pride
C-terminal to lis1 homology (ctlh) complex Skp/cullin/f-box-containing (scf) complex Ubiquitin ligase Smaug Rna-binding protein 
Transcriptomicspoly(A) specific ribonuclease (PARN)-dependent trimming of H ACA box RNAs
Microarray comprising probe sets for miRNAs and other small RNAs was used to determine which and how many small RNAs are potential substrates of poly(A) specific ribonuclease (PARN) in U2OS cells . Hybridization probes were generated by oligo(dT) priming.
ORGANISM(S): Homo sapiens 
2012-02-17 | E-MEXP-3344 | biostudies-arrayexpress
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