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ProteomicsA PrSM-level Side-by-Side Comparison of On-line LCMS methods on Intact Histone H3 and H4 Proteoforms
The heterogeneity of histone H3 proteoforms has made histone H3 top-down analysis challenging. To enhance the detection coverage on the proteoforms, performing liquid chromatography (LC) front-end to MS detection is recommended. Here, by using optimized Electron-Transfer/High-Energy Collision Dissoc...
ORGANISM(S): Homo sapiens (Human) 
2025-03-03 | PXD026820 | Pride
Top-down proteomics Histones Post-translation modifications On-line lcms Rplc Esi-compatible wcx/hilic 
ProteomicsOnline Weak Cation Exchange-HILIC × Reversed Phase LC UVPD-HRMS of intact HeLa Histones
Intact HeLa core Histones were analyzed using an online two-dimensional liquid chromatographic separation as well as one dimensional LC (RPLC and WCX-HILIC) coupled with UVPD-MS
ORGANISM(S): Homo sapiens (Human) 
2018-09-19 | PXD008978 | Pride
Histones Top-down proteomics Online 2dlc Uvpd 
ProteomicsOn-line desalting of sodium dodecyl sulfate in mass spectrometry
We tested here the ability of weak cation exchange (WCX) stationary phase to retain positively charged proteinaceous whilst negatively charged SDS molecules can be washed out from the capillary during on-line desalting. This novel chromatographic method named as online desalting of sodium dodecyl s...
ORGANISM(S): Homo sapiens (Human) 
2018-06-18 | PXD006035 | Pride
Sodium dodecyl sulfate Wcx Mass spectrometry Weak cation exchange Sds Lc-ms/ms 
UnknownStrong metal-support interaction of Ru-NPs/WC<sub>x</sub> boosts alkaline hydrogen evolution and oxidation for alkaline fuel cells and electrolyzers.
Not available
S-EPMC12660761 | biostudies-literature
UnknownImpact of linker-drug on ion exchange chromatography separation of antibody-drug conjugates.
Not available
S-EPMC6748606 | biostudies-literature
ProteomicsMiddle-Down Proteomic Analyses with Ion Mobility Separations of Endogenous Isomeric Proteoforms
We integrated FAIMS into the WCX-HILIC ETD MS/MS system to improve identification of histonetail proteforms
ORGANISM(S): Mus musculus (Mouse) 
2020-04-02 | PXD016918 | Pride
Faims Histones Etd Middle down 
GenomicsssDNA is an allosteric regulator of the C. crescentus SOS-independent DNA damage response transcription activator, DriD
DNA damage repair systems are critical for genomic integrity. However, they must be coordinated with DNA replication and cell division to ensure accurate genomic transmission. In most bacteria this coordination is mediated by the SOS response through LexA , which triggers a halt in cell division unt...
ORGANISM(S): Caulobacter vibrioides 
2022-05-15 | GSE197978 | GEO
UnknownImproved classification of breast cancer peptide and protein profiles by combining two serum workup procedures.
Not available
S-EPMC3491194 | biostudies-literature
UnknownStructure and functional implications of WYL domain-containing bacterial DNA damage response regulator PafBC.
Not available
S-EPMC6789036 | biostudies-literature
UnknownMesoporous WCx Films with NiO-Protected Surface: Highly Active Electrocatalysts for the Alkaline Oxygen Evolution Reaction.
Not available
S-EPMC8596595 | biostudies-literature
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