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ProteomicsDiscovery of arginine methylation, phosphorylation and their co-occurrence in condensate-associated proteins in Saccharomyces cerevisiae
The formation of condensates in membraneless organelles is thought to be driven by protein phase separation. Arginine methylation and serine/threonine phosphorylation are important in the phase separation process, however these post-translational modifications are often present in intrinsically diso...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2021-09-17 | PXD022403 | Pride
Yeast Methylation Phosphorylation Crosstalk 
ProteomicsCharacterisation of protein methyltransferases Rkm1, Rkm4, Efm4, Efm7, Set5 and Hmt1 reveals extensive post-translational modification
Protein methylation is one of the major post-translational modifications (PTMs) in the cell. In Saccharomyces cerevisiae, over twenty protein methyltransferases (MTases) and their respective substrates have been identified. However, the way in which these MTases are modified, and potentially subject...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2017-12-18 | PXD006767 | Pride
Metyltransferases Post-translational modifications Rkm1 Hmt1 Efm7 Rkm4 Set5 Efm4 
ProteomicsMT-MAMS: protein methyltransferase motif analysis by mass spectrometry
Protein methyltransferases can recognise their substrates through linear sequence motifs and determination of these motifs is critical to understand methyltransferase mechanism, function and drug targeting. Here we describe MT-MAMS (methyltransferase motif analysis by mass spectrometry), a quantitat...
ORGANISM(S): Homo sapiens (Human) Saccharomyces cerevisiae (Baker's yeast) 
2018-09-03 | PXD009515 | Pride
Motif Methylation Methyltransferase 
ProteomicsH3K79 methyltransferase Dot1p/DOT1L is phosphorylated by proline-directed MAP kinases Hog1p and p38/MAPK11
Disruptor of telomeric silencing (Dot1p) is an exquisitely conserved histone methyltransferase, and is the sole enzyme responsible for H3K79 methylation in the budding yeast, Saccharomyces cerevisiae. It has been shown to be highly phosphorylated in vivo; however, the upstream kinases that act on Do...
ORGANISM(S): Homo sapiens (Human) Saccharomyces cerevisiae (Baker's yeast) 
2024-01-29 | PXD036747 | Pride
Yeast Signalling Post-translational regulation Histone methylation Epigenetics Chromatin 
ProteomicsMETTL21B is a novel human lysine methyltransferase of translation elongation factor 1A: discovery by CRISPR/Cas9 knock out
Lysine methylation is widespread on human proteins, however the enzymes that catalyse its addition remain largely unknown. This limits our capacity to study the function and regulation of this modification. Here we report that human METTL21B is a protein methyltransferase, which methylates lysine 16...
ORGANISM(S): Homo sapiens (Human) 
2017-07-03 | PXD005497 | Pride
Methyltransferase Cas9 Knockout Crispr Methylation 
ProteomicsNovel roles for ribosomal histidine methylation revealed by proteomics and large-scale cross-linking mass spectrometry
The methylation of histidine residues is increasingly found to be both prevalent throughout the proteome, and also relevant to human disease. Hpm1p mono-methylates H243 in the ribosomal protein Rpl3 and represents the only histidine methyltransferase in Saccharomyces cerevisiae. Interestingly, the h...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2022-08-12 | PXD027953 | Pride
Cross-linking Yeast Silac Histidine methylation 
ProteomicsComprehensive identification of crosslinked peptides from methyltransferase Hmt1 and its substrate Npl3: use of multiple crosslinkers, mass spectrometry approaches and software platforms
Chemical-Crosslinking Mass Spectrometry (XLMS) using MS-cleavable crosslinkers is fast becoming an established technique in the study of protein-protein interactions for both small and large scale samples. With the increased uptake of XLMS as a technique, different combinations of crosslinker types,...
ORGANISM(S): Escherichia coli Saccharomyces cerevisiae (Baker's yeast) 
2018-07-20 | PXD008348 | Pride
Crosslinking-mass spectrometry Ms-cleavable linkers 
ProteomicsYeast methylarginine peptide IPS
Yeast peptides immunoprecipitated with an anti-mono-methylarginine antibody, analysis on Orbitrap Velos with ETD.
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2017-07-03 | PXD002151 | Pride
Lc-msms Arginine Yeast Methylation 
ProteomicsYeast Nuclear Large Scale Cross-linking MS
Here, we apply large scale cross-linking mass spectrometry to profile the interactome of isolated and intact Saccharomyces cerevisiae nuclei. Two independent biological replicates (nuclei isolated from different cultures) were performed.
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2020-03-12 | PXD014584 | Pride
Yeast Dsso Xl-ms Nucleus Crosslinking 
ProteomicsPost-translational modification profiling of Saccharomyces cerevisiae histone methylation enzymes uncovers regulation by upstream signalling pathways
Histone methylation is central to the regulation of eukaryotic transcription. In Saccharomyces cerevisiae, it is controlled by a system of four methyltransferases (Set1p, Set2p, Set5p, and Dot1p) and four demethylases (Jhd1p, Jhd2p, Rph1p, and Gis1p). While the histone targets for these enzymes are ...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2020-12-15 | PXD021214 | Pride
Histone methylation Yeast Post-translational modification Epigenetics Kinase Phosphorylation 
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