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ProteomicsACBD6-related progressive neurodevelopmental disorder – whole proteome of developing Danio rerio (zebrafish)
In triplicate for each condition, 12 WT and acbd6 F0 crispant Danio rerio (zebrafish) embryos were incubated with 20 μM YnMyr for 24 h, either between 48-72 hpf or 96-120 hpf. After labelling, zebrafish were washed twice with fresh egg water, deyolked, flash frozen in liquid nitrogen and stored at -...
ORGANISM(S): Danio rerio (Zebrafish) (Brachydanio rerio) 
2024-06-16 | PXD043679 | Pride
Acbd6 Whole proteome Danio rerio 
ProteomicsACBD6-related progressive neurodevelopmental disorder – whole proteome of developing Xenopus tropicalis
In quadruplicate for each condition, 10 acbd6 WT, 68 acbd6 crispant and 71 crispant X. tropicalis embryos were incubated with 20 μM YnMyr for 17 h. After labelling, embryos were washed twice with fresh 0.05 x MMR, flash frozen in liquid nitrogen and stored at -80°C until further analysis.
ORGANISM(S): Xenopus tropicalis 
2024-06-16 | PXD043680 | Pride
Acbd6 Xenopus tropicalis Whole proteome 
ProteomicsACBD6-related progressive neurodevelopmental disorder – chemical proteomics of YnMyr-labelled proteins in developing Danio rerio (zebrafish)
In triplicate for each condition, 12 WT and acbd6 F0 crispant Danio rerio (zebrafish) embryos were incubated with 20 μM YnMyr for 24 h, either between 48-72 hpf or 96-120 hpf. After labelling, zebrafish were washed twice with fresh egg water, deyolked, flash frozen in liquid nitrogen and stored at -...
ORGANISM(S): Danio rerio (Zebrafish) (Brachydanio rerio) 
2024-06-16 | PXD043676 | Pride
Acbd6 Danio rerio N-myristoylation Ynmyr 
ProteomicsACBD6-related progressive neurodevelopmental disorder – chemical proteomics of YnMyr-labelled proteins in developing Xenopus tropicalis
In quadruplicate for each condition, 10 acbd6 WT, 68 acbd6 crispant and 71 crispant X. tropicalis embryos were incubated with 20 μM YnMyr for 17 h. After labelling, embryos were washed twice with fresh 0.05 x MMR, flash frozen in liquid nitrogen and stored at -80°C until further analysis.
ORGANISM(S): Xenopus tropicalis 
2024-06-16 | PXD043677 | Pride
Acbd6 Xenopus tropicalis N-myristoylation Ynmyr 
ProteomicsEffects of age and sex on protein abundance in the mouse heart
We compared protein abundance in the left and right ventricles of male and female C57BL6/J mice at 4 months and 20 months old. Frozen tissues were weighed, thawed, and cut into small pieces (2-3 mm3) on weigh boats on ice. Tissue pieces were transferred to 2.0 mL eppendorf tubes. Cold RIPA (Thermo #...
ORGANISM(S): Mus Musculus (mouse) 
2022-11-08 | PXD033719 | JPOST Repository
UnknownA nebulised antitumour necrosis factor receptor-1 domain antibody in patients at risk of postoperative lung injury: A randomised, placebo-controlled pilot study.
Not available
S-EPMC7575021 | biostudies-literature
ProteomicsAmino acid and heavy water labelling to obtain protein turnover rates in intact adult animals
Both labelling studies were conducted with C57/BL6J adult male mice, derived from the same supplier and maintained under identical conditions. Eleven adult male C57BL/6JOlaHsd mice (Harlan UK Ltd., Shardlow, UK) of over 12 months of age at the start of the experiment were housed individually in 48 x...
ORGANISM(S): Mus Musculus (mouse) 
2021-12-13 | PXD029639 | JPOST Repository
UnknownAn Evaluation of Messages to Promote Parental Intent to Vaccinate Children Aged <12 Years Against COVID-19.
Not available
S-EPMC10851906 | biostudies-literature
ProteomicsPlasma Biomarkers for VX exposure in guinea pigs
Biomarker discovery using plasma obtained from exposed guinea pigs (N=6) exposed to VX.
ORGANISM(S): Cavia porcellus (Guinea pig) 
2020-08-03 | PXD017254 | Pride
Vx Cwa Guinea pig Biomarker 
UnknownDiscovery of treatment for nerve agents targeting a new metabolic pathway.
Not available
S-EPMC7415758 | biostudies-literature
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