This study aims to systematically investigate the molecular mechanisms underlying autism spectrum disorder (ASD) by analyzing the proteome and phosphoproteome of a Sh3rf3-deficient mouse model. SH3RF3 (SH3 domain-containing RING finger protein 3) is implicated in synaptic regulation pathways, yet it...
Sh3rf3 is an ASD-associated gene,Sh3rf3 deficiency reduces excitatory synaptic strength in prefrontal cortex and decreases the readily releasable vesicle pool and attenuated vesicle replenishment.To delve into the underlying mechanisms for impaired excitatory transmission, we performed immunoprecipi...
Analysis of MCA205 tumors developed in C57bl/6 mice on day 2, 7 and 10 after intratumoral treatment with doxorubicin (DX) or PBS solvent. DX triggers the immunogenicity of dying tumor cell and activates anti-tumor immunity. Results provided insight into molecular basis of DX induced immune response....
Bone marrow cells were isolated, primed with M-CSF (M-BMDM) or GM-CSF (GM-BMDM) and cultured for 7 days. The proteomic difference between GM-BMDM and M-BMDM were analyzed to describe the phenotye and function of two types of macrophages.
Cell membrane protein-protein interactions (PPIs) play pivotal roles in maintaining cellular homeostasis and physiological balance. Deciphering these interactions is crucial for understanding disease mechanisms and identifying therapeutic targets. While proximity labeling technologies have revolutio...
The temporal response of primary human alveolar adenocarcinoma epithelial cells (A549) infected with H5N1 influenza virus and H9N2 influenza A viruswere evaluated using the proteomics approaches (2D-DIGE combined with MALDI-TOF-MS/MS) at 24 hours post of the infection .
This project includes two parts of mass spectrometry-based omics analysis. First, peptidomics analysis was performed to identify endogenous free peptides from Ophiocordyceps sinensis. Second, quantitative proteomic analysis was applied to investigate the global protein expression changes in RAW 264....
The 4D label free proteomics of membrane vedsicles (MVs) of Staphylococcus aureus RN4220 and the mutants RN4220-ΔHld, which the 20th base “C” in the hld gene was changed to “G” to achieve a stop codon TGA and result in a truncated translation of Hld, and RN4220-ΔagrA, which agrA gene was deleted.
Immunoprecipitation coupled with mass spectrometry (IP/MS) was performed in HaCaT cells using an anti-B7-H3 antibody to identify B7-H3-interacting proteins. Raw mass spectrometry data are provided.
