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We developed a column-based CD9 antibody-immobilized HPLC immunoaffinity (CD9-HPLC-IAC) technology for EV isolation from a microliter-scale of serum for downstream proteomic analysis. The CD9-HPLC-IAC method achieved EV isolation from 40 μL of serum in 30 min with a yield of 8.0×109 EVs. Proteomic a...
ORGANISM(S): Homo sapiens (Human) 
2022-10-14 | PXD027025 | Pride
A comprehensive screening of site-specific N-glycopeptides in serum haptoglobin (Hp), a reporter molecule for aberrant glycosylation in HCC, has been performed to characterize glycopeptide markers for NASH-related HCCs.
ORGANISM(S): Homo sapiens (Human) 
2020-05-22 | PXD018502 | Pride
A workflow for differential analysis of the microheterogeneity of site-specific intact N-glycopeptides of serum haptoglobin between early hepatocellular carcinoma (HCC) and liver cirrhosis has been developed.
ORGANISM(S): Homo sapiens (Human) 
2018-10-25 | PXD011239 | Pride
To identify potential host factors promoting HIV-1 control and non-progressive infection, we employed mass spectrometry-based proteomic profiling to analyze three LTNPs and two PRs,in order to identify proteins expressed at higher levels in LTNPs.
ORGANISM(S): Homo Sapiens 
2024-05-30 | PXD050294 |
The whole blood samples were obtained from 3 LTNPs and 2 RPs HIV-1 infected patients, and the CD4+ T cells were isolated using anti-CD4-specific antibody-coated microbeads (130-045-101, Miltenyi Biotec, Germany) according to the manufacturer's instructions. Then the proteins extracted from the CD4+ ...
ORGANISM(S): Homo Sapiens 
2023-10-12 | PXD046078 |
We developed a multiplex pseudo-isobaric dimethyl labeling (m-pIDL) method for proteome quantification to extend the capacity of the fragment ion-based method to 6-plex by one-step dimethyl labeling with several millidalton and dalton mass differences between precursor ions and enlarging the isolati...
ORGANISM(S): Homo Sapiens Escherichia Coli 
2019-01-22 | PXD012457 |
Samples were analyzed using a nanoACQUITY UPLC system (Waters) coupled to a Q-Exactive HF-X high resolution accurate mass tandem mass spectrometer (Thermo) via a nanoelectrospray ionization source. Briefly the sample was first trapped on a Symmetry C18 180 micron x 20 mm trapping column (5 microlite...
ORGANISM(S): Nicotiana Tabacum (ncbitaxon:4097) 
2019-11-07 | MSV000084557 | MassIVE
Hair shaft is one of the most common biological evidences found at crime scene. Due to the low amount and high degradation of nuclear DNA in hair shaft, it is difficult to achieve individual identification through routine method. The proteins in the hair shaft are stable and contain genetic polymorp...
ORGANISM(S): Homo Sapiens 
2022-06-22 | PXD034814 |
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