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ProteomicsThe UBA-domain of Ubc1/Ube2K Facilitates the Assembly of K48-/K63-Branched Ubiquitin Chains
Assembly of polymeric ubiquitin (Ub) chains is an essential posttranslational protein modification that regulates widespread intracellular processes in eukaryotic cells. Factors and mechanisms that regulate the formation of Ub chains are key to the understanding of many cellular processes. The E2 en...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2021-09-09 | PXD018651 | Pride
Ubc1/ube2k ubiquitin k48-/k63-branched ubiquitin prm 
ProteomicsUbiREAD deciphers proteasomal degradation code of homotypic and branched K48 and K63 ubiquitin chains
Ubiquitin chains encode signals defining the fates of their modified proteins. Ubiquitin chain-mediated proteasomal degradation is a widespread eukaryotic regulatory mechanism. Yet, heterogeneity of intracellular ubiquitination has precluded systematically comparing degradation capacities of differe...
ORGANISM(S): Homo sapiens (Human) 
2025-06-23 | PXD060731 | Pride
Ubiquitin K48 Deubiquitination K63 Protein degradation Proteasome Proteostasis Electroporation Ubiquitin code Branched ubiquitin chains 
ProteomicsThe UBA domain of Ubc1 (Ube2K) Facilitates the Assembly of K48/K63-Branched Ubiquitin Chains
The assembly of a specific polymeric ubiquitin (Ub) chain on a target protein is a key event in the regulation of numerous cellular processes. Yet, the mechanisms that govern the selective synthesis of particular polyubiquitin signals remain enigmatic. The ubiquitin-conjugating (E2) enzymes Ubc1 in ...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2021-03-02 | PXD022621 | Pride
Ubiquitin Ubc1 Ubc4 Yeast Branched 
UnknownUbiREAD deciphers proteasomal degradation code of homotypic and branched K48 and K63 ubiquitin chains.
Not available
S-EPMC7617769 | biostudies-literature
UnknownATXN3 regulates lysosome regeneration after damage by targeting K48-K63-branched ubiquitin chains.
Not available
S-EPMC12436607 | biostudies-literature
UnknownVCP/p97-associated proteins are binders and debranching enzymes of K48-K63-branched ubiquitin chains.
Not available
S-EPMC11638074 | biostudies-literature
UnknownThe UBA domain of conjugating enzyme Ubc1/Ube2K facilitates assembly of K48/K63-branched ubiquitin chains.
Not available
S-EPMC7957398 | biostudies-literature
ProteomicsK48-K63-Ubiquitin chain pulldown from U2OS cell lysates
SpyTagged branched and unbranched K48- and K63-linked Tetra-Ubiquitin chains were immobilized on SpyCatcher agarose (48Ub4; 63Ub4; [Ub]2-48,63Ub-48Ub; [Ub]2-48,63Ub-63Ub). Pulldown were performed from protease-inhibitor treated U2OS cell lines to identify specific binders of K48-K63-branched Ub4.
ORGANISM(S): Homo sapiens (Human) 
2024-05-07 | PXD046025 | Pride
Human Lc-msms Dia Ubiquitin Branched ubiquitin 
ProteomicsComprehensive synthesis of K48/K63 ubiquitin pentamers by graph-empowered automation
Linear, branched, and heterotypic ubiquitin (Ub) chains regulate essential cellular processes, yet their structural and functional analysis remains limited by the difficulty of preparing defined, homogeneous samples by chemical synthesis or biochemical assembly. We established a fully automated plat...
ORGANISM(S): Homo sapiens (Human) 
2026-10-21 | PXD079080 | Pride
Ubiquitin 
UnknownATXN3 regulates lysosome regeneration after damage by targeting K48-K63-branched ubiquitin chains
Not available
S-SCDT-10_1038-S44318-025-00517-X | biostudies-other
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