Spatial and proteomic profiling reveals centrosome-independent features of centriolar satellites.

ABSTRACT: Centriolar satellites are small electron-dense granules that cluster in the vicinity of centrosomes. Satellites have been implicated in multiple critical cellular functions including centriole duplication, centrosome maturation, and ciliogenesis, but their precise composition and assembly properties have remained poorly explored. Here, we perform in vivo proximity-dependent biotin identification (BioID) on 22 human satellite proteins, to identify 2,113 high-confidence interactions among 660 unique polypeptides. Mining this network, we validate six additional satellite components. Analysis of the satellite interactome, combined with subdiffraction imaging, reveals the existence of multiple unique microscopically resolvable satellite populations that display distinct protein interaction prof

SUBMITTER: Gheiratmand L 

PROVIDER: S-EPMC6627244 | biostudies-literature | 2019 Jul

REPOSITORIES: biostudies-literature