Transcriptome profiling of B16F10 cells and differentiated B16F10 cells induced by forced expresssion of Myod1, Pparg, or by knockdown of Setdb1 using RNA-sequencing.
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ABSTRACT: The core proeprty of cancer cells is neural stemness, which determines tumorigenicity and pluripotency. Therefore, cancer cells can supposedly be induced to differentiate along different lineages. B16F10 is a mouse melanoma cell line. We found that B16F10 cells differentiated into muscle cells or adipocytes upon induction by muscle or adipocyte differentiation factor Myod1 or Pparg, respectively. Moreover, Setdb1 is an epigenetic modification factor responsible for catalyzing the histone modification H3K9me3. Transcription of Setdb1 gene is enriched in embryonic neural cells during vertebrate embryogenesis. Setdb1 is upregulated in cancer cells and promotes cancers. We found that knockdown of Setdb1 in B16F10 cells led to a neuronal-like differentiation phenotype.
ORGANISM(S): Mus musculus
PROVIDER: GSE295152 | GEO | 2026/06/01
REPOSITORIES: GEO
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