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Ablation of ZC3H11A causes early embryonic lethality and dysregulation of metabolic processes

ABSTRACT: In human somatic cells, ZC3H11A has been recently characterized as an RNA-export protein that functions through its interaction with TREX complex proteins. In order to identify its interacting partners in embryonic cells, and to investigate if ZC3H11A maintains its association with the TREX complex in mouse embryonic stem cells (mESCs), we have performed co-immunoprecipitations (co-IPs) using anti-ZC3H11A, anti-THOC2 and anti-IgG antibodies followed by mass spectrometry (MS) analyses. Statistical analyses of detected MS intensities from the biological replicates (n=4) have revealed a number of proteins with statistically significant interaction with ZC3H11A and THOC2. Results in xlsx format contain the log-fold change in protein intensities in the ZC3H11A co-IP relative to the IgG co-IP and is presented along with the adjusted P-values.

INSTRUMENT(S): Orbitrap Fusion ETD

ORGANISM(S): Mus Musculus (ncbitaxon:10090)

SUBMITTER: Shady Younis Marten Larsson

PROVIDER: MSV000091811 | MassIVE | Thu Apr 27 08:29:00 BST 2023

SECONDARY ACCESSION(S): PXD041852

REPOSITORIES: MassIVE

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