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ProteomicsDetectability of biotin tags in LC-MS/MS
Raw files, corrected search engine files, and sequence database files for "Detectability of Biotin Tags by LC-MS/MS". Custom PTMs were added for the modifications that result from EZ-Link Sulfo-NHS-SS-Biotin that were not available in the database.
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2021-03-10 | MSV000087032 | MassIVE
ProteomicsStructural mapping of the surfaces of fibrillar 1N3R and 1N4R human tau
The structural characterization of pathogenic tau filaments that accumulate in tauopathies, including Alzheimer disease, is key for understanding their pathogenic function and identify the structural determinants of distinct tau filaments involved in distinct tauopathies. Fibrillar tau surfaces play...
ORGANISM(S): Homo sapiens (Human) 
2022-02-17 | PXD027403 | Pride
Structural proteomics  proteolysis  chemical surface modification  nhs-biotin  nanolc-msms Tau fibrils 
ProteomicsUnveiling Cellular Communications through Rapid Pan-Membrane-Protein Labeling
The dynamic protein distribution within and across the plasma membrane is pivotal in regulating cell communications. Yet, methods facilitating rapid labeling, applicable across diverse cell types, and amenable to multiplexed live imaging are scarce. We discovered that live mammalian cell surface pro...
ORGANISM(S): Mus musculus (Mouse) 
2025-02-28 | PXD061317 | Pride
Mouse Nhs-biotin Plasma membrane 
ProteomicsUltrafast and Chemoselective Biotinylation of Living Cell Surface for Time-Resolved Surfaceome Analysis - final
Cell surface proteins participate in many important biological processes such as cell-to-cell interaction, signal transduction, cell adhesion, and protein transportation. In-depth study of the cell surface protein group is of great significance. Nevertheless, detection and analysis of the surfaceome...
ORGANISM(S): Homo Sapiens 
PXD053733 |
ProteomicsHuman serine/threonine protein kinase ATR LC-MS/MS
ATR is a PI3K-like kinase protein, regulating checkpoint responses to DNA damage and replication stress. Apart from its checkpoint function in the nucleus, ATR actively engage in antiapoptotic role at mitochondria following DNA damage. The different functions of ATR in the nucleus and cytoplasm are ...
ORGANISM(S): Homo sapiens (Human) 
2022-08-12 | PXD031385 | Pride
Biotin modification Cis/trans prolyl isomerization Mass protein footprinting Bh3-like domain Atr 
ProteomicsA surface biotinylation strategy for reproducible plasma membrane protein purification and tracking of genetic and drug-induced alterations
In the past years several protocols for the proteomic profiling of plasma membrane proteins have been described. Nevertheless, comparative analyses have mainly focused on different variations of one approach [1-3]. To pave the way to high-performance differential plasma membrane proteomics, we compa...
ORGANISM(S): Homo sapiens (Human) 
2022-02-22 | PXD002141 | Pride
Kbm7 Mcp Technical paper Cell surface proteomics 
ProteomicsLuminal cerebrovascular proteomic changes during aging
The blood-brain barrier (BBB) is highly specialized to protect the brain from harmful circulating factors in the blood and maintain brain homeostasis. However, during aging and neurodegenerative disease, the BBB undergoes numerous molecular changes that can promote inflammation and disease pathogene...
ORGANISM(S): Mus musculus (Mouse) 
2024-12-17 | PXD043964 | Pride
Brain Mouse Vasculature Bbb Aging 
ProteomicsCell Surface Biotinylation via Furan Crosslinking to Profile the Surfaceome
The cellular surfaceome is crucial for cellular homeostasis and communication, with surface proteins acting as key receptors, transporters, and enzymes. However, comprehensive analysis of the surfaceome is challenging due to the hydrophobic nature and low abundance of membrane proteins, resulting un...
ORGANISM(S): Homo sapiens (Human) 
2025-10-21 | PXD059552 | Pride
Proteomics Affinity purification Furan-biotin Plasma membrane proteins Lc-ms/ms Surfaceome 
ProteomicsTarget identification for 11q13 amplicon
Proteomic strategy to define therapeutically relevant targets in cell lines that contain the 11q13 amplicon compared to those that do not and to ascertain which genes are amplified at the protein level and, concomitantly, are key drivers for tumor growth and/or maintenance. Furthermore, so called p...
ORGANISM(S): Homo sapiens (Human) 
2015-08-04 | PXD002486 | Pride
Human Lc-msms Head and neck Cell surface protein enrichment Breast 11q13 Liver Oscc Tmt6 quantitation 
ProteomicsGlobal surface proteomes of hPSC-derived brain microvascular endothelial-like cells (hPSC-BMELCs), human cerebral microvascular endothelial cell line D3 (HCMEC/D3), and human umbilical vein endothelial cells (HUVECs)
Abundance proteomics analysis was performed to characterize the surface proteome of three cell lines: hPSC-BMELCs, a stem cell derived model for human blood-brain barrier endothelial cells; HCMEC/D3, an immortalized cell line of brain microvascular endothelial cells; and HUVECs, primary human endoth...
ORGANISM(S): Homo sapiens (Human) 
2025-11-09 | PXD059899 | Pride
Abundance proteomics Receptors Endothelial cells Surface proteome 
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