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MetabolomicsMapping the biosynthetic pathway of a hybrid polyketide-nonribosomal peptide in a metazoan
Polyketide synthase (PKS) and nonribosomal peptide synthetase (NRPS) hybrid systems typically use complex protein-protein interactions to facilitate direct transfer of intermediates between these multimodular megaenzymes. In the canal-associated neurons (CANs) of Caenorhabditis elegans, PKS-1 and NR...
2021-06-22 | MTBLS2313 | MetaboLights
ProteomicsRapid Highly-Efficient Digestion and Peptide Mapping of Adeno-Associated Viruses
[15:18] Toole, Estee Adeno-Associated Viruses (AAVs) comprise an area of rapidly growing interest due to their ability to act as a gene delivery vehicle in novel gene therapy strategies and vaccine development. Peptide mapping is a common technique in the biopharmaceutical industry to confirm ...
ORGANISM(S): Adeno-associated virus - 2 
2022-02-17 | PXD025971 | Pride
Viral therapies Pepsin Trypsin [15:18] toole Aav Peptide mapping Proteolysis Nanolc Estee gene therapy 
ProteomicsFast & High Throughput LC-MS Characterization, and Peptide Mapping of Engineered AAV Capsids Using LC-MS/MS
Adeno-associated virus (AAV) has emerged as a leading platform for gene therapy. With a skyrocketing rate of AAV research and the prevalence of many new engineered capsids being investigated in preclinical and clinical trials, capsid characterization plays an important role in serotype confirmation ...
ORGANISM(S): Adeno-associated virus 9 
2023-03-11 | PXD035837 | Pride
Peptide map. Aav capsids Engineered aav capsids Lcms Msms 
ProteomicsChemical Targeting of the Redox Switch in the Transcription Factor ΔFOSB
Purified protein fragments (~8 kDa bZIP domains, ΔFOSB and JunD) were reacted with individual compounds selected from a previous library screen. Reacted proteins were analyzed by LC-MS/MS peptide mapping and LC-MS intact mass analysis.
ORGANISM(S): Homo sapiens (Human) 
2022-10-14 | PXD035839 | Pride
Compound screening peptide mapping intact mass 
ProteomicsMyeloid cell-mediated killing of BCP-ALL by IgA2 variants of CD38 or CD20 antibodies is enhanced by CD47/SIRPα interference
Characterization of therapeutic monoclonal antibodies used in the context of B-cell precursor acute lymphoblastic leukemia (BCP-ALL). IgA2 antibodies targeting CD38 were analyzed using LC-MS/MS. Mass analysis, peptide mapping and glycoprofiling were performed to confirm sequence integrity and post-t...
ORGANISM(S): Homo sapiens (Human) 
2026-06-09 | PXD066660 | Pride
Peptide mapping Lc-ms Glycoprofiling Daratumomab-iga 
TranscriptomicsRNA sequencing of HEK293T cells expressing peptide 9J10, a 14-3-3 interacting peptide
We investigated the transcriptome profile of HEK293T cells transfected with a plasmid encoding 9J10, a peptide isolated in a phenotypic screen from a library of peptides derived from bacterial and archaeal genomes. The peptide was identified in a screen for FOXO3a reactivation and has been shown to...
ORGANISM(S): Homo sapiens 
2021-05-28 | E-MTAB-10161 | biostudies-arrayexpress
ProteomicsNIST mAb High Throughput Peptide Mapping With Cyclic IM-MS
Peptide mapping datasets for NIST mAb using 15-s and 1-min LC gradient separations coupled to Cyclic IM-MS.
ORGANISM(S): Antibody Standard 
2025-06-15 | MSV000098206 | MassIVE
ProteomicsLC-MS peptide mapping of monoclonal antibodies using the mirror proteases trypsin and Tryp-N
Accurate characterization of the amino acid sequence and post-translational modifications (PTMs) of monoclonal antibodies (mAbs) is essential for evaluating product quality. Peptide mapping through bottom-up LC/MS analysis is a key methodology for this purpose. While trypsin is commonly the first ch...
ORGANISM(S): Homo Sapiens (human) 
2026-04-15 | PXD072803 | JPOST Repository
ProteomicsHarnessing the power of electrophoresis and chromatography: Offline coupling of reverse phase liquid chromatography-capillary zone electrophoresis-tandem mass spectrometry for peptide mapping for monoclonal antibodies
RPLC-MS/MS is the present workhorse for bottom-up proteomics-based characterization. However it suffers from limited peak capacity and challenges with respect to detection of small and hydrophilic peptides. CZE-MS/MS offers an orthogonal alternative to RPLC-MS/MS but has limited sensitivity due to l...
ORGANISM(S): Mus musculus (Mouse) 
2020-03-05 | PXD017316 | Pride
Biotherapeutics Peptide mapping Rplcmsms Czemsms 
ProteomicsMapping the HLA ligandome landscape of acute myeloid leukemia: a targeted approach toward peptide-based immunotherapy
Identification of physiologically relevant peptide vaccine targets calls for the direct analysis of the entirety of naturally presented human leukocyte antigen (HLA) ligands, termed the HLA ligandome. In this study, we implemented this direct approach using immunoprecipitation and mass spectrometry ...
ORGANISM(S): Homo sapiens (Human) 
2019-10-14 | PXD015748 | Pride
Acute myeloid leukemia Lc-msms 
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