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ProteomicsSelf-priming of Plk1 binding to BubR1 ensures accurate mitotic progression
Plk1 is a key mitotic kinase that localizes to distinct subcellular structures to promote accurate mitotic progression. Plk1 recruitment depends on direct interaction between polo-box domain (PBD) on Plk1 and PBD binding motif (PBD BM) on the interactors. However, recent study showed that PBD BM alo...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2024-10-31 | MSV000096264 | MassIVE
TranscriptomicsPDK1 Signaling Towards PLK1-Myc Activation Confers Oncogenic Transformation and Tumor Initiating Cell Activation and Resistance to mTOR-targeted Therapy.
Although 3-Phosphoinositide-dependent protein kinase-1 (PDK1) has been predominately linked to PI3K-AKT pathway, it may also evoke additional signaling outputs to promote tumorigenesis. Here we report that PDK1 directly induces phosphorylation of Polo-like kinase 1 (PLK1), which in turn induces Myc ...
ORGANISM(S): Homo sapiens 
2013-07-31 | E-GEOD-30669 | biostudies-arrayexpress
ProteomicsIdentifying the mitotic interactome of the human PLK1 protein kinase
The project mapped the interactors of PLK1 in cells were the endogenous PLK1 locus was tagged with 3xHA tags. Cells were arrested in mitosis with nocodazole and treated with a PLK1 inhibitor (BI-2536) to trap PLK1 onto its substrates, facilitating detection by mass spectrometry.
ORGANISM(S): Homo sapiens (Human) 
2026-07-03 | PXD080479 | Pride
Plk1 mitotic interactome 
ProteomicsThe Eyes Absent family members EYA4 and EYA1 promote PLK1 activation and successful mitosis through tyrosine dephosphorylation: PLK1 PRM
The aim of these experiments was to quantify PLK1 tyrosine phosphosites with respect to EYA4 and EYA1. PRM was performed on immunopurified PLK1. This was part of a larger project to characterize the effects of EYA4 mediated dephosphorylation on PLK1 functions.
ORGANISM(S): Homo sapiens (Human) 
2024-02-26 | PXD037064 | Pride
Plk1 prm 
ProteomicsIdentification of Plk1 interacting proteins by XChIP-MS
Polo-like kinase 1 (Plk1) is an important protein kinase for checkpoint recovery and adaptation in response to DNA damage and replication stress. However, although Plk1 is present in S phase, little is known about its localization and function during unperturbed DNA replication. Using Xenopus laevis...
ORGANISM(S): Xenopus laevis (African clawed frog) 
2021-08-25 | PXD022440 | Pride
Lc-msms Xenopus laevis 
ProteomicsProteomic Analysis of a PLK1-Dependent, Cell Cycle Degradation Program
Targeted protein degradation by the ubiquitin-proteasome system is an essential mechanism regulating cellular division. The kinase PLK1 coordinates protein degradation at the G2/M phase of the cell cycle by promoting the binding of substrates to the E3 ubiquitin ligase SCFβTrCP. However, the magnitu...
ORGANISM(S): Homo sapiens (Human) 
2025-05-06 | PXD046039 | Pride
Degradation Plk1 Cell cycle Ubiquitin 
ProteomicsMass spectrometry analysis of in vitro PLK1 phosphorylation
Polo-like kinase 1 (PLK1) protects against genome instability by ensuring timely and accurate mitotic cell division, and its activity is tightly regulated throughout the cell cycle. Although the pathways that initially activate PLK1 in G2 are well-characterized, the factors that directly regulate PL...
ORGANISM(S): Homo sapiens (Human) 
2024-11-01 | PXD053933 | Pride
Kinase Prm Phosphorylation 
ProteomicsPLK1 inhibition induces synthetic lethality in Fanconi anemia pathway-deficient acute myeloid leukemia
Overall survival of acute myeloid leukemia (AML) remains limited. Inhibitors of the master mitotic kinase PLK1 have emerged as promising therapeutics, demonstrating efficacy in an undefined subset of AML patients. However, the clinical success of PLK1 inhibitors remains hindered by a lack of predict...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2024-12-09 | MSV000096625 | MassIVE
TranscriptomicsPLK1 signaling coopereates with estrogen receptor-dependent gene transcription.
Analysis of MCF7 breast cancer cells treated with estadiol for 6 h in presence or absence of the specific PLK1 inhibitor BI2536. Together with CHIP and global phosphoproteome data, the results demonstrate a key role of PLK1 in the estrogen receptor-mediated gene response. Hormone-deprived MCF7 cells...
ORGANISM(S): Homo sapiens 
2014-01-01 | E-GEOD-46856 | biostudies-arrayexpress
ProteomicsPRC1 phosphorylation by CDK1 and PLK1
This work study the effects of PRC1 phosphorylation by CDK1 and PLK1.
ORGANISM(S): Homo sapiens (Human) 
2024-11-01 | PXD055933 | Pride
Prc1 Cdk1 Plk1 
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