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ProteomicsNano LC MS/MS of cell wall extracts from Lactiplantibacillus plantarum strains
Strains of Lactiplantibacillus plantarum treated with lithium chloride to extract non-covalently bound cell wall proteins that were identified by mass spectrometry proteomic analysis.
ORGANISM(S): Lactobacillus plantarum 
2026-02-09 | PXD063717 | Pride
Lc-msms Proteomics Cell wall Plantarum Lactobacillus 
MetabolomicsInositol triphosphate-triggered calcium release blocks lipid exchange at endoplasmic reticulum-Golgi contact sites
Vesicular traffic and membrane contact sites between organelles enable the exchange of proteins, lipids, and metabolites. Recruitment of membrane tethers to contact sites between the endoplasmic reticulum (ER) and the plasma membrane is often triggered by calcium. In contrast, we reveal here a funct...
2021-05-06 | MTBLS2444 | MetaboLights
ProteomicsProteomics-based insights into mitogen-activated protein kinase inhibitor resistance of cerebral melanoma metastases
Background MAP kinase inhibitor (MAPKi) therapy for BRAF mutated melanoma is characterized by high response rates but also development of drug resistance within a median progression-free survival (PFS) of 9 to 12 months. Understanding mechanisms of resistance and identifying effective therapeutic al...
ORGANISM(S): Homo sapiens (Human) 
2018-03-16 | PXD007592 | Pride
Melanoma Cerebral melanoma metastases Drug resistance Map kinase inhibitor Proteomics Braf mutation 
ProteomicsSecretome profiling of Artemisia absinthium extract loaded polymeric nanoparticles-treated MCF-7 and MDA MB-231 cell lines.
Temperature and pH-sensitive, self-assembled polymeric nanoparticles, made of N-isopropyl acrylamide (NIPAAM), N-vinyl pyrrolidone (VP), and acrylic acid (AA) are biocompatible and have been used in the past with several drugs for site-specific delivery of different formulations. In the present stud...
ORGANISM(S): Homo sapiens (Human) 
2023-08-29 | PXD042365 | Pride
Artemisia absinthium Mda mb-231 Polymeric nanoparticles Mcf-7 Lc-ms/ms 
ProteomicsIdentification of Arabidopsis mitochondrial ribosome proteome
In order to identify the comprehensive composition of Arabidopsis mitochondrial ribosome proteins, a strategy based on complementary approaches was used. Classical biochemical purification of ribosomes was combined with the immunoprecipitation of mitoribosomes using a specific Arabidopsis mitoriboso...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2018-12-14 | PXD010324 | Pride
Arabidopsis Ribosome Mitochondria Nanolc-esi-ms/ms 
ProteomicsData on proteome of Mycoplasma hominis cultivated with arginine or thymidine as a carbon source
M. hominis cells were grown in liquid medium supplied with arginine or thymidine as a carbon source. LC-MS analysis was performed on Ultimate 3000 Nano LC System (Thermo Fisher Scientific) coupled with Q Exactive HF benchtop Orbitrap mass spectrometer (Thermo Fisher Scientific) via a nanoelectrospra...
ORGANISM(S): Mycoplasma hominis ATCC 23114 
2020-05-06 | PXD018714 | Pride
Growth conditions Hominis Proteome Mycoplasma 
ProteomicsFunctional cross-talk between allosteric effects of activating and inhibiting ligands underlies PKM2 regulation.
Allosteric regulation is central to the role of the glycolytic enzyme pyruvate kinase M2 (PKM2) in cancer metabolism. Multiple activating and inhibitory allosteric ligands regulate PKM2 activity by controlling the equilibrium between high activity tetramers and low activity dimers and monomers. Howe...
ORGANISM(S): Homo sapiens (Human) 
2019-11-12 | PXD010334 | Pride
Srm Prm Pkm2 Quantitation Qexactive 
ProteomicsQuantification of cytokines secreted by primary human cells using MRM: evaluation of analytical parameters – shotgun secretome data of untreated HUVECs
The determination of secreted proteins may provide highly valuable information about cell functions. While the typical methods for the determination of biologically relevant but low-abundant molecular species still relies on the use of specific antibodies, mass spectrometry-based methods are now gai...
ORGANISM(S): Homo sapiens (Human) 
2015-06-29 | PXD002212 | Pride
Proteome profiling Inflammation Primary human cells Cytokines Analytical assay Targeted proteomics 
ProteomicsQuantification of cytokines secreted by primary human cells using MRM: evaluation of analytical parameters – shotgun secretome data of inflammatory stimulated HUVECs
The determination of secreted proteins may provide highly valuable information about cell functions. While the typical methods for the determination of biologically relevant but low-abundant molecular species still relies on the use of specific antibodies, mass spectrometry-based methods are now gai...
ORGANISM(S): Homo sapiens (Human) 
2015-06-29 | PXD002211 | Pride
Proteome profiling Inflammation Primary human cells Cytokines Analytical assay Targeted proteomics 
ProteomicsThymic epithelial cells from WT and AB0/0 mouse model
Murine TEC for proteomic analyses were obtained from 3 pools of WT or AB0/0 mice, 3 mice per pool (5-6 week-old). TEC were isolated, enriched and processed through CD45+ cell-depletion as described above. CD45- cell-samples were pelleted and stored at -80 C until use. Murine CD45- TEC samples were ...
ORGANISM(S): Mus Musculus (ncbitaxon:10090) 
2021-02-15 | MSV000086866 | MassIVE
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