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MetabolomicsIlluminating the dark space of neutral glycosphingolipidome by selective enrichment and profiling at multi-structural levels

Glycosphingolipids (GSLs) are essential components of cell membranes, particularly enriched in the nervous system. Altered molecular distributions of GSLs are increasingly associated with human diseases, emphasizing the significance of lipidomic profiling. Traditional GSL analysis methods are ham...

2024-06-25 | MTBLS10400 | MetaboLights
ProteomicsAmorphous TiO2 Nanotubes as Platform for Highly Selective Phos-phopeptide Enrichment
This work reports highly selective phosphopeptide enrichment using amorphous TiO2 nanotubes (TiO2NTs) and the same material decorated with superparamagnetic Fe3O4 nanoparticles (TiO2NTs@Fe3O4NPs). TiO2NTs and TiO2NTs@Fe3O4NPs materials were applied for phosphopeptide enrichment both from a simple pe...
ORGANISM(S): Homo sapiens (Human) 
2019-06-26 | PXD012749 | Pride
Phosphopeptide enrichment Nanotubes 
ProteomicsSelective TiO2-based enrichment methods for analyzing phosphopeptide content of small-size complex samples
Comparison of several TiO2-based phosphopeptide enrichment methods
ORGANISM(S): Homo sapiens (Human) 
2020-12-17 | PXD019439 | Pride
Tio2 Hela Enrichment Phosphopeptide 
ProteomicsSsimultaneous enrichment of cysteine-containing peptides and phosphopeptides using a novel Cysteine-specific Phosphonate Adaptable Tag (CysPAT) in combination with TiO2 chromatography
Cysteine is a rare and conserved amino acid involved in most cellular functions. The thiol group of cysteine can be subjected to diverse oxidative modifications that regulate most physio-pathological states. Here, a Cysteine-specific Phosphonate Adaptable Tag (CysPAT) was synthesized to selectively ...
ORGANISM(S): Homo sapiens (Human) 
2016-06-13 | PXD002704 | Pride
Cyspat  reversible cys modifications  redox chemistry  protein phosphorylation  tio2 enrichment  cross-talk  proteomics 
ProteomicsZebrafish, LC-MS/MS - Profiling of Histidine Phosphoproteome in Danio rerio by TiO2 Enrichment
Histidine phosphorylation is a reversible post-translational modification that is known to regulate signal transduction in prokaryotes. In an effort to help elucidate the heretofore hidden vertebrate phosphoproteome, this report presents a global phosphorylation analysis of Danio rerio (zebrafish) l...
ORGANISM(S): Danio rerio (Zebrafish) (Brachydanio rerio) 
2019-03-21 | PXD012735 | Pride
Lc-ms/ms Embryo Zebrafish 
ProteomicsPhosphopeptide enrichment on synthetic libraries and HeLa cells digest
2 phosphopeptide enrichment methods (TiO2 and Ti-IMAC) were evaluated on synthetic libraries and HeLa cells digest. The resulting peptides were compared to the ones without enrichment with regards to the nature of the phosphorylation site (serine, threonine, tyrosine) and the characteristics of the ...
ORGANISM(S): Homo sapiens (Human) 
2014-08-11 | PXD000759 | Pride
Phosphopeptide enrichment Tio2 Ti-imac Hela Synthetic library 
ProteomicsRobust, reproducible, and economical phosphopeptide enrichment using calcium titanate.
We compare different preparations of TiO2 for phosphopeptide enrichment and demonstrate the efficacy of a method using CaTiO3
ORGANISM(S): Homo sapiens (Human) 
2019-01-02 | PXD011145 | Pride
Phosphoproteomics Enrichment Phosphopeptide 
ProteomicsAutomated Phosphopeptide Enrichment for Gram Positive Bacteria
Protein phosphorylation in prokaryotes has gained more attention in recent years as several studies linked it to regulatory and signalling functions indicating an importance similar to protein phosphorylation in eukaryotes. Studies on bacterial phosphorylation have so far been conducted using manual...
ORGANISM(S): Listeria monocytogenes EGD 
2021-08-26 | PXD025459 | Pride
Bravo assaymap  fe(iii)-imac  tio2  automation  phosphopeptide enrichment  phosphoproteomics  quantification  listeria monocytogenes  bacillus subtilis  streptococcus pyogenes 
ProteomicsReproducible Automated Phosphopeptide Enrichment using Magnetic TiO2 and Ti-IMAC
We describe the application of magnetic TiO2 and Ti-IMAC for uniform automated phosphopeptide enrichment. Combining hyper-porous magnetic microspheres with a magnetic particle-handling robot enables rapid (45 minutes), reproducible (r2 = 0.80) and high-fidelity (> 90% purity) phosphopeptide purifica...
ORGANISM(S): Mus musculus (Mouse) 
2014-09-22 | PXD000892 | Pride
Phosphoproteomics automation 
ProteomicsComprehensive evaluation of different TiO2-based phosphopeptide enrichment and fractionation methods for phosphoproteomics
We performed a comprehensive evaluation of four different TiO2-based phosphopeptide enrichment methods using different non-phosphopeptide excluders. Then, two phosphopeptide fractionation methods, including the ammonia-based and the TEA based high pH reversed-phase fractionation, are also evaluated.
ORGANISM(S): Homo Sapiens 
2022-06-02 | PXD034264 |
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