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ProteomicsAn in-depth Mass Spectrometry Characterization of the SDS-PAGE Protein Profile of Legumins and Vicilins from Chickpea Seed
Chickpea (Cicer arietinum L.) seed proteins show a lot of functional properties leading this leg-ume an interesting component for the development of protein-enriched foods. However, both in-depth proteomic investigation and structural characterization of chickpea proteins seed are still lacking. In ...
ORGANISM(S): Cicer arietinum (Chickpea) (Garbanzo) 
2024-06-23 | PXD049382 | Pride
Chickpea proteins  legumins  vicilins  sds-page  orbitrap® mass spectrometry 
ProteomicsLabel-free quantitative phosphoproteomics of ΔB-Raf:ER cells treated with U0126 or 4-HT
ΔB-Raf:ER cells were treated with 10 µM U0126 or 1 µM 4-HT for 30 min. The cells were lysed in 6 M guanidine-HCl containing 100 mM HEPES-NaOH (pH7.5), 10 mM TCEP, and 40 mM CAA. The lysates were dissolved by heating and sonication and then centrifuged. The supernatants were recovered, and proteins (...
ORGANISM(S): Mus Musculus (mouse) 
2022-03-03 | PXD028723 | JPOST Repository
ProteomicsIdentification of proteins in SDS-PAGE gel by LC-MSMS
Recombinant antibodies were purified from culture supernatants derived from the Dictyostelium discoideum model and loaded into an SDS-PAGE electrophoresis gel. The electrophoretic profile of the anti-alpha tubulin, anti-CISD1, and anti-CD8β antibodies reveals a band at 35 kDa, likely corresponding t...
ORGANISM(S): Dictyostelium discoideum (Slime mold) 
2025-04-11 | PXD053343 | Pride
Dictyostelium discoideum Recombinant antibodies 
UnknownHFIP extraction followed by 2D CTAB/SDS-PAGE separation: a new methodology for protein identification from tissue sections after MALDI mass spectrometry profiling for personalized medicine research.
Not available
S-EPMC4048577 | biostudies-literature
ProteomicsMytilus galloprovincialis, sperm, LC-MSMS
Sperm contains essential proteins for interaction with eggs, however, there are only several sperm proteins reported with important role in fertilization, and gamete proteomics are limited in marine invertebrate species. We present here a sperm proteomic profile of marine mussel Mytilus galloprovinc...
ORGANISM(S): Mytilus galloprovincialis (Mediterranean mussel) 
2015-07-17 | PXD001665 | Pride
Lc-msms Sperm Mytilus galloprovincialis 
ProteomicsDeep Proteomic Profiling of Vasopressin-Sensitive Collecting Duct Cells
The mouse mpkCCD cell line is a continuous cultured epithelial cell line with characteristics of renal collecting duct principal cells. This line is widely used to study epithelial transport and its regulation. To provide a data resource useful for experimental design and interpretation in studies ...
ORGANISM(S): Mus musculus (Mouse) 
2015-10-06 | PXD002434 | Pride
Sds-page Kidney cortical collection duct Mass spectrometry Aquaporin-2 Differential centrifugation Lc-ms/ms 
ProteomicsAspirin Acetylates Thioredoxin Reductase 1 (isoform 4)
Thioredoxin Reductase 1 (isoform 4) was incubated with aspirin for 1 hour at 37 C followed by SDS PAGE. Samples were picked from SDS PAGE gel, digested with trypsin, and sent for LC-MS/MS.
ORGANISM(S): Homo sapiens (Human) 
2022-02-17 | PXD027882 | Pride
Thioredoxin reductase Acetylation Aspirin 
ProteomicsComparison of protein and peptide fractionation approaches in protein identification and quantification from Saccharomyces cerevisiae
Proteomics is a high-throughput technology which has been developed with the aim of investigating the maximum number of proteins in cells in a given experiment. However, protein discovery and data generation vary in depth and coverage when different technical strategies are selected. In this study, ...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2020-11-30 | PXD018854 | Pride
High ph reversed phase fractionation Sds-page Gpf Yeast Fasp Proteome 
ProteomicsAnalysis of mixed-oxysterol treatment on the THP-1 macrophage
THP-1 monocytes were differentiated, via PMA treatment, to macrophages. THP-1 macrophages were then treated with a mixed-oxysterol treatment (7-ketocholesterol and 7beta-hydroxycholesterol) for 24 hours. The mixed-oxysterol treatment was mixed to a ratio that represents the cytotoxic oxysterol conte...
ORGANISM(S): Homo sapiens (Human) 
2017-03-15 | PXD004304 | Pride
Thp-1 Macrophage Proteomics Oxysterol 2-de Tandem-mass spectrometry 
ProteomicsETFDH comigrates with CIII in BN-PAGE
Proteomic analysis of 13 bands from BN-PAGE immunostaining of mouse skeletal muscle mitochondrial proteins: Coenzyme Q (Q) is a key lipid electron transporter, but several aspects of its biosynthesis and redox homeostasis remain undefined. Various flavoproteins reduce the Q; however, in eukaryotes, ...
ORGANISM(S): Mus musculus (Mouse) 
2023-10-24 | PXD045352 | Pride
Coenzyme q  oxphos-complex iii  skeletal muscle  mitochondria 
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