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ProteomicsAffinity capture of S. cerevisiae protein after glutaraldehyde stabilization
It remains extraordinarily challenging to elucidate endogenous protein-protein interactions and proximities within the cellular milieu. The dynamic nature and the large range of affinities of these interactions augment the difficulty of this undertaking. Among the most useful tools for extracting su...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2018-10-19 | PXD001262 | Pride
Budding yeast Glutaraldehyde Affinity capture 
ProteomicsProteomic profiling of centrosomes across multiple cell and tissue types by a new affinity capture method
Centrosomes are small cytoplasmic organelles that play fundamental roles in a range of cellular processes. Nearly all vertebrate cell types contain centrosomes, but whether centrosome composition and function differ between cell types, tissues and pathologies remains an outstanding question. Until n...
ORGANISM(S): Mus musculus (Mouse) Homo sapiens (Human) 
2023-10-18 | PXD040308 | Pride
Proteomics Capture peptide Capture-ms Centrosomes 
ProteomicsProteomic profiling of centrosomes across multiple cell and tissue types by a new affinity capture method
Centrosomes are small cytoplasmic organelles that play fundamental roles in a range of cellular processes. Nearly all vertebrate cell types contain centrosomes, but whether centrosome composition and function differ between cell types, tissues and pathologies remains an outstanding question. Until n...
ORGANISM(S): Homo sapiens (Human) 
2023-10-18 | PXD040309 | Pride
Proteomics Centrosomes Capture ms 
ProteomicsMulti-condition affinity capture/MS analysis of LAP-tagged human NCBP1, NCBP2, and NCBP3
A 5′, 7-methylguanosine cap is a quintessential feature of RNA polymerase II-transcribed RNAs, and thus a textbook aspect of co-transcriptional pre-mRNA processing. The cap is bound by the cap-binding complex (CBC), canonically consisting of nuclear cap-binding proteins 1 and 2 (NCBP1/2). Recently, ...
ORGANISM(S): Homo sapiens (Human) 
2020-11-09 | PXD016038 | Pride
Mrnp Cap binding complex Cbc Interactome screen 
Proteomics14-3-3 affinity capture to identify new targets of 14-3-3 signaling during dsRNA-stimulation in human keratinocytes.
We have combined two proteomic approaches, phosphoproteomics and 14-3-3 affinity capture, to examine the signaling pathways in which 14-3-3 proteins participate and to identify new targets of 14-3-3 signaling during dsRNA-stimulation.
ORGANISM(S): Homo sapiens (Human) 
2014-08-05 | PXD000677 | Pride
Human Itraq Hacat keratinocytes Lc-ms/ms 
ProteomicsCombining Affinity Enrichment, Cross-Linking with Photo-Amino Acids, and Mass Spectrometry for Probing Protein Kinase D2 Interactions
We present an approach that relies on the affinity enrichment of a target protein from a complex mixture, followed by a UV-induced activation of incorporated diazirine photo-reactive amino acids (photo-methionine and photo-leucine) and a covalent fixation of interaction partners. The captured protei...
ORGANISM(S): Homo sapiens (Human) 
2017-04-10 | PXD005349 | Pride
Pkd2 Affinity enrichment Protein interaction Photo-cross-linking Photo-methionine Photo-leucine Orbitrap fusion 
ProteomicsPhosphoproteomics combined with quantitative 14-3-3-affinity capture identifies SIRT1 and RAI as new regulators of cytosolic dsRNA-induced innate immune response
In this study, we have used global phosphoproteomic analysis to define phosphorylation-dependent signaling events regulated during RLR stimulation in human keratinocytes. In addition, we used quantitative 14-3-3-affinity capture to identify major target molecules of 14-3-3 regulation.
ORGANISM(S): Homo sapiens (Human) 
2014-07-08 | PXD000670 | Pride
Lc-ms\ms Phosphorylation Human keratinocytes 
TranscriptomicsAgo-APP: a GW peptide-based Argonaute affinity capture strategy
During microRNA (miRNA)-guided gene silencing, Argonaute (Ago) proteins interact with a member of the TNRC6/GW protein family. Here we used a short GW protein-derived peptide fused to GST and demonstrate that it binds to Ago proteins with high affinity. This allows for the simultaneous isolation of ...
ORGANISM(S): Arabidopsis thaliana 
2015-09-08 | E-GEOD-70553 | biostudies-arrayexpress
ProteomicsCAPTURE of the human U2 snRNA genes
In order to identify factors involved in transcription of human snRNA genes and 3’ end processing of the transcripts, we have carried out CRISPR affinity purification in situ of regulatory elements (CAPTURE), which is deadCas9-mediated pull-down, of the tandemly-repeated U2 snRNA genes in human cell...
ORGANISM(S): Homo sapiens (Human) 
2022-06-09 | PXD033580 | Pride
Transcription Snrna Interactome 
ProteomicsAPPLE-MS: A modular affinity purification-mass spectrometry method assisted by PafA- mediated proximity labeling
While affinity purification-mass spectrometry (AP-MS) has significantly advanced protein-protein interaction (PPI) studies, its limitations in detecting weak, transient, and membrane-associated interactions remain. To address these challenges, we introduced an innovative proteomic method termed Affi...
ORGANISM(S): Homo sapiens (Human) Rattus norvegicus (Rat) 
2025-10-27 | PXD063145 | Pride
Ap-ms Proximity labeling Protein-protein interaction Interactome 
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