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This study addresses this gap by conducting a direct comparison of eight platforms, representing both affinity-based and diverse mass spectrometry approaches, and covering over 13,000 proteins. By applying these platforms to the same cohort, we systematically assess their performance, identifying ke...
ORGANISM(S): Homo sapiens (Human) 
2025-11-06 | PAD000022 | Pride
HeLa cells were incubated with affinity-based probes, synthetic analogs of endogenous metabolites: putrescine, spermidine, and spermine. Cells were photoirradiated briefly and proteins were extracted, digested with trypsin, and the resulting peptides were labeled with TMT. Proteins were quantified a...
ORGANISM(S): Homo sapiens (Human) 
2025-09-22 | PXD062452 | Pride
Comprehensive interactome analysis of targeted proteins is of great significance to understand how pro-teins work together for functional regulation. Until now, affinity purification strategy with mass spec-trometry (AP-MS) has been recognized as the most widely used technique for studying protein-p...
ORGANISM(S): Homo Sapiens 
2022-12-01 | PXD038473 |
HeLa cells were incubated with affinity-based probes designed as synthetic analogs of endogenous metabolites: putrescine, spermidine, and spermine, while a monoamine compound served as a control. Probe-protein interactions were captured in situ using UV-induced crosslinking. After cell lysis, biorth...
ORGANISM(S): Homo sapiens (Human) 
2025-09-22 | PXD058706 | Pride
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