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ProteomicsCapillary zone electrophoresis-tandem mass spectrometry as an alternative to liquid chromatography-tandem mass spectrometry for top-down proteomics of histones with the identification of over 1000 histone proteoforms
Top-down proteomics (TDP) is an ideal approach for deciphering the histone code and it routinely employs reversed-phase liquid chromatography (RPLC)-tandem mass spectrometry (MS/MS). Here we present capillary zone electrophoresis (CZE)-MS/MS via the electro-kinetically pumped sheath-flow CE-MS inter...
ORGANISM(S): Bos taurus (Bovine) 
2022-04-04 | PXD021889 | Pride
Intact histone proteoforms/mobility prediction/top-down proteomics/ histone postlational modifications/calf histone 
ProteomicsAn improved capillary isoelectric focusing-mass spectrometry method for characterization of monoclonal antibody charge variants
Here, we improved the stability and enhanced separation resolution of automated cIEF-MS by bettering the quality of capillary neutral coating, reducing catholyte pH to 10 for cIEF-MS for the first time, and systematically optimizing the cIEF separation conditions for characterization of monoclonal a...
ORGANISM(S): Homo sapiens (Human) 
2026-08-19 | PXD082799 | Pride
Monoclonal antibody charge variants Cief-ms 
ProteomicsAn improved nanoflow RPLC-CZE-MS/MS system with high peak capacity and sensitivity for nanogram bottom-up proteomics
Novel mass spectrometry (MS)-based proteomic tools with extremely high sensitivity and high peak capacity are required for comprehensive characterization of protein molecules in mass-limited proteome samples. We previously reported a nanoflow RPLC-CZE-MS/MS (nanoRPLC-CZE-MS/MS) system for deep botto...
ORGANISM(S): Homo sapiens (Human) 
2019-10-23 | PXD014392 | Pride
Human cells Nanorplc-cze-ms/ms Bottom-up proteomics Nanograms 
ProteomicsMicro-scale RPLC-CZE-MS/MS for deep and highly sensitive bottom-up proteomics
Capillary zone electrophoresis-tandem mass spectrometry (CZE-MS/MS) has been well recognized for bottom-up proteomics. It has approached 4000-8000 protein identifications (IDs) from a human cell line, mouse brains or Xenopus embryos via coupling with liquid chromatography (LC) prefractionation. Howe...
ORGANISM(S): Homo sapiens (Human) 
2018-08-16 | PXD009991 | Pride
Micro-scale rplc Cze-ms/ms 
ProteomicsQuantitative proteomics after spinal cord injury in a regenerative and a non-regenerative stage in Xenopus laevis
The capacity to regenerate the spinal cord after an injury is a coveted trait that only a limited group of non-mammalian organisms can achieve. In Xenopus laevis, this capacity is only present during larval or tadpole stages, but is absent during postmetamorphic frog stages. This provides an excelle...
ORGANISM(S): Xenopus laevis (African clawed frog) 
2018-01-25 | PXD006993 | Pride
Spinal cord injury Regenerative and a non-regenerative stage Quantitative proteomics Xenopus laevis 
ProteomicsFinding the optimal degassing condition for linear polyacrylamide coated capillaries used in capillary zone electrophoresis-mass spectrometry-based top-down proteomics
LPA-coated capillaries are widely used; however, the quality of the coating—which directly affects separation performance and analyte identification—can vary significantly depending on the individual performing the procedure. A critical factor in achieving consistent coating quality is the degassing...
ORGANISM(S): Homo sapiens (Human) 
2026-05-14 | PXD067278 | Pride
Degass Polyacrylamide-coated capillary Top-down proteomics Reproducibility Capillary zone electrophoresis 
ProteomicsSingle-shot top-down proteomics with capillary zone electrophoresis-electrospray ionization-tandem mass spectrometry for identification of nearly 600 Escherichia coli proteoforms
Capillary zone electrophoresis-electrospray ionization-tandem mass spectrometry (CZE-ESI-MS/MS) has been recognized as an invaluable platform for top-down proteomics. However, the scale of top-down proteomics from CZE-MS/MS is still limited due to the low loading capacity and narrow separation windo...
ORGANISM(S): Escherichia coli 
2017-10-30 | PXD007273 | Pride
Top-down proteomics Cze-ms/ms E.coli 
ProteomicsA simple and efficient approach for preparing cationic coating with tunable electroosmotic flow for capillary zone electrophoresis-mass spectrometry-based top-down proteomics
Capillary zone electrophoresis-tandem mass spectrometry (CZE-MS/MS) has become a valuable analytical technique in top-down proteomics (TDP) in recent years. CZE-MS/MS-based TDP typically employs separation capillaries with neutral coatings (i.e., linear polyacrylamide, LPA). However, issues related ...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2024-10-15 | PXD051862 | Pride
Top-down proteomics  proteoform  capillary zone electrophoresis-mass spectrometry  covalent cationic coating  reproducibility  large proteoforms 
UnknownBacillus velezensis FiA2 as an Oxydifficidin-Producing Strain and its Effects on the Growth Performance, Immunity, Intestinal Microbiota, and Resistance to Aeromonas salmonicida Infection in Carassius carassius.
Not available
S-EPMC12532693 | biostudies-literature
ProteomicsMass spectrometry-based top-down proteomics in nanomedicine: proteoform-specific measurement of protein corona
Conventional mass spectrometry (MS)-based bottom-up proteomics (BUP) analysis of protein corona [i.e., an evolving layer of biomolecules, mostly proteins, formed on the surface of nanoparticles (NPs) during their interactions with biomolecular fluids] enabled nanomedicine community to partly identif...
ORGANISM(S): Homo sapiens (Human) 
2024-10-15 | PXD050779 | Pride
Protein corona Top-down proteomics Nanomedicine Plasma Proteoform 
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