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ProteomicsID8 cells manifest phenotypic plasticity and molecular heterogeneity of high-grade serous ovarian cancer in response to the local tissue niche ID8 cells manifest phenotypic plasticity and molecular heterogeneity of high-grade serous ovarian cancer in response to the local tissue niche
Spontaneously transformed mouse ovarian surface epithelial cell line ID8 generates a syngeneic mouse model, frequently used in pre-clinical evaluation. Towards a precise, subtype-specific perspective of such applications, we thought it pertinent to study its stratification into a specific molecular ...
ORGANISM(S): Mus musculus (Mouse) 
2025-10-13 | PXD060359 | Pride
Phenotypic plasticity D8 Ascites. 
ProteomicsHYDIN2 knockdown in NT2 cells analyses by LFQ
Segmental duplications at proximal loci can generate transcriptionally active chimeric pseudogenes (φgenes) by fusing contiguous sequences from two parental genes. These chimeric φgenes often co-opt 5′ regulatory elements for activation and acquire 3′ modifications that enhance transcript stability,...
ORGANISM(S): Homo sapiens (Human) 
2026-05-25 | PXD074698 | Pride
Hydin2 Chimeric pseudogene Lfq proteomics 
ProteomicsIP-MS Profiling of ANAPC1 and MP1 Interaction Networks in OVCAR3 Cells
We identified a pseudogene-derived chimeric transcript, vA-202, which encodes a partial ANAPC1-derived protein (MP1). To characterize its protein interaction network, we transiently expressed FLAG-tagged MP1 in OVCAR3 cells and performed immunoprecipitation using an anti-FLAG antibody. For compariso...
ORGANISM(S): Homo sapiens (Human) 
2026-05-25 | PXD070186 | Pride
Human Mp1 Anapc1 Ip-ms Ovarian cancer 
ProteomicsRNA pull down experiment for vA-202 (ANAPC1P2) lncRNA
This study aimed to identify protein interaction partners of the lncRNA vA-202 using an RNA pull-down approach followed by mass spectrometry. Biotinylated vA-202 RNA was generated by in vitro transcription incorporating biotin-14-CTP. For each pull-down reaction, 400 µg of whole-cell lysate (in a to...
ORGANISM(S): Homo sapiens (Human) 
2026-05-25 | PXD070150 | Pride
Human Rna pull down Orbutrap ms 
ProteomicsOrbitrap proteomic analysis of five distinct molecular subtypes of High Grade Serous Ovarian Carcinoma (HGSOC) under serum deprived and serum fed conditions
Cell lines representing five distinct phenotypes of high-grade serous ovarian carcinoma (HGSOC), including Epithelial (E), Intermediate-Epithelial (iE), Epithelial-Mesenchymal Hybrid (E/M), Intermediate-Mesenchymal (iM), and Mesenchymal (M), previously classified in our laboratory based on the expre...
ORGANISM(S): Homo sapiens (Human) 
2025-10-08 | PXD062888 | Pride
Serum starvation A4 Caov3 Ovarian cancer Ovcar3 High grade serous ovarian carcinoma (hgsoc) Ovca420 Ovmz6 
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