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ProteomicsEvaluation of a bead-free immunoprecipitation technique coupled with tandem mass spectrometry for identification of plant-virus protein interactions (DeBlasio_JBT_2017)
Potato leafroll virus (PLRV) is an aphid-borne, RNA virus in the Luteoviridae that causes significant loss to potato production worldwide. Precision methods to block virus infection in plants or the transmission of a virus by the insect represent new approaches to virus disease management and requir...
ORGANISM(S): Nicotiana benthamiana 
2018-10-24 | PXD006338 | Pride
Host-pathogen interactions Bead-free immunoprecipitation  plant-virus interactome  tandem mass spectrometry Luteoviridae Polerovirus Molecular virology Potato leafroll virus Phloem-limited 
TranscriptomicsRNA m5C Methylation in breast cancer using MeRIP-Seq
RNA m5C methylation profile of MCF10A and MDA486 by using MeRIP-Seq protocol Immunoprecipitation of Methylated mRNA at Cytosine (m5C) residues: Affinity purified of anti-methyl cytosine (m5C) polyclonal antibody 7ug (Zymo Research, Catalog#A3001-50) was conjugated with protein-A magnetic beads for 2...
ORGANISM(S): Homo sapiens 
2014-02-20 | E-GEOD-53370 | biostudies-arrayexpress
TranscriptomicsMassive sequencing of hybrid DNA obtained by DNA:RNA immunoprecipitation (DRIP) in extracellular microvesicles (MV) produced by HeLa cells (using S9.6 antibody)
Hybrid (Bound) and non-hybrid (Unbound) DNA from Hela EV was isolated using DRIP procedure as follows. Between 1-5μg of nucleic acid DNA from EVs (DNase0 treated) was incubated overnight with 1μg of anti-DNA-RNA Hybrid (S9.6) antibody (KeraFast) under rotation at 4°C in 500μl of binding buffer (10mM...
ORGANISM(S): Homo sapiens 
2023-03-30 | E-MTAB-9634 | biostudies-arrayexpress
ProteomicsTent5a Immunoprecipitation in pancreatic beta cells
Wild Type Tent5a or catalytically inactive Tent5a was over-expressed and immunoprecipitated from INS-1E cell lysates. Binding partners were identified using LC-MS.
ORGANISM(S): Rattus norvegicus (Rat) 
2025-08-22 | PXD066929 | Pride
Lc-ms Immunoprecipitation Tent5a Pancreatic beta cells 
ProteomicsRegulation of the HTRA2 protease activity by PDZ domain-binding peptide ligands and the influence on HTRA2-specific protein interaction networks in retinal tissues
For the identification of HTRA2-specific protein interaction partners in retinal tissues of the house swine (Sus scrofa), we performed co-immunoprecipitation experiments with recombinant 6His-tag HTRA2 followed by quantitative LC-MS-based proteomics (see Fig. 2A and File S1). In brief, 2 µg recombin...
ORGANISM(S): Homo sapiens (Human) Sus scrofa domesticus (domestic pig) 
2021-10-11 | PXD024442 | Pride
Htra2  neuroprotection  retina  interaction partners  co-immunoprecipitation  mass spectrometry 
ProteomicsThe protein interactomes of AtNHR2A and AtNHR2B unraveled common and specialized functions in plant immunity integrating distinct biological processes
AtNHR2A (Arabidopsis thaliana nonhost resistance 2A) and AtNHR2B (Arabidopsis thaliana nonhost resistance 2B) are two proteins required in nonhost resistance, a broad spectrum mechanism of plant immunity that protects plants against pathogens. AtNHR2A and AtNHR2B are localized to the cytoplasm, chlo...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2020-03-10 | PXD015894 | Pride
Affinity purification Arabidopsis Mass spectrometry Co-immunoprecipitation Plant immunity 
TranscriptomicsRNA expression in tongue squamous cell carcinoma, tumour free tongue tissue and control tongue
Tongue squamous cell carcinoma is a tumour type with rather low five year survival, around 60%. The poor survival rate has been ascribed to late detection, a high frequency of locoregional recurrence, the occurrence of secondary primary tumours and death due to comorbidity. One reason for developmen...
ORGANISM(S): Homo sapiens 
2016-12-21 | E-MTAB-4678 | biostudies-arrayexpress
ProteomicsIdentification of phosphorylation sites in human IGF2BP1 by immunoprecipitation coupled to mass spectrometry
Many RNA-binding proteins are regulated by posttranslational modifications, most commonly phosphorylation. IGF2BP1 is an RNA-binding protein, which regulates protein stability, translation and localization. Moreover, it was proposed to regulate mRNA stability and translation during proteotoxic stres...
ORGANISM(S): Homo sapiens (Human) 
2024-11-13 | PXD045761 | Pride
Q exactive hf-x Human Igf2bp1 Proteotoxic stress Hek293t Igf2bp3 
GenomicsMassive sequencing of hybrid DNA obtained by DNA:RNA immunoprecipitation (DRIP) in extracellular microvesicles (MV) produced by HeLa cells (using S9.6 antibody)
Massive sequencing of hybrid DNA obtained by DNA:RNA immunoprecipitation (DRIP) in extracellular microvesicles (MV) produced by HeLa cells (using S9.6 antibody)
PRJEB40619 | ENA
ProteomicsMass spectrometry of co-immunoprecipitation using tobacco Maturase K as bait
Chloroplast group IIA introns derive from bacterial ribozymes. Their splicing likely requires Maturase K (MatK), which has been largely inaccessible to functional analyses being itself a chloroplast intron-encoded protein. Here we used co-immunoprecipitation coupled with proteomics to identify prote...
ORGANISM(S): Nicotiana tabacum (Common tobacco) 
2026-02-10 | PXD060055 | Pride
Lc-ms/ms Tobacco Co-immunoprecipitation Seedilings Chloroplasts 
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