Sort   by:  
 Page size 
ProteomicsDeciphering O-glycoprotease substrate preferences with O-Pair Search
O-glycoproteases are an emerging class of enzymes that selectively digest glycoproteins at positions decorated with specific O-linked glycans. O-glycoprotease substrates range from any O-glycoprotein (albeit with specific O-glycan modifications) to only glycoproteins harboring specific O-glycosylate...
ORGANISM(S): Homo sapiens (Human) 
2023-02-15 | PXD035775 | Pride
O-glycoproteomics Glycoproteins Cleavage Glycoproteomics Informatics O-pair search O-glycoprotease 
ProteomicsElectron-based dissociation is needed for O-glycopeptides derived from OpeRATOR proteolysis
The recently described O-glycoprotease OpeRATOR presents exciting opportunities for O-glycoproteomics. This bacterial enzyme purified from A. muciniphila cleaves N-terminally to serine and threonine residues that are modified with (preferably asialylated) O-glycans, providing orthogonal cleavage rel...
ORGANISM(S): Bos taurus (Bovine) Homo sapiens (Human) 
2020-11-03 | PXD020077 | Pride
Glycoproteomics O-glycosites Operator Protease Electron transfer dissociation 
ProteomicsMutational screens highlight glycosylation as a modulator of colony-stimulating factor 3 receptor (CSF3R) activity
The colony-stimulating factor 3 receptor (CSF3R) controls the growth of neutrophils, the most abundant type of white blood cell. In healthy neutrophils, signaling is dependent on CSF3R binding to its ligand, CSF3. A single amino acid mutation in CSF3R, T618I, instead allows for constitutive, ligand-...
ORGANISM(S): Homo sapiens (Human) 
2023-07-20 | PXD035664 | Pride
Receptor Mutagenesis Neutrophil Glycoprotein 
ProteomicsDiscovery of a pathway for endogenous mucin glycodomain catabolism in mammals
Mucins are functionally implicated in a range of human pathologies, including cystic fibrosis, influenza, bacterial endocarditis, gut dysbiosis, and cancer. These observations have motivated the study of mucin biosynthesis as well as development of strategies for inhibition of mucin glycosylation. M...
ORGANISM(S): Homo sapiens (Human) Mus musculus (Mouse) 
2022-09-03 | PXD027616 | Pride
Human Catabolism Glycoproteomics Mucins Mucinase 
ProteomicsThe modified RNA base acp3U is an attachment site for N-glycans in glycoRNA
GlycoRNA consists of RNAs modified with secretory N-glycans that are presented on the cell surface. While previous work supported a covalent linkage between RNA and glycans, the direct chemical nature of the RNA-glycan connection was not described. Here we develop a sensitive and scalable protocol t...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2023-10-31 | MSV000093213 | MassIVE
Sort   by:  
 Page size