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ProteomicsA ubiquitin-specific, proximity-based labeling approach for the identification of ubiquitin ligase substrates
Ubiquitination of proteins is central to protein homeostasis and other cellular processes including DNA repair, vesicular transport, cell-division etc. The process of ubiquitination is conserved from yeast to humans and is carried out by the sequential action of three enzymes: E1, E2 and E3. There ...
ORGANISM(S): Homo sapiens (Human) 
2024-11-26 | PXD045521 | Pride
Bira Substrate identification U box Ring E3 ligase Ubiquitin 
ProteomicsComparative_Interaction_Proteomics_GFP-tagged_MAGEC2_HEK293T
293T (Human embryonic kidney cells-ATCC CRL-3216) cells were plated at 500,000 cells per 10 cm dish and grown for 48 hours. Cells were then transfected with GFP-MAGEC1 WT or GFP-MAGEC1 L318D in a 3:1 ratio of PEI (polyethylenimine) to DNA. Cells were harvested after 48 hours and lysed in 50 mM Tris...
ORGANISM(S): Homo sapiens (Human) 
2024-02-20 | PXD047849 | Pride
Hek293t cells Magec2 Proteomics 
ProteomicsStructural basis for the inhibition of pathogenic serine ubiquitination by the Legionella metaeffector SidJ
Structural and functional studies of SidE-family effectors and the metaeffector SidJ in the infection mechanism of Legionella pneumophila, elucidating the role of post-translational modification in modulating effector toxicity.
ORGANISM(S): Legionella pneumophila 
2021-09-20 | PXD028638 | Pride
Serine ubiquitination Autoampylation Legionella Glutamylation Adp-ribosylation Adenylation Ubiquitin 
ProteomicsDiGly_Analysis_RAD18_RAD6_with/without_MAGEA4
Auto-ubiquitination assay performed in which the E3 ligase complex RAD18-RAD6 was incubated with E1 and ubiquitin in the presence/absence of MAGEA4for 1 hour at 37C. All proteins were purified from E.coli. Samples were loaded and run onto a precast SDS PAGE gel, which was stained with Coomasie. Appr...
ORGANISM(S): Homo sapiens (Human) 
2024-02-20 | PXD047850 | Pride
Digly Rad18 Ubiquitination Magea4 Rad6 
ProteomicsRAD18_RAD6_MAGEA4_Cross-linking_Mass_Spectrometry
RAD18 and RAD6 were co-expressed in E.coli, RAD18 harbors an N-terminal 6His tag which was utilized for affinity chromatography via cobalt affinity resin. The complex was then subjected to size exclusion chromatography. 5 X molar excess MAGEA4 (also has N-terminal His-tag) was added to RAD18-RAD6. T...
ORGANISM(S): Homo sapiens (Human) 
2024-02-20 | PXD047929 | Pride
Magea4 Xlms Rad6 Rad18 
ProteomicsStructural basis for the toxicity of Legionella pneumophila effector SidH
Legionella pneumophila (LP) secretes more than 300 effectors into the host cytosol to facilitate intracellular replication. One of these effectors, SidH does not have sequence similarityto proteins of known function and is toxic when overexpressed in host cells. In order to understand the mechanism ...
ORGANISM(S): Homo sapiens (Human) 
2023-10-02 | PXD044950 | Pride
Sidh Lc-ms Interactors Legionella pneumophila 
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