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GenomicsRNA-seq of biotin-labelled-miR190b pulldown
RNA-seq of biotin-labelled-miR190b pulldown
PRJNA816541 | ENA
ProteomicsRNA pulldown-MS, Dlg4 RNA in mouse brain
Goal was to identify RNA-binding proteins with a preference for m6A-modified RNA using an RNA bait generated from a known m6A site in the Dlg4 (PSD-95) mRNA 3`-UTR. By comparison of differential enrichment between m6A-RNA pulldown fractions and A-RNA or no RNA fractions, we can identify m6A-binding ...
ORGANISM(S): Mus musculus (Mouse) 
2022-10-13 | PXD034159 | Pride
Pulldown M6a Rna Lc-ms/ms 
ProteomicstRF-24 RNA pulldown in KYSE150 and KYSE30
To delineate the oncogenic mechanism of tRF-24 in ESCC, we performed RNA pull-down assays with biotinylated tRF-24 and antisense control, followed by mass spectrometry (MS) analysis.
ORGANISM(S): Homo sapiens (Human) 
2025-12-01 | PXD063163 | Pride
Human Kyse30 Esophagus Rna-pulldown Kyse150 
ProteomicsRNA pulldown-MS, Dlg4 RNA in mHippoE-2 cells
Goal was to identify RNA-binding proteins with a preference for m6A-modified RNA using an RNA bait generated from a known m6A site in the Dlg4 (PSD-95) mRNA 3`-UTR. By comparison of differential enrichment between m6A-RNA pulldown fractions and A-RNA or no RNA fractions, we can identify m6A-binding ...
ORGANISM(S): Mus musculus (Mouse) 
2022-10-13 | PXD034161 | Pride
Pulldown M6a Rna Lc-ms/ms 
ProteomicsPulldown of RNA binding protein using SARS CoV 2 NSP1 RNA
RNA sensing is critical for RLR activation and downstream signaling. To identify molecules that are involved in modulating viral RNA recognition, we utilized biotin-labeled 60 nt single-stranded RNA (ssRNA) from SARS-CoV-2 NSP1 coding region (NSP1 ssRNA) to pull down proteins from RAW264.7 cells. Th...
ORGANISM(S): Mus musculus (Mouse) 
2026-04-08 | PXD064808 | Pride
Pulldown  rna binding protein  nsp1 rna  sars-cov-2 
Proteomicsbiotin RNA pulldown assay of lnc-GD2H and mass spectrometry
Vectors carrying lnc-GD2H and antisense lnc-GD2H were linearized with the corresponding restriction enzymes to prepare template DNAs for in vitro transcription. Biotinylated RNAs were mixed with proteins extracted from C2C12 cells, followed by targeting of the RNAs with streptavidin beads. After SDS...
ORGANISM(S): Mus musculus (Mouse) 
2021-05-18 | PXD025292 | Pride
C2c12 Myoblast Biotin rna pulldown Ms Mice Lnc-gd2h 
ProteomicsIdentification of proteins specifically binding to ac4C RNA probes
As a newly identified mRNA modification, the regulation of ac4C remains largely unexplored. RNA-binding proteins (RBPs) that specifically binds to ac4C modification and mediate downstream cellular activities (readers) have not been reported yet. We synthesized acetylated and non-acetylated RNA probe...
ORGANISM(S): Homo sapiens (Human) 
2021-07-20 | PXD027014 | Pride
Rna pulldown Ac4c Acetylated rna probes 
ProteomicsBiotin-pgRNAWT or Biotin-pgRNAAUUUA to AUCUC RNA pulldown LC-MS/MS
The nuclear export of HBV RNAs allows the virus to synthesize its proteins through the translation machinery and replicate its genome through reverse transcription in the cytoplasm. However, the molecular mechanisms underlying this important process remain largely obscure. To illustrate this process...
ORGANISM(S): Homo sapiens (Human) 
2024-05-21 | PXD044015 | Pride
Rna nuclear export Pgrna binding proteins Rna stability Au rich element Hbv rna 
TranscriptomicsNovel RNA-binding activity of MYF5 enhances Ccnd1/Cyclin D1 mRNA translation during myogenesis
Skeletal muscle contains long multinucleated and contractile structures known as muscle fibers, which arise from the fusion of myoblasts into nucleated myotubes during myogenesis. The myogenic regulatory factor (MRF) MYF5 is the earliest to be expressed during myogenesis and functions as a transcri...
ORGANISM(S): Mus musculus 
2016-08-08 | E-GEOD-73704 | biostudies-arrayexpress
TranscriptomicsGlobal Analysis of Glucocorticoid Receptor-Associated Transcripts and Identification of a Target RNA Motif
Posttranscriptional regulation is emerging as a key factor in glucocorticoid (GC)-mediated gene regulation. We investigated the role of the human glucocorticoid receptor (GR) as an RNA-binding protein and its effect on mRNA turnover in human airway epithelial cells. Cell treatment with the potent ...
ORGANISM(S): Homo sapiens 
2011-06-24 | E-GEOD-24748 | biostudies-arrayexpress
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