Sort   by:  
 Page size 
TranscriptomicsaCGH analyses of DSB-induced LOH in Blm-deficient mESCs
Genome modification in mouse embryonic stem cells (mESCs) has provided the versatile platform to analyzing gene functions in mammalian cells. Bloom helicase-deficient mESCs showed elevated rate of loss of heterozygosity (LOH) through mitotic recombination. By utilizing this characteristic of Blm de...
ORGANISM(S): Mus musculus 
2012-11-30 | E-MTAB-1116 | biostudies-arrayexpress
ProteomicsRole of BLM helicase in augmenting the phosphorylation of DNA damage response (DDR) proteins.
Assessing the importance of BLM helicase upon DNA damage in the phosphorylation enhancement of DNA damage response (DDR) proteins via phosphoproteomic profiling in the presence and absence of BLM.
ORGANISM(S): Mus musculus (Mouse) 
2025-05-06 | PXD048117 | Pride
Dna repair Atm Dna damage response Blm helicase 
ProteomicsBLM helicase protein negatively regulates stress granule formation through unwinding RNA G-quadruplex structures
Bloom’s syndrome (BLM) protein is a known nuclear helicase that is able to unwind DNA secondary structures such as G-quadruplexes. However, its role in the regulation of cytoplasmic processes that involve RNA G-quadruplexes (rG4s) has not been previously studied. Here, we demonstrate that BLM is rec...
ORGANISM(S): Homo sapiens (Human) 
2023-10-24 | PXD039534 | Pride
Stress granule Apex Helicase Lc-msms G-quadruplex Rg4-binding proteins Blm Quma-1 
ProteomicsBLM complex composition in unperturbed cells
The Bloom syndrome DNA helicase BLM contributes to chromosome stability through its roles in double-strand break repair by homologous recombination and DNA replication fork restart during the replication stress response. Loss of BLM activity leads to Bloom syndrome, which is characterized by extraor...
ORGANISM(S): Homo sapiens (Human) 
2022-02-15 | PXD018823 | Pride
Gm00637 Q exactive plus Human 
ProteomicsBLM helicase protein negatively regulates stress granule formation through unwinding RNA G-quadruplex structures
We ran native 5% polyacrylamide-TBE gel for electro mobility shift assay (EMSA) for DNA/RNA G4 structures with or without recombinant human core-BLM (cBLM) protein, which was extracted and purified from E.coli in order to show binding of the RNA/DNA by this protein. To further evidence, we cut the e...
ORGANISM(S): Homo sapiens (Human) 
2023-10-24 | PXD042983 | Pride
Blm Sg Emsa G-quadruplex Lc-ms/ms 
ProteomicsThe Bloom’s syndrome helicase BLM functions as a homodimer within the Bloom’s complex
Homologous recombination (HR) is an essential and highly regulated cellular process. Unintended HR in somatic cells is potentially deleterious; it can result in translocations and/or somatic cell loss of heterozygosity. This is suppressed by the BLM protein in combination with topoisomerase III, RM...
ORGANISM(S): Homo sapiens (Human) 
2022-01-17 | PXD011078 | Pride
Blm Crosslinking ms Structural proteomics Bloom’s syndrome 
GenomicsA POLD3/BLM dependent pathway handles DSBs in transcribed chromatin upon excessive RNA:DNA hybrids accumulation
Transcriptionally active loci are particularly prone to breakage and mounting evidence suggest that DNA Double-Strand Breaks arising in active genes are handled by a dedicated repair pathway, Transcription-Coupled DSB Repair (TC-DSBR), that entails R-loop accumulation and dissolution. Here, we uncov...
ORGANISM(S): Homo sapiens 
2022-04-13 | E-MTAB-11592 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of S. cerevisiae sgs1 mutant and wild type strains grown under DNA damaging and control conditions
Wild type and sgs1 null yeast were grown under DNA damaging (with MMS) conditions or without treatment to log phase and their transcriptional profiles compared. The human aging diseases Werner and Bloom syndromes are a result of mutation of the WRN and BLM genes, respectively. The SGS1 gene of Sacch...
ORGANISM(S): Saccharomyces cerevisiae 
2007-07-07 | E-GEOD-423 | biostudies-arrayexpress
UnknownhSSB1 associates with and promotes stability of the BLM helicase.
Not available
S-EPMC5433028 | biostudies-literature
UnknownBLM helicase facilitates telomere replication during leading strand synthesis of telomeres.
Not available
S-EPMC4508891 | biostudies-literature
Sort   by:  
 Page size