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ProteomicsBranched-K29/K48 and K48/K29 polyUb_LC-MSMS
The E3 ubiquitin ligase Ufd4/TRIP12 and the E4 enzyme Ufd2 are capable of catalyzing branched ubiquitination. Here, we employ LC-MS/MS to characterize the specific ubiquitin linkage types involved in these branched ubiquitin chains.
ORGANISM(S): Homo sapiens (Human) Saccharomyces cerevisiae (Baker's yeast) 
2025-04-10 | PXD062799 | Pride
Branched ubiquitin chain lc-msms 
ProteomicsThe UBA domain of Ubc1 (Ube2K) Facilitates the Assembly of K48/K63-Branched Ubiquitin Chains
The assembly of a specific polymeric ubiquitin (Ub) chain on a target protein is a key event in the regulation of numerous cellular processes. Yet, the mechanisms that govern the selective synthesis of particular polyubiquitin signals remain enigmatic. The ubiquitin-conjugating (E2) enzymes Ubc1 in ...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2021-03-02 | PXD022621 | Pride
Ubiquitin Ubc1 Ubc4 Yeast Branched 
ProteomicsUbiREAD deciphers proteasomal degradation code of homotypic and branched K48 and K63 ubiquitin chains
Ubiquitin chains encode signals defining the fates of their modified proteins. Ubiquitin chain-mediated proteasomal degradation is a widespread eukaryotic regulatory mechanism. Yet, heterogeneity of intracellular ubiquitination has precluded systematically comparing degradation capacities of differe...
ORGANISM(S): Homo sapiens (Human) 
2025-06-23 | PXD060731 | Pride
Ubiquitin K48 Deubiquitination K63 Protein degradation Proteasome Proteostasis Electroporation Ubiquitin code Branched ubiquitin chains 
ProteomicsThe UBA-domain of Ubc1/Ube2K Facilitates the Assembly of K48-/K63-Branched Ubiquitin Chains
Assembly of polymeric ubiquitin (Ub) chains is an essential posttranslational protein modification that regulates widespread intracellular processes in eukaryotic cells. Factors and mechanisms that regulate the formation of Ub chains are key to the understanding of many cellular processes. The E2 en...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2021-09-09 | PXD018651 | Pride
Ubc1/ube2k ubiquitin k48-/k63-branched ubiquitin prm 
ProteomicsK48-K63-Ubiquitin chain pulldown from U2OS cell lysates
SpyTagged branched and unbranched K48- and K63-linked Tetra-Ubiquitin chains were immobilized on SpyCatcher agarose (48Ub4; 63Ub4; [Ub]2-48,63Ub-48Ub; [Ub]2-48,63Ub-63Ub). Pulldown were performed from protease-inhibitor treated U2OS cell lines to identify specific binders of K48-K63-branched Ub4.
ORGANISM(S): Homo sapiens (Human) 
2024-05-07 | PXD046025 | Pride
Human Lc-msms Dia Ubiquitin Branched ubiquitin 
ProteomicsK48- and K63-linked ubiquitin chain interactome reveals branch- and length-specific ubiquitin interactors
Ubiquitin-interactor co-pulldown coupled with mass spectrometry used to elucidate K48- and K63-linked interactomes including those of Ub chains of varying length (Ub-Ub3) and heterotypic branched Ub chains. Ubiquitin-interactors were enriched from human HeLa and yeast s. cerevisiae cell lysate with ...
ORGANISM(S): Candida albicans 
2024-05-15 | PXD051890 | Pride
Branched ubiquitin chains / interactor / protein-protein interaction / proteomics / proteostasis / ubiquitin 
ProteomicsReprograming of Proteasomal Degradation by Branched Chain Amino Acid Metabolism
Branched chain amino acids (BCAAs) play an important role in energy and protein regulation. Defects in BCAA metabolism are linked to numerous pathologies, including diabetes, neurodegeneration, and premature aging. Isovaleric acidemia is a metabolic disease caused by defective leucine breakdown and ...
ORGANISM(S): Caenorhabditis elegans 
2022-09-22 | PXD028286 | Pride
C. elegans  ubiquitin  leucine catabolism  branched chain amino acid (bcaa)  bcat  ivd  isovaleric acidemia 
UnknownEmerging functions of branched ubiquitin chains.
Not available
S-EPMC7835216 | biostudies-literature
ProteomicsMARCH1 ubiquitination of MHC II generates a branched K63/K11 ubiquitin chain.
Major histocompatibility class II (MHC II) is critical for adaptive immunity. MHC II intracellular trafficking and degradation is regulated by ubiquitination, with poly-ubiquitination (Ub) of MHC II directing it away from the plasma membrane. The MHC II poly-Ub chain that dictates the fate and funct...
ORGANISM(S): Mus musculus (Mouse) 
2025-12-01 | PXD063057 | Pride
Ubiquitin Mhcii Ub-code Aqua peptides 
ProteomicsTwo DOT1 enzymes cooperatively mediate efficient ubiquitin-independent histone H3 lysine 76 tri-methylation in kinetoplastids
In higher eukaryotes, a single DOT1 histone H3 lysine 79 (H3K79) methyltransferase processively produces H3K79me2/me3 from me0 through histone H2B mono-ubiquitin interaction. The early-branched kinetoplastid Trypanosoma brucei harbors the essential H3K76 di-methyltransferase DOT1A and the non-essent...
ORGANISM(S): Trypanosoma Brucei (ncbitaxon:5691) 
2022-11-09 | MSV000090680 | MassIVE
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