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ProteomicsUbiREAD deciphers proteasomal degradation code of homotypic and branched K48 and K63 ubiquitin chains
Ubiquitin chains encode signals defining the fates of their modified proteins. Ubiquitin chain-mediated proteasomal degradation is a widespread eukaryotic regulatory mechanism. Yet, heterogeneity of intracellular ubiquitination has precluded systematically comparing degradation capacities of differe...
ORGANISM(S): Homo sapiens (Human) 
2025-06-23 | PXD060731 | Pride
Ubiquitin K48 Deubiquitination K63 Protein degradation Proteasome Proteostasis Electroporation Ubiquitin code Branched ubiquitin chains 
ProteomicsBranched-K29/K48 and K48/K29 polyUb_LC-MSMS
The E3 ubiquitin ligase Ufd4/TRIP12 and the E4 enzyme Ufd2 are capable of catalyzing branched ubiquitination. Here, we employ LC-MS/MS to characterize the specific ubiquitin linkage types involved in these branched ubiquitin chains.
ORGANISM(S): Homo sapiens (Human) Saccharomyces cerevisiae (Baker's yeast) 
2025-04-10 | PXD062799 | Pride
Branched ubiquitin chain lc-msms 
ProteomicsThe UBA-domain of Ubc1/Ube2K Facilitates the Assembly of K48-/K63-Branched Ubiquitin Chains
Assembly of polymeric ubiquitin (Ub) chains is an essential posttranslational protein modification that regulates widespread intracellular processes in eukaryotic cells. Factors and mechanisms that regulate the formation of Ub chains are key to the understanding of many cellular processes. The E2 en...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2021-09-09 | PXD018651 | Pride
Ubc1/ube2k ubiquitin k48-/k63-branched ubiquitin prm 
ProteomicsThe UBA domain of Ubc1 (Ube2K) Facilitates the Assembly of K48/K63-Branched Ubiquitin Chains
The assembly of a specific polymeric ubiquitin (Ub) chain on a target protein is a key event in the regulation of numerous cellular processes. Yet, the mechanisms that govern the selective synthesis of particular polyubiquitin signals remain enigmatic. The ubiquitin-conjugating (E2) enzymes Ubc1 in ...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2021-03-02 | PXD022621 | Pride
Ubiquitin Ubc1 Ubc4 Yeast Branched 
ProteomicsK48- and K63-linked ubiquitin chain interactome reveals branch- and length-specific ubiquitin interactors
Ubiquitin-interactor co-pulldown coupled with mass spectrometry used to elucidate K48- and K63-linked interactomes including those of Ub chains of varying length (Ub-Ub3) and heterotypic branched Ub chains. Ubiquitin-interactors were enriched from human HeLa and yeast s. cerevisiae cell lysate with ...
ORGANISM(S): Candida albicans 
2024-05-15 | PXD051890 | Pride
Branched ubiquitin chains / interactor / protein-protein interaction / proteomics / proteostasis / ubiquitin 
ProteomicsK48-K63-Ubiquitin chain pulldown from U2OS cell lysates
SpyTagged branched and unbranched K48- and K63-linked Tetra-Ubiquitin chains were immobilized on SpyCatcher agarose (48Ub4; 63Ub4; [Ub]2-48,63Ub-48Ub; [Ub]2-48,63Ub-63Ub). Pulldown were performed from protease-inhibitor treated U2OS cell lines to identify specific binders of K48-K63-branched Ub4.
ORGANISM(S): Homo sapiens (Human) 
2024-05-07 | PXD046025 | Pride
Human Lc-msms Dia Ubiquitin Branched ubiquitin 
UnknownEmerging functions of branched ubiquitin chains.
Not available
S-EPMC7835216 | biostudies-literature
UnknownEnhanced protein degradation by branched ubiquitin chains.
Not available
S-EPMC4028144 | biostudies-literature
UnknownK63 ubiquitylation triggers proteasomal degradation by seeding branched ubiquitin chains.
Not available
S-EPMC5816176 | biostudies-literature
UnknownUbiquitin Chain Enrichment Middle-Down Mass Spectrometry Enables Characterization of Branched Ubiquitin Chains in Cellulo.
Not available
S-EPMC5541364 | biostudies-literature
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