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ProteomicsBasic Strong Cation Exchange Chromatography, BaSCX, a Highly Efficient Approach for C-Terminomic Studies Using LysargiNase Digestion
Decoding protein C-termini is a challenging task in protein chemistry using conventional chemical/enzymatic approaches. With the rapid development in modern mass spectrometer, many advanced mass spectrometry (MS) based protein C-termini analysis approaches have been established. Although great progr...
ORGANISM(S): Homo sapiens (Human) Mus musculus (Mouse) Caenorhabditis elegans 
2020-02-28 | PXD016317 | Pride
C-terminomics / lysarginase / scx / bascx / mass spectrometry 
ProteomicsSAPT, a fast and efficient approach for simultaneous profiling of protein N- and C-terminome
Protein termini play pivotal roles in various biological processes. Although a series of terminomic strategies have been proposed for the analysis of protein N-termini or protein C-termini separately, few can analyze both ends of proteins at the same time. Herein, we developed a workflow, termed Sim...
ORGANISM(S): Homo sapiens (Human) 
2021-07-29 | PXD024573 | Pride
Human Protein termini Hela cell 
UnknownIn vivo N-Terminomics Highlights Novel Functions of ADAMTS2 and ADAMTS14 in Skin Collagen Matrix Building.
Not available
S-EPMC8017238 | biostudies-literature
UnknownOne-step N-Terminomics Based on Isolation of Protein N-Terminal Peptides From LysargiNase Digests by Tip-Based Strong Cation Exchange Chromatography.
Not available
S-EPMC11382313 | biostudies-literature
UnknownPBC, an easy and efficient strategy for high-throughput protein C-terminome profiling.
Not available
S-EPMC9471192 | biostudies-literature
ProteomicsCarboxypeptidase B-assisted charge-based fractional diagonal chromatography for deep screening of C-terminome
The determination of protein C-termini is of great significance for protein function annotation and proteolysis re-search. However, the progress of C-terminomics is still far behind its counterpart, N-terminomics, because of the low reactivity of the carboxyl group. Herein, we developed a negative s...
ORGANISM(S): Homo sapiens (Human) Escherichia coli 
2020-05-25 | PXD018520 | Pride
Chafradic Cleavage site C-terminomic Caspase 
ProteomicsHelical superstructures between amyloid and collagen VI in heart derived fibrils from a patient with Light Chain Amyloidosis
We investigated the N- and C-terminome of the LCs proteoforms in fibrils extracted from the hearts of a patient affected by AL amyloidosis, using a proteomic approach based on N- and C-terminal residues derivatization, followed by mapping of fragmentation sites on the structures of fibrillar LCs
ORGANISM(S): Homo sapiens (Human) 
2024-07-02 | PXD049301 | Pride
Amyloid fibrils  fragmentation sites  immunoglobulin light chains 
ProteomicsNAPT, an unbiased approach for sequential analysis of protein N- and C- terminome
Robust analysis of both protein N- and C-termini can reveal novel N- and C-degrons that modify protein stability and shape the eukaryotic proteome. Herein, we designed a workflow, termed LysargiNase-assisted Analysis of Protein N- and C-Terminome (NAPT) by sequential and selective separating N- and ...
ORGANISM(S): Homo Sapiens 
PXD030791 |
UnknownImpact of DJ-1 and Helix 8 on the Proteome and Degradome of Neuron-Like Cells.
Not available
S-EPMC7920061 | biostudies-literature
ProteomicsPBC, an easy and efficient strategy for high-throughput protein C-terminome profiling
So far, high-efficiency and high-throughput analysis of C-terminal peptides is still full of challenges. In order to make the chemical derivatization-based strategies more practical and efficient, in this study we developed a new method, named 2-pyridinecarboxaldehyde (2PCA) and biotin labeling bas...
ORGANISM(S): Homo Sapiens 
PXD035645 |
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