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ProteomicsArabidopsis thaliana N-terminal proteome,LC-MS
An extended ChaFRADIC workflow was applied to analyze the N-terminal proteome of Arabidopsis thaliana seedlings. Using iTRAQ protein labeling, a multi-enzyme digestion approach including trypsin, GluC, and subtilisin, a total of 200 μg per enzyme were used, and only 1/3 of each ChaFRADIC-enriched fr...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2015-07-21 | PXD001855 | Pride
Arabidopsis thaliana Chafradic Itraq reagent Enrichment of n-terminal peptide 
ProteomicsThe rhomboid protease PARL regulates apoptosis via proteolytic maturation of Smac/DIABLO in mitochondria
Substrates of the mitochondrial protease PARL were identified using quantitative N-terminal ChaFRADIC:
ORGANISM(S): Homo sapiens (Human) 
2017-03-09 | PXD005535 | Pride
Parl substrates Chafradic N-terminomics 
ProteomicsChaFRADIC: A novel SCX-based approach for N-terminal Enrichment
Step 1: Cell lysis of yeast sphaeroblast (Saccharomyces cerevisiae) and carbamidomethylation. Step 2: Isotopic labeling with dimethylation (light + medium). Step 3: Proteolytic digestion with trypsin (ArgC specificity because of blocked lysines by dimethylation). Step 4: Enrichment via ChaFRADIC...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2013-08-09 | PXD000292 | Pride
Chafradic Yeast Protease Peptidase Icp55 Cofradic N-terminal Degradomics 
ProteomicsCarboxypeptidase B-assisted charge-based fractional diagonal chromatography for deep screening of C-terminome
The determination of protein C-termini is of great significance for protein function annotation and proteolysis re-search. However, the progress of C-terminomics is still far behind its counterpart, N-terminomics, because of the low reactivity of the carboxyl group. Herein, we developed a negative s...
ORGANISM(S): Homo sapiens (Human) Escherichia coli 
2020-05-25 | PXD018520 | Pride
Chafradic Cleavage site C-terminomic Caspase 
ProteomicsMPP profiling in yeast - Quantitative profiling for substrates of the mitochondrial presequence processing protease reveals a set of non-substrate proteins increased upon proteotoxic stress
The majority of mitochondrial preproteins is targeted via N-terminal presequences that are cleaved upon import into the organelle. The essential mitochondrial processing protease (MPP) is assumed to cleave the majority of incoming precursors. However, only few substrate proteins of MPP have been exp...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2015-10-14 | PXD002063 | Pride
Mitochondrial processing protease mpp Mitochondria Chafradic Mitochondrial stress response Presequence import pathway 
ProteomicsChaFRAtip - Simple, scalable and ultra-sensitive tip-based identification of protease substrates
Enrichment of N-terminal peptides using HPLC-based ChaFRADIC and the novel tip-based ChaFRAtip method.
ORGANISM(S): Homo sapiens (Human) 
2018-01-24 | PXD006595 | Pride
N-terminomics 
ProteomicsThe mitochondrial CLPXP complex supports the coordination and assembly of mitochondrial and nuclear encoded protein complexes in Arabidopsis thaliana
The mitochondrial CLPXP complex supports the coordination and assembly of mitochondrial and nuclear encoded protein complexes in Arabidopsis thaliana. Protein labeling using iTRAQ reagents and application of optimized ChaFRADIC technology to enrich N-Terminal peptides.
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2020-09-14 | PXD016263 | Pride
Genome coordination Rnaseq Proteomics Chafradic Combined omics Clpxp 
ProteomicsMild proteasomal stress improves photosynthetic performance in Arabidopsis chloroplasts
The ppi2 mutant introgressed into the Columbia-0 ecotype as previously reported. Doublemutant ppi2xrpn8a was crossed with the above referenced genotypes using heterozygous ppi2 plants. After 2 days of stratification at 4 °C the plants were grown on half-strength Murashige and Skoog (M&S) medium supp...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2020-05-27 | PXD017126 | Pride
Arabidopsis Chafradic N-termiomics 
ProteomicsCombined assessment of global proteome, phosphoproteome and N-terminal protein cleavage to characterize altered platelet functions in human Scott syndrome
The Scott syndrome is a rare bleeding disorder associated with a mutation in the gene encoding anoctamin-6 (TMEM16F). After stimulation of Ca2+-mobilizing agonists, syndromatic platelets show a reduced phosphatidylserine exposure and do not form membrane blebs. Given the central role of anoctamin-6 ...
ORGANISM(S): Homo sapiens (Human) 
2016-08-24 | PXD002883 | Pride
Phosphoproteome Human platelets N-terminal chafradic Lc-ms Proteome 
ProteomicsSimple, scalable and sensitive tip-based identification of protease substrates
Large-scale N-terminomics using COFRADIC and TAILS has revolutionized protease research, but is disseminated only in few labs. We present an alternative and simple protocol for N-terminal peptide enrichment, based on charge-based fractional diagonal chromatography (ChaFRADIC) and requiring only well...
ORGANISM(S): Homo sapiens (Human) 
2018-01-24 | PXD005954 | Pride
Human Lc-ms Scx 
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