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MetabolomicsDissection of two routes to naïve pluripotency using different kinase inhibitors
Embryonic stem cells (ESCs) can be maintained in the naïve state through inhibition of Mek1/2 and Gsk3 (2i). A relevant effect of 2i is the inhibition of Cdk8/19, which are negative regulators of the Mediator complex, responsible for the activity of enhancers. Inhibition of Cdk8/19 (Cdk8/19i) stimul...
2021-02-10 | MTBLS301 | MetaboLights
ProteomicsProteomics of Secretomes and Exosomes in Melanoma
Exosomes were purified from several cell lines with different metastatic potential. Seecreted proteins were isolated from the same samples using the approach from Eichelbaum et al. Nat Biotechnol 2012
ORGANISM(S): Homo sapiens (Human) 
2017-06-29 | PXD002703 | Pride
Melanoma Proteomics Secretomes Metastasis Exosomes 
UnknownUltrasonography effectiveness of the vibration vs cryotherapy added to an eccentric exercise protocol in patients with chronic mid-portion Achilles tendinopathy: A randomised clinical trial.
Not available
S-EPMC7948929 | biostudies-literature
ProteomicsMASS SPECTROMETRY ROADMAPS TO NAIVE PLURIPOTENCY
Pluripotency can be maintained in the naïve state through manipulation of ERK and WNT signalling (2i), shielding embryonic stem cells (ESCs) from inductive cues. Alternatively, inhibiting CDK8/19 (CDK8/19i), a repressor of the Mediator co-activator complex, directly stimulates super-enhancer activit...
ORGANISM(S): Mus musculus (Mouse) 
2021-02-10 | PXD018694 | Pride
Phosphoproteomics Mouse stem cells Proteomics 
ProteomicsThe RNA Polymerase II factor RPAP1 is critical for Mediator-driven transcription and cell identity
The RNA Polymerase II Associated Protein 1 (RPAP1) is conserved across metazoa and critical for stem cell differentiation in plants, however, very little is known about its mechanism of action or its role in mammalian cells. Here, we report that RPAP1 is essential for the expression of lineage spec...
ORGANISM(S): Mus musculus (Mouse) 
2018-01-10 | PXD007114 | Pride
Cell identity Rna pol ii Rpap1 Mediator 
ProteomicsHuman, melanoma, p62/SQSTM1, quantitative proteomics
p62/SQSTM1 was identified as a modulator of metastatic genes selectively enriched in melanoma in autophagy independent manner. iTRAQ quantitative proteomic approach was performed in melanoma cell lines (SK-Mel-103 and UACC-62) deficient for p62 to identify downstream effectors of p62. Similar studie...
ORGANISM(S): Homo sapiens (Human) 
2022-02-28 | PXD006650 | Pride
Human Ap-ms P62/sqstm1 Melanoma cell lines Quantitative proteomics Lc-ms/ms 
ProteomicsQuantitative proteomics of CELF1-silenced human melanoma cell lines
CELF1 was silenced in two human melanoma cell lines (SKMEL-103 and UACC-62, indicated as 9M and 17M, respectively) using short hairpin RNA (tag 114). As control scrambled non targeting shControl transduced cell were used (tag 115).
ORGANISM(S): Homo sapiens (Human) 
2017-12-21 | PXD003112 | Pride
Human Lc-ms/ms Velos orbitrap Melanoma Itraq Celf1 
UnknownCARB-ES-19 Multicenter Study of Carbapenemase-Producing Klebsiella pneumoniae and Escherichia coli From All Spanish Provinces Reveals Interregional Spread of High-Risk Clones Such as ST307/OXA-48 and ST512/KPC-3.
Not available
S-EPMC9279682 | biostudies-literature
UnknownEffect of Direct Transportation to Thrombectomy-Capable Center vs Local Stroke Center on Neurological Outcomes in Patients With Suspected Large-Vessel Occlusion Stroke in Nonurban Areas: The RACECAT Randomized Clinical Trial.
Not available
S-EPMC9073661 | biostudies-literature
UnknownImpact of neuromuscular block monitoring and reversal on postoperative pulmonary complications in thoracic surgery: a Bayesian analysis of the iPROVE-OLV trial.
Not available
S-EPMC12597387 | biostudies-literature
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