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MetabolomicsCE-MS Metabolomic and LC-MS Proteomic Analyses of Breast Cancer Exosomes Reveal Alterations in Purine and Carnitine Metabolism

A nano sheath-flow capillary electrophoresis mass spectrometry (CE-MS) system with electrospray ionization was used to profile cationic metabolite cargo in exosomes secreted by non-tumorigenic MCF-10A and tumorigenic MDA-MB-231 breast epithelial cells. CE-MS-based metabolomics revealed increased ...

2024-11-14 | MTBLS11190 | MetaboLights
ProteomicsBenchmarking low- and high-throughput protein cleanup and digestion methods for human fecal metaproteomics
Benchmarking low- and high-throughput protein cleanup and digestion methods for human fecal metaproteomics
ORGANISM(S): Homo sapiens (Human) human gut metagenome 
2024-05-31 | PXD049294 | Pride
Metaproteome Mass spectrometry Fasp Gut microbiota Sample preparation Multiwell plate 
ProteomicsLaser capture microdissection-capillary zone electrophoresis-tandem mass spectrometry (LCM-CZE-MS/MS) for spatially resolved top-down proteomics: a pilot study of zebrafish brain
Mass spectrometry (MS)-based spatially resolved top-down proteomics (TDP) of tissues is crucial for understanding the roles played by microenvironmental heterogeneity in the biological functions of organs and for discovering new proteoform biomarkers of diseases. There are few published spatially re...
ORGANISM(S): zebrafish metagenome 
2023-03-11 | PXD027741 | Pride
Spatially resolved top-down proteomics  capillary zone electrophoresis-tandem mass spectrometry  laser capture microdissection  zebrafish brain  proteoform  high sensitivity  neuropeptide hormone activity  motor activity 
UnknownPilot Evaluation of the Long-Term Reproducibility of Capillary Zone Electrophoresis-Tandem Mass Spectrometry for Top-Down Proteomics of a Complex Proteome Sample.
Not available
S-EPMC11002928 | biostudies-literature
ProteomicsPilot evaluation of long-term reproducibility of capillary zone electrophoresis-tandem mass spectrometry for top-down proteomics of a complex proteome sample
In this work, for the first time, we performed a pilot investigation of long-term reproducibility of CZE-MS for TDP of a complex sample (i.e., a yeast cell lysate) to achieve a better understanding of advantages, issues and potential solutions of CZE-MS for large-scale TDP.
ORGANISM(S): Candida albicans (Yeast) Saccharomyces cerevisiae 
2024-03-20 | PXD046651 | Pride
Yeast proteome Capillary zone electrophoresis-tandem ms (cze-ms/ms) Top-down proteomics (tdp) Capillary cleanup Long-term reproducibility 
UnknownCombining SDS-PAGE to capillary zone electrophoresis-tandem mass spectrometry for high-resolution top-down proteomics analysis of intact histone proteoforms.
Not available
S-EPMC11647866 | biostudies-literature
UnknownLaser capture microdissection-capillary zone electrophoresis-tandem mass spectrometry (LCM-CZE-MS/MS) for spatially resolved top-down proteomics: a pilot study of zebrafish brain.
Not available
S-EPMC9066772 | biostudies-literature
ProteomicsTowards a universal sample preparation method for denaturing top-down proteomics of complex proteomes
A universal and standardized sample preparation method becomes vital for denaturing top-down proteomics (dTDP) to advance the scale and accuracy of proteoform delineation in complex biological systems. It needs to have high protein recovery, minimum bias, good reproducibility, and compatibility with...
ORGANISM(S): Homo sapiens (Human) Escherichia coli 
2020-05-22 | PXD018248 | Pride
Histone Ptms Membrane ultrafiltration Chloroform-methanol precipitation Membrane proteins Sp3 Denaturing top-down proteomics Cze-ms/ms Sds Sample preparation 
TranscriptomicsDNA methylation regulates lineage-specifying genes in the human vascular system [methylation array].
During embryonic development, the lymphatic system emerges by transdifferentiation from the cardinal vein. Although lymphatic and blood vasculature share a close molecular and developmental relationship, they display distinct features and functions. However, even after terminal differentiation, tran...
ORGANISM(S): Homo sapiens 
2012-04-09 | E-GEOD-34486 | biostudies-arrayexpress
TranscriptomicsAcute Lung Injury In Experimental Malaria
Although respiratory distress is a common complication of severe malaria, little is known about the underlying molecular basis of lung dysfunction. Animal models have provided powerful insights into the pathogenesis of severe malaria syndromes such as cerebral malaria; however, no model of malaria-i...
ORGANISM(S): Mus musculus 
2010-05-26 | E-GEOD-9497 | biostudies-arrayexpress
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