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ProteomicsChemoproteomics of an NSD2-PWWP1 Chemical Probe
Here we use competitive chemoproteomics pulldowns followed by label-free quantitative LC-MS/MS to assess target engagement and selectivity profiles of UNC6934 and UNC7145, a chemical probe targeting the PWWP1 domain of NSD2 and its negative control counterpart, respectively. To this end, we used a b...
ORGANISM(S): Homo sapiens (Human) 
2021-08-06 | PXD017641 | Pride
Epigenetics Chemoproteomics Histone methyltransferase Chemical probe 
ProteomicsActivity-based profiling of Shigella virulence factor OspF in human cells
Phosphothreonine lyases are a small family of bacterial virulence factors secreted into host cells during infection by certain Gram-negative pathogens. This family of enzymes is known for its unique mechanism of silencing host immune signaling via the irreversible β-elimination of phosphothreonine (...
ORGANISM(S): Homo sapiens (Human) 
2026-02-27 | PXD073985 | Pride
Ospf Dehydrobutyrine Chemoproteomics Phosphothreonine lyase Shigella Bacterial infection Human 
UnknownA Highly Reactive Cysteine-Targeted Acrylophenone Chemical Probe That Enables Peptide/Protein Bioconjugation and Chemoproteomics Analysis.
Not available
S-EPMC12728623 | biostudies-literature
GenomicsComprehensive landscape of active deubiquitinating enzymes profiled by advanced chemoproteomics
Comprehensive landscape of active deubiquitinating enzymes profiled by advanced chemoproteomics
PRJNA556874 | ENA
ProteomicsChemoproteomics with a Diaryliodonium Probe in Bacteria
The alarming increase in antimicrobial resistance (AMR) will soon limit treatment options for even routine infections, especially those caused by Gram-negative bacteria such as Acinetobacter baumannii. Here, we report a two-pronged approach to identify new vulnerabilities in such pathogens. Staring ...
ORGANISM(S): Escherichia coli Acinetobacter baumannii (strain ATCC 17978 / CIP 53.77 / LMG 1025 / NCDC KC755 / 5377) 
2023-03-29 | PXD033116 | Pride
Lc-ms/ms Chemoproteomics 
ProteomicsMultiplexed CuAAC-Suzuki-Miyaura for tandem activity-based chemoproteomic profiling
Suzuki-Miyauara cross-coupling has been used for mass-spectrometry-based chemoproteomics, including for target deconvolution and site of labeling studies. We also demonstrated that Suzuki-Miyauara cross coupling and CuAAC can be combined for dual target labeling for mass spectrometry-based proteomic...
ORGANISM(S): Homo sapiens (Human) 
2021-02-03 | PXD022279 | Pride
Multiplexed cuaac suzuki-miyaura chemoproteomics Sp3 cleanup Suzuki-miyauara cross-coupling 
ProteomicsBenchmarking cleavable biotin tags for site-centric chemoproteomics
the performance of five common cleavable biotin linkers are assessed parallelly by using commercially available thiol-reactive probe IPM (2-iodo-N-(prop-2-yn-1-yl)acetamide) in profiling cysteinome
ORGANISM(S): Homo Sapiens 
2022-01-16 | PXD031019 |
ProteomicsRobust proteome profiling of cysteine-reactive fragments using label-free chemoproteomics - 80 compound screen (HEK293T and Jurkat)
In this manuscript we describe our work on the development of a label-free chemoproteomics screening platform for cysteine reactive covalent fragments on a 96 well plate format. Within this workflow, we profile cysteine reactive fragments by competition with the hyper-reactive iodoacetamide desthiob...
ORGANISM(S): Homo sapiens (Human) 
2024-11-18 | PXD054127 | Pride
Chemoproteomics Data independant acquisition Label-free qauntification 
TranscriptomicsComprehensive landscape of active deubiquitinating enzymes profiled by advanced chemoproteomics
Enzymes that bind and process ubiquitin, a small 76 amino acid protein, have been recognized as pharmacological targets in oncology, immunological disorders and neurodegeneration. Mass spectrometry technology has now reached the capacity to cover the proteome with enough depth to interrogate entire ...
ORGANISM(S): Homo sapiens 
2019-08-31 | GSE134954 | GEO
ProteomicsValidation of PG-DA probe in mammalian cell and brain lysates
Here we aim to characterize the 1-palmitoyl-glycerol diazirine alkyne (PGDA) probe using chemical proteomics approaches in mammalian cell and mouse brain lysates.
ORGANISM(S): Mus musculus (Mouse) 
2025-06-24 | PXD051332 | Pride
Chemoproteomics Lipid probe Mag 
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