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ProteomicsIdentification of novel KRT32-associated proteins in primary keratinocytes cells lysate by GST-KRT32 pulldown assays coupled with mass spectrometry.
GST-KRT32 and its interacting proteins in primary keratinocytes cells were enriched by pull-down using glutathione-Sepharose beads. Then, KRT32-interacting proteins were identified by mass spectrum. Proteins obtained in Pulldown were subjected to SDS-PAGE gel electrophoresis and silver staining. The...
ORGANISM(S): Homo sapiens (Human) 
2024-06-17 | PXD050141 | Pride
Keratin  krt32 
ProteomicsEMP1 preserves hematopoietic stem cell potential under homeostasis and radiation-induced injury by alleviating ER stress
To illustrate EMP1-interacting proteins, we performed co-immunoprecipitation (co-IP) followed by mass spectrometry using HSPCs (Lin-c-Kit+) to characterize protein interactomes of EMP1. Murine HSPCs (Lin-c-Kit+) were infected with retrovirus to force EMP1 overexpression (with HA tag). Then IgG or HA...
ORGANISM(S): Mus musculus (Mouse) 
2025-03-13 | PXD055884 | Pride
Hspcs Emp1 
ProteomicsHK-2 cells 4D label quantitative lactylproteomic analysis
Following acute kidney injury (AKI), a substantial subset of patients experiences an irreversible progression to chronic kidney disease (CKD), yet the molecular determinants governing this maladaptive transition remain elusive, and effective clinical interventions are lacking. Here, we identify lact...
ORGANISM(S): Homo sapiens (Human) 
2026-07-18 | PXD078736 | Pride
Human  lactylation 
ProteomicsSerum proteomics of wild type mice and Mdm2 adipocyte-specific knock-in mice fed high-fat diet
The serum samples from wild type mice fed high-fat diet for 12 weeks (WT_Serum) and Mdm2 adipocyte-specific knock-in mice fed high-fat diet for 12 weeks (KI_Serum) were mixed separately, and subjected to proteomic study by Label-free quantitative techniques and mass spectrometry-based proteomics tec...
ORGANISM(S): Mus musculus (Mouse) 
2022-08-11 | PXD034069 | Pride
Serum Proteomics Mice Wild type Mdm2 
ProteomicsThe eWAT proteomics of wild type mice and Mdm2 adipocyte-specific knock-in mice fed high-fat diet
The epididymal adipose tissue (eWAT) samples from wild type mice fed high-fat diet for 12 weeks (H_WT_E) and Mdm2 adipocyte-specific knock-in mice fed high-fat diet for 12 weeks (H_KI_E) were mixed separately, and subjected to proteomic study by Label-free quantitative techniques and mass spectromet...
ORGANISM(S): Mus musculus (Mouse) 
2022-08-11 | PXD034074 | Pride
Proteomics Ewat Mice Mdm2 High-fat diet 
ProteomicsProteomic analysis of Masson pine with high resistance to pine wood nematode
Pine wilt disease is a worldwide dangerous pine disease. We used Masson pine (Pinus massoniana) clones, selected through traditional breeding and testing for 20 years, with high resistance to study the molecular mechanism of resistance to pine wood nematode (PWN, Bursaphelenchus xylophilus). A total...
ORGANISM(S): Pinus massoniana var. massoniana 
2022-10-13 | PXD030664 | Pride
Masson pine  pine wood nematode  resistant proteins  proteomic analysis 
Proteomicsβ-catenin-controlled tubular cell-derived exosomes play a key role in fibroblast activation via the OPN-CD44 axis
Tubular injury and peripheral fibroblast activation are the hallmarks of chronic kidney disease (CKD) and renal fibrosis, suggesting intimate communication between the two diseases. However, the underlying mechanisms remain to be determined. Exosomes play a role in shuttling proteins and other mater...
ORGANISM(S): Homo sapiens (Human) 
2022-05-20 | PXD030696 | Pride
Opn Cd44 Exosomes Β-catenin Renal fibrosis 
UnknownQuantitative global proteome and lysine succinylome analyses provide insights into metabolic regulation and lymph node metastasis in gastric cancer.
Not available
S-EPMC5292683 | biostudies-literature
ProteomicsRPL6 interacts with HMGCS1 to stabilize HIF-1α by promoting cholesterol production in hepatocellular carcinoma.
The identification of predictive markers to determine the premetastatic phase before metastasis is critical for developing effective strategies for early detection and prevention. By applying the dynamic network biomarker (DNB) approach to analyze time-series transcriptomic data from a pulmonary met...
ORGANISM(S): Homo sapiens (Human) 
2025-10-20 | PXD064850 | Pride
Hepatocellular carcinoma  metastasis  rpl6  hmgcs1  cholesterol 
ProteomicsRPL6 interacts with HMGCS1 to stabilize HIF-1α by activating cholesterol biosynthesis in hepatocellular carcinoma.
The identification of predictive markers to determine the premetastatic phase before metastasis is critical for developing effective strategies for early detection and prevention. By applying the dynamic network biomarker (DNB) approach to analyze time-series transcriptomic data from a pulmonary met...
ORGANISM(S): Homo sapiens (Human) 
2025-10-20 | PXD054740 | Pride
Hepatocellular carcinoma  metastasis  rpl6  hmgcs1  cholesterol 
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