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TranscriptomicsTranscription profiling of human diploid and tetraploid HCT116 cells treated with cisplatin and/or a Chk1 inhibitor to investigate resistance to genotoxic stress
Tetraploid cells are more resistant against genotoxic stress (irradiation, platinum compounds, topoisomerase inhibitors) than their diploid precursors. Here, we studied the effect of cisplatin and/or Chk1 inhibition on the transcriptome of diploid and tetraploid HCT116 cells
ORGANISM(S): Homo sapiens 
2008-06-09 | E-TABM-172 | biostudies-arrayexpress
Proteomicsβ-tubulin phosphorylation by Chk1 is required for normal spindle formation during cell division
The mitotic spindle is a microtubule-based apparatus that is responsible for accurate segregation of chromosomes into two daughter cells. In this study, we show that the DNA damage kinase Chk1 is required for optimal density and efficient polymerization of spindle microtubules during unperturbed mit...
ORGANISM(S): Homo sapiens (Human) Spodoptera frugiperda 
2026-06-08 | PXD074543 | Pride
Atr Atrip Topbp1 Centrosome Chromosome segregation -tubulin Chk1 Cell proliferation Mitotic spindle 
TranscriptomicsTRRAP-mediated regulation of Wee1
The G2 DNA damage checkpoint inhibits Cdc2 and mitotic entry through the dual regulation of Wee1 and Cdc25 by the Chk1 effector kinase. Up-regulation of Chk1 by mutation or overexpression bypasses the requirement for up-stream regulators or DNA damage to promote a G2 cell cycle arrest. We screened i...
ORGANISM(S): Schizosaccharomyces pombe 
2010-04-11 | E-GEOD-12674 | biostudies-arrayexpress
ProteomicsPhosphoproteomics analysis of CHK1 inhibitor treatment
Inhibitors of checkpoint kinase 1 (CHK1),a central component of DNA damage and cell cycle checkpoint response, represent a promising new cancer therapy, but the global cellular functionsthey regulate through phosphorylationare poorly understood. To elucidate the CHK1-regulated phosphorylation networ...
ORGANISM(S): Homo sapiens (Human) 
2020-04-23 | PXD015125 | Pride
Chk1 inhibitor Silac Phosphoprotomics 
ProteomicsSPARTAN Protease and Checkpoint Kinase 1 Cross-Activation Loop Safeguards Genome Stability
DNA-protein crosslinks (DPCs) are abundant DNA lesions, which constantly challenge genome stability by interfering with DNA replication. SPARTAN (SPRTN) protease plays a central role in DPCs repair in proliferative vertebrate cells. SPRTN is a constitutive part of the DNA replication machinery and i...
ORGANISM(S): Homo sapiens (Human) 
2019-11-08 | PXD006741 | Pride
Cancer Spartan protease Dna-protein crosslinks Ageing Genome stability Chk1 kinase Dna replication 
ProteomicsThe phosphoproteomics study of Chk1 and ATR inhibitors regulation on cells
Ataxia Telangiectasia and Rad3-related (ATR) and Checkpoint Kinase 1 (Chk1) are crucial kinases in the DNA Damage Response (DDR) pathway. While the roles of ATR and Chk1 within the DDR are well-established, their roles in mitosis are not fully understood. Here, we describe that the ATR-Chk1 pathway ...
ORGANISM(S): Homo sapiens (Human) 
2025-06-23 | PXD058541 | Pride
Phosphoproteomics Cdk1 Inhibitor Chk1 
ProteomicsIdentification of post translational modifications of human Topoiosmerase 1 in the presence of camptothecin and CHK1 inhibitor SCH900776.
Topisomerase 1 (TOP1), an essential enzyme in humans, plays a critical role in relaxation of DNA supercoils during replication and transcription. The TOP1 reaction cycle involves an obligate TOP1-DNA covalent complex intermediate (TOP1cc), which is stabilized by TOP1 poisons like camptothecin. While...
ORGANISM(S): Homo sapiens (Human) 
2026-03-16 | PXD055870 | Pride
Topoisomerase 1 Mk-8776 Chk1 Sch900776 Camptothecin 
TranscriptomicsExpression data of CHK1 shRNA knockdown and control shRNA in U2OS cells
Homologous recombination-mediated DNA repair deficiency (HRD) predisposes to cancer development, but also provides therapeutic opportunities. Here, we identified an HRD gene signature that robustly predicted HRD status. Unexpectedly, concurrent loss of PTEN in BRCA1-deficient cells might extensively...
ORGANISM(S): Homo sapiens 
2014-01-24 | E-GEOD-54267 | biostudies-arrayexpress
TranscriptomicsCastalin induces ROS production, leading to DNA damage and increasing the activity of CHK1 inhibitor in cancer cell lines
Background: cancer therapy is one of the challenging arguments in constant development. One of the principal problems connected with the tumor therapy arise form the potential side effects connected with the classical chemotherapeutic treatment but also with the molecular target therapy. The identif...
ORGANISM(S): Homo sapiens 
2025-09-04 | E-MTAB-15334 | biostudies-arrayexpress
UnknownKinase-independent function of checkpoint kinase 1 (Chk1) in the replication of damaged DNA.
Not available
S-EPMC3358916 | biostudies-literature
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