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TranscriptomicsRNA-seq analysis determined the effect of pat or cobB mutation on transcriptional levels in Salmonella Typhimurium 14028s.
Salmonella causes a range of diseases in different hosts, including enterocolitis and systemic infection. Lysine acetylation regulates many eukaryotic cellular processes, but its function in prokaryotes is largely unknown. Reversible acetylation in Salmonella Typhimurium depends on acetyltransferase...
ORGANISM(S): Salmonella enterica subsp. enterica serovar Typhimurium str. LT2 
2016-08-10 | E-GEOD-71907 | biostudies-arrayexpress
TranscriptomicsEscherichia coli BW25113 cobB/pCA24N-cobB vs. cobB/pCA24N under stress conditions
Acetylation of lysine residues is conserved in all three kingdoms; however, its role in prokaryotes is unknown. Here we demonstrate that acetylation enables the reference bacterium Escherichia coli to withstand environmental stress. Specifically, the bacterium reaches higher cell densities and beco...
ORGANISM(S): Escherichia coli 
2012-06-05 | E-GEOD-29803 | biostudies-arrayexpress
GenomicsCOBB
Ceca-COBB
PRJEB78857 | ENA
Proteomicsc-di-GMP affect the protein acetylation through CobB dependent deacetylation
To determine whether c-di-GMP could affect CobB-dependent deacetylation in a global setting, we applied Stable Isotope Labeling with Amino acids in Cell culture (SILAC) coupled with MS to quantitatively compare the levels of protein acetylation in WT, ΔcobB and ΔdgcZ cells.
ORGANISM(S): Escherichia coli 
2019-06-17 | PXD013292 | Pride
C-di-gmp cobb 
ProteomicsSILAC labeling efficiency for c-di-GMP and CobB
Description To determine whether c-di-GMP could affect CobB-dependent deacetylation in a global setting, we applied Stable Isotope Labeling with Amino acids in Cell culture (SILAC) coupled with MS to quantitatively compare the levels of protein acetylation in WT, ΔcobB and ΔdgcZ cells. Before that, ...
ORGANISM(S): Escherichia coli 
2019-06-24 | PXD014344 | Pride
Silac c-di-gmp 
TranscriptomicsEscherichia coli BW25113 cobB/pCA24N-cobB vs. cobB/pCA24N under stress conditions
Acetylation of lysine residues is conserved in all three kingdoms; however, its role in prokaryotes is unknown. Here we demonstrate that acetylation enables the reference bacterium Escherichia coli to withstand environmental stress. Specifically, the bacterium reaches higher cell densities and becom...
ORGANISM(S): Escherichia coli 
2012-06-06 | GSE29803 | GEO
ProteomicsAccurate quantification of site-specific acetylation stoichiometry reveals the impact of sirtuin deacetylase CobB on the E. coli acetylome
We applied SD-SILAC to determine absolute acetylation stoichiometry in exponentially-growing and stationary-phase wild type and sirtuin deacetylase CobB-deficient E. coli. We further assayed stoichiometry in phophotransacetylase (pta) and acetate kinase (ackA) mutant strains in the presence and abse...
ORGANISM(S): Escherichia coli 
2017-03-07 | PXD005757 | Pride
E. coli Sirtuin Acetylation Stoichiometry Cobb 
ProteomicsSILAC experiments profiling of the proteome of WT and CobB KO E.coli
SILAC experiments profiling of the proteome of WT and CobB KO E.coli
ORGANISM(S): Escherichia Coli 
2021-12-11 | PXD030345 |
ProteomicsProteomics analysis reveals the effect of Aeromonas hydrophila sirtuin CobB on biological functions
a DIA-based proteomics method was used to compare the differently protein abundance between cobB deleted mutant and wild type strain.
ORGANISM(S): Aeromonas Hydrophila 07-345-3437 
2020-12-31 | PXD023330 |
ProteomicsProtein Deacetylase CobB Interplays with c-di-GMP
As a ubiquitous bacterial secondary messenger, c-di-GMP plays key regulatory roles in processes like bacterial motility and transcription regulation. We found that c-di-GMP had impact on lysine acetylation level. To further investigate the regulation role, we performed a quantitative analysis of E. ...
ORGANISM(S): Escherichia coli 
2019-06-17 | PXD010558 | Pride
C-di-gmp Ydeh Acetylation 
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