Sort   by:  
 Page size 
ProteomicsTandem LysC/trypsin digestion performs efficient in detergent conditions and under constant denaturating environments
All shotgun proteomics experiments rely on efficient proteolysis steps for sensitive peptide/protein identification and quantification. Continuous protocol improvement is therefore a constant topic in proteomics research. Previous reports suggest that the sequential tandem LysC/trypsin digest yields...
ORGANISM(S): Escherichia coli Bacteria 
2020-04-30 | PXD013273 | Pride
Shotgun proteomics Protein digestion Lysc Trypsin 
ProteomicsProfiling of Subclass-Specific IgG Glycosylation in Colorectal Cancer
Changes in IgG glycosylation have been reported to be associated with colorectal cancer (CRC), but the alteration in specific subclass of IgG is unknown. Herein, we optimized five common IgG glycopeptides enrichment methods to acquire comprehensive profiling of IgG glycoproteomics in CRC. Notably, i...
ORGANISM(S): Homo sapiens (Human) 
2022-10-27 | PXD016318 | Pride
Human Serum igg Complete tryptic digestion Colorectal Lc-ms/ms 
ProteomicsHit-Gel: Streamlining in-gel protein digestion for high-throughput proteomics experiments.
Traditional in gel-digestion procedures require extensive sample handling, are prone to contamination and not compatible with high-throughput sample preparation. To address these shortcomings, we have modified the conventional in-gel digestion procedure for high-throughput proteomics studies. The mo...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2018-10-16 | PXD009510 | Pride
Large-scale In-gel digestion Proteomics Tryptic digest Fractionation Native protein complex Sample preparation High-throughput Lc-ms/ms 
ProteomicsSequential digestion with Trypsin and Elastase in cross-linking/mass spectrometry
Cross-linking/mass spectrometry has become an important approach for studying protein structures and protein-protein interactions. The amino acid composition of some protein regions impedes the detection of cross-linked residues, although it would yield invaluable information for protein modelling. ...
ORGANISM(S): Homo sapiens (Human) Schizosaccharomyces pombe 
2019-03-07 | PXD011459 | Pride
Lc-msms Cross-linking Yeast Human taf4-12 complex 
ProteomicsMulti-Enzymatic Limited Digestion - the Next-Generation Sequencing for Proteomics
An innovative approach merging both the bottom-up and middle-down ideologies to provide fast, robust and comprehensive solutions for proteomics studies. The methodology, referred to as MELD for Multiple-Enzymatic Limited Digestion, capitalizes on a single two-hour long proteolytic step involving the...
ORGANISM(S): Homo sapiens (Human) 
2019-05-08 | PXD009800 | Pride
Ptms Digestibility profile Limited digestion Mass spectrometry Proteolytic method Protein characterization De novo sequencing  mabs 
UnknownThe NISTmAb tryptic peptide spectral library for monoclonal antibody characterization.
Not available
S-EPMC5916550 | biostudies-literature
ProteomicsRefinement of paramagnetic bead–based digestion protocol for automatic sample preparation using an artificial neural network
Despite technological advances in the proteomics field, sample preparation still represents the main bottleneck in mass spectrometry (MS) analysis. Bead-based protein aggregation techniques have recently emerged as an efficient, reproducible, and high-throughput alternative for protein extraction an...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) Mus musculus (Mouse) Homo sapiens (Human) Escherichia coli Rattus norvegicus (Rat) 
2024-05-31 | PXD048358 | Pride
Bile Magnetic beads Protein aggregation Sp3 protein digestion Artificial neural network 
ProteomicsGastrointestinal digestion of hazelnut allergens on molecular level: Elucidation of degradation kinetics and resistant immunoactive peptides using mass spectrometry
Allergy to hazelnut seeds ranks among the most prevalent food allergies in Europe. The aim of this study was to elucidate the gastrointestinal digestion of hazelnut allergens on molecular level. Hazelnut flour was digested in vitro following the Infogest consensus model. For six allergenic proteins,...
ORGANISM(S): Corylus avellana 
2017-07-11 | PXD006412 | Pride
Food allergy Hazelnut Digestion Allergen 
ProteomicsProteomeDiscover Crosslinking Complete Submission - HEK 293 XL fractions analysis
The following dataset is an example and gold-standard dataset of a Crosslinking (XL) Complete submission to PRIDE Archive using mzIdentML and the guidelines for XL submissions. The dataset is a HEK 293 sample analyzed with multiple fractions.
ORGANISM(S): Homo sapiens (Human) 
2025-01-16 | PXD059766 | Pride
Benchmark Crosslinking 
ProteomicsUltraviolet Photodissociation of tryptic peptide backbones at 213 nm
We analyzed the backbone fragmentation behavior of tryptic peptides of a four protein mixture and of E. coli lysate subjected to Ultraviolet Photodissociation (UVPD) at 213 nm on a commercially available UVPD-equipped tribrid mass spectrometer. We obtained 15,178 high-confidence peptide-spectrum mat...
ORGANISM(S): Homo sapiens (Human) Oryctolagus cuniculus (Rabbit) Escherichia coli Gallus gallus (Chicken) Equus caballus (Horse) 
2020-05-07 | PXD018176 | Pride
Lc-msms E. coli Ovotransferrin Fusion lumos Dual ms2 Uvpd 213 nm Hsa Myoglobin Creatine kinase 
Sort   by:  
 Page size