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ProteomicsQuantitative profiling of STOichiometry by Resolvable Mass tags
Post-translational modifications (PTMs) play important roles in modulating biological functions of proteins. Stoichiometry, which quantifies the modification percentage, is a critical factor for any given PTM. In this work, we developed a chemoproteomic strategy called “STO-MS” to systematically qua...
ORGANISM(S): Homo Sapiens 
PXD056632 |
TranscriptomicsGenome-wide maps of primary and processed start-sites of transcripts revealed mechanism controlling in vivo stoichiometry of protein complex in bacteria
Here a differential mRNA-sequencing (dRNA-Seq) strategy was employed to distinguish transcriptional start-sites (TSs) and post-transcriptional processed sites (PSs) of transcripts in the cellulolytic mesophile Clostridium cellulolyticum. PS-harboring genes usually  encode heteromultimeric complexes...
ORGANISM(S): [Clostridium] cellulolyticum 
2015-04-27 | E-GEOD-57652 | biostudies-arrayexpress
ProteomicsStoichiometry of the bacterial type III secretion export apparatus
Bacterial type III secretion systems are cell envelope-spanning effector protein-delivery machines essential for colonization and survival of many Gram-negative pathogens and symbionts. The membrane-embedded core unit of these secretion systems is termed needle complex. The needle complex is compose...
ORGANISM(S): Salmonella enterica subsp. enterica serovar Typhimurium str. SL1344 
2016-02-25 | PXD003113 | Pride
Mass spectrometry Stoichiometry Salmonella Type iii secretion 
ProteomicsComparison of the composition and interaction partners of the Nucleosome Remodeling and Deacetylase (NuRD) complex in cancer cells
The Nucleosome Remodeling and Deacetylase (NuRD) complex is essential for normal development in metazoans and has direct implications in a range of human diseases. Amongst all classes of chromatin remodeling complexes, the NuRD complex has been the most recalcitrant to analysis. This is, in part, du...
ORGANISM(S): Homo sapiens (Human) 
2019-03-13 | PXD012061 | Pride
Shotgun proteomics Ibaq Stoichiometry Nurd Interactome 
ProteomicsStoichiometry of two plant glycine decarboxylase complexes and comparison with a cyanobacterial glycine cleavage system
The multienzyme glycine cleavage system (GCS) converts glycine and tetrahydrofolate to the one-carbon compound 5,10-methylenetetrahydrofolate, which is of vital importance for most if not all organisms. Photorespiring plant mitochondria contain very high levels of GCS proteins organized as a fragile...
ORGANISM(S): Pisum sativum (Garden pea) Arabidopsis thaliana (Mouse-ear cress) 
2020-04-23 | PXD018211 | Pride
Arabidopsis Pea Quantification Stoichiometry Qconcat Glycine decarboxylase complex 
ProteomicsStoichiometry of MBD2 vs. MBD3-NuRD
To obtain a comprehensive and quantitative view on MBD2 vs MBD3-NuRD complex stoichiometry, we performed biotin co-immunoprecipitations in Mbd3 KO ES cells expressing either biotin-tagged MBD2a or MBD3a and identified known NuRD complex members using label-free mass spectrometry (Supplementary Fig. ...
ORGANISM(S): Mus musculus (Mouse) 
2023-06-30 | PXD042407 | Pride
Embryonic stem cells Nurd complex 
ProteomicsIntegrated lysine acetylation stoichiometry and proteomics analysis in human cells
Lysine acetylation is a widespread posttranslational modification that targets a large number of biological pathways. Recent studies reveal that lysine acetylation sites exhibit mainly low stoichiometry. Here we explored three sample preparation methods, the use of detergents for the chemical acetyl...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2017-11-09 | MSV000081704 | MassIVE
UnknownStoichiometry and structure of a lantibiotic maturation complex.
Not available
S-EPMC5294574 | biostudies-literature
ProteomicsMass Spectrometry Uncovers Protein Interactions and Stoichiometry of Intermediates and Sub-complexes for SNARE Complex Assembly
The neuronal SNARE complex assembles from vesicular Synaptobrevin-2 as well as Syntaxin-1 and SNAP25 anchored to the presynaptic membrane. It mediates fusion of synaptic vesicles with the presynaptic plasma membrane resulting in exocytosis of neurotransmitters into the synaptic cleft. While the gene...
ORGANISM(S): Rattus norvegicus (Rat) 
2023-01-25 | PXD030619 | Pride
Native mass spectrometry Chemical cross-linking Snare complex Complexin-1 Signal transmission in neurons 
ProteomicsProtein aggregation mediates protein complex stoichiometry in aneuploid cells, part 6
Aneuploidy, a condition characterized by chromosome gains and losses, causes reduced fitness and numerous cellular stresses, including increased protein aggregation. Here, we identify protein complex stoichiometry imbalances as a major cause of protein aggregation in aneuploid cells. Subunits of pro...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2020-05-26 | PXD011915 | Pride
Yeast Aggregates Proteostasis Aneuploidy 
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