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ProteomicsProteomic Ligandability Maps of Phosphorus(V) Stereoprobes Identify Covalent TLCD1 Inhibitors
Activity-based protein profiling (ABPP) of stereoisomerically defined sets of electrophilic compounds (‘stereoprobes’) offers a versatile way to discover covalent ligands for proteins in native biological systems. Here we report the synthesis and chemical proteomic characterization of stereoprobes b...
ORGANISM(S): Homo sapiens (Human) 
2026-09-14 | PXD060339 | Pride
Lipid biology Chemical biology Chemical proteomics Electrophilic probes 
ProteomicsA Global Ligandability Map of Tryptoline Butynamide Stereoprobes Identifies Covalent Inhibitors of the Actin Maturation Protease ACTMAP
Covalent chemistry coupled with activity-based protein profiling (ABPP) offers a versatile approach for small-molecule ligand discovery in native biological contexts. The covalent ligandability maps generated by ABPP that target cysteine have frequently leveraged the acrylamide as a reactive group d...
ORGANISM(S): Homo sapiens (Human) 
2026-05-20 | PXD074619 | Pride
ProteomicsProtein-directed ABPP describing proteome-wide reactivities of WX-02-520 and WX-02-521 probes
More than half of the ∼20,000 protein-encoding human genes have at least one paralog. Chemical proteomics has uncovered many electrophile-sensitive cysteines that are exclusive to a subset of paralogous proteins. Here, we explore whether such covalent compound-cysteine interactions can be used to di...
ORGANISM(S): Homo sapiens (Human) 
2024-09-03 | PXD053077 | Pride
Proteomics Chemical biology 
ProteomicsWild-type and O/E uPA in mouse aortic arches_PROTOMAP
Aortic arches from WT and overexpression of uPA were extracted and their proteome was processed and subjected to PROTOMAP analysis.
ORGANISM(S): Mus musculus (Mouse) 
2020-08-10 | PXD020406 | Pride
Protomap Aortic arches Upa 
ProteomicsPROTOMAP analysis of Human Cartoid Plaques
Human cartoid plaques were removed in operating room and placed in tube with PBS.Plaques were then analyzed for presence of rupture. The ulcerated lesicon was then excised and subjected to protein extraction. Areas of stable plaque tissue was then excised as a control.
ORGANISM(S): Homo sapiens (Human) 
2020-08-10 | PXD020405 | Pride
Human Plaques Protomap 
ProteomicsCysteine reactivity profiling identifies host regulators of Mycobacterium tuberculosis replication in human macrophages
Innate immune cells such as monocytes and macrophages provide the earliest defense against infection by intracellular pathogens by initiating signaling pathways and restricting pathogen replication. However, the full complement of proteins that mediate cell-autonomous immunity remains incompletely d...
ORGANISM(S): Homo sapiens (Human) 
2026-03-02 | PXD067709 | Pride
Innate immunity Cysteine Macrophage Mycobacterium tuberculosis Mass spectrometry Chemical proteomics Covalent probes 
ProteomicsSimple , Enantiocontrolled Azetidine Library Synthesis via Strain-Release Functionalization of 1-Azabicyclobutanes
Herein we describe a new enantioselective route for the synthesis of all stereoisomers of 2,3-disubstituted azetidines. This approach uses simple strain release functionalization of preformed 1-azabicyclobutanes (ABBs), which enables installation of a wide array of nucleophiles at the C3-position. C...
ORGANISM(S): Homo sapiens (Human) 
2026-06-29 | PXD055926 | Pride
Chemical synthesis Proteomics Covalent probes Chemical biology 
ProteomicsProteomic discovery of chemical probes that perturb protein complexes in human cells
Most proteins in the human proteome lack chemical probes, and several large-scale and generalizable small-molecule binding assays have been introduced to address this problem. How compounds discovered in such “binding-first” assays affect protein function, nonetheless, often remains unclear. Here, w...
ORGANISM(S): Homo sapiens (Human) 
2023-04-20 | PXD029655 | Pride
Human Size-exclusion Chemoproteomics Immunopeptidomics Covalent ligands 
ProteomicsCCNE1 interactome analysis in HEK293T cells under the treatment of different CCNE1(:CDK2) ligands
This is to explore potential functional effects of CCNE1(:CDK2) ligands and compare them side by side.
ORGANISM(S): Homo sapiens (Human) 
2024-09-03 | PXD048328 | Pride
Ip-ms 
ProteomicsRemodeling of oncogenic transcriptomes by small molecules targeting the RNA-binding protein NONO
A large swath of the human proteome is dedicated to mRNA homeostasis, but most RNA-binding proteins lack chemical probes1,2. Here, we report the discovery through phenotypic screening of electrophilic small molecules that swiftly (within 4 h) and stereospecifically decrease transcripts encoding the ...
ORGANISM(S): Homo sapiens (Human) 
2024-01-26 | PXD032087 | Pride
Human Lc-msms Tmt Cancer Chemoproteomics Thermo fusion 
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