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EMBL, SCB, Heidelberg, Germany (1)
Johns Hopkins University Assistant Professor, Department of Chemistry Assistant Professor, Department of Biology (by courtesy) Assistant Professor, Thomas C. Jenkins Department of Biophysics (by courtesy) Preceptor, Chemistry-Biology Interface (CBI) Program (1)
Johns Hopkins University, Chemistry (1)
Johns Hopkins University, Department of Chemistry, Johns Hopkins University, Baltimore, MD 21218, USA, Thomas C. Jenkins Department of Biophysics, Johns Hopkins University, Baltimore, MD 21218, USA (1)
Professor Department of Biology University of York York United Kingdom (1)
Professor in Translational Oncology Director of Postgraduate Teaching and Learning, School of Medicine Principal Investigator, Cancer Chemoradiation Research Group Department of Surgery, Trinity Translational Medicine Institute Trinity College Dublin, St. James’s Hospital, Dublin (1)
Professor in Translational Oncology Director of Postgraduate Teaching and Learning, School of Medicine Principal Investigator, Cancer Chemoradiation Research Group Department of Surgery, Trinity Translational Medicine Institute, Trinity St. James’s Cancer Institute, Rm 1.20, Trinity Centre for Health Sciences, Trinity College Dublin, St. James’s Hospital, Dublin 8, D08 W9RT, Ireland. (1)
Senior Lecturer School of Biological Sciences University Of East Anglia (1)
Stephen Fried Johns Hopkins University Fried Lab Chemistry Department (1)

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Nucleosome recognition and DNA distortion by the Chd1 remodeler revealed at 2.6 Å resolution

Chromatin remodelers are ATP-dependent enzymes that reorganize nucleosomes within all eukaryotic genomes. The Chd1 remodeler specializes in shifting nucleosomes into evenly spaced arrays, a defining characteristic of chromatin in gene bodies that blocks spurious transcription initiation. Linked to s...

ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) Xenopus laevis (African clawed frog)

2022-02-23 | PXD025287 | Pride

S. aureus gene expression during growth phase and/or S. aureusâ??PMN interaction

To measure S. aureus gene expression during growth phase and/or S. aureusâ??PMN interaction.

ORGANISM(S): Staphylococcus aureus

2010-06-09 | E-GEOD-2728 | biostudies-arrayexpress

An Intrinsically Disordered Region of Ubp10 Governs its Specificity for Deubiquitinating H2A/H2B Dimers over Nucleosomes

Histone modifications perform a vast array of functions in regulating gene expression, DNA replication, and repair. Monoubiquitination of histone H2B at K123 in yeast (K120 in humans) is an intriguing modification because it is deposited cotranscriptionally, mediates the installation of several othe...

ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast)

2025-07-16 | PXD051971 | Pride

Deubiqutinase Crosslinking mass spectrometry Ubiquitinated histone Ubp10 Single molecule fret Fact

ChIP-seq for LSM4 in U2OS-DIvA cells -/+ AsiSI-induction by 4OHT

Map LSM4 binding to chromatin at DNA double-strand breaks (DSBs) induced by AsiSI endonuclease in U2OS-DIvA cells.

ORGANISM(S): Homo sapiens

2026-04-30 | E-MTAB-16861 | biostudies-arrayexpress

Influenza Polymerase Can Adopt an Alternative Configuration Involving a Radical Repacking of PB2 Domains

Influenza virus polymerase transcribes or replicates the segmented RNA genome (vRNA) into respectively viral mRNA or full-length copies and initiates RNA synthesis by binding the conserved 3' and 5' vRNA ends (the promoter). In recent structures of promoter-bound polymerase, the cap-binding and endo...

ORGANISM(S): Influenza B virus (B/Memphis/13/03) Influenza A virus (A/Vietnam/1203/04(H5N1))

2016-01-04 | PXD002234 | Pride

Influenza Xl-ms Polymerase

Separation of DNA replication from the assembly of break-competent meiotic chromosomes

The meiotic cell division reduces the chromosome number from diploid to haploid to form gametes for sexual reproduction. Although much progress has been made in understanding meiotic recombination and the two meiotic divisions, the processes leading up to recombination, including the prolonged pre-m...

ORGANISM(S): Saccharomyces cerevisiae

2012-06-14 | E-GEOD-35667 | biostudies-arrayexpress

Genome-wide mapping of in vivo SCL/DNA interactions in erythroid cells

We have previously proposed two distinct molecular mechanisms by which SCL binds its targets in hematopoiesis; either by direct contact with specific DNA sequences or by indirect recruitment through interaction with other proteins. We have established that direct DNA binding is the major non-redund...

ORGANISM(S): Mus musculus

2010-05-18 | E-GEOD-18720 | biostudies-arrayexpress

Myb-domain protein Teb1 controls histone levels and centromere assembly in fission yeast

The TTAGGG motif is common to two seemingly unrelated dimensions of chromatin function M-^V the vertebrate telomere repeat and the promoter regions of many Schizosaccharomyces pombe genes, including all of those encoding canonical histones. The essential S. pombe protein Teb1 contains two Myb-like D...

ORGANISM(S): Schizosaccharomyces pombe

2013-08-14 | E-MTAB-1253 | biostudies-arrayexpress

ORC precisely positions nucleosomes at origins of replication

The budding yeast genome is marked by 250-350 origins of DNA replication. These origins are bound by the origin recognition complex (ORC) throughout the cell cycle. ORC has known DNA binding sequence preferences which, though necessary for binding, are not sufficient to fully specify a genomic locus...