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ProteomicsProteomic characterization of fresh spermatozoa and supernatant after cryopreservation in relation to freezability of carp (Cyprinus carpio) semen
Our recent studies suggested that the freezability of carp semen is related to seminal plasma protein profiles. Here, we aimed to compare the spermatozoa proteomes of good (GF) and poor (PF) freezability semen of carp. To achieve this, we used two-dimensional difference in gel electrophoresis follow...
ORGANISM(S): Cyprinus carpio (Common carp) 
2018-03-23 | PXD008187 | Pride
Cryoresilience Sperm Cryopreservation Fish Semen Proteome 
ProteomicsIn-depth proteomic analysis of carp (Cyprinus carpio L) spermatozoa
Using a combination of protein fractionation by one-dimensional gel electrophoresis and high performance liquid chromatography - electrospray ionization tandem mass spectrometry, we identified 348 proteins in carp spermatozoa, most of which were for the first time identified in fish. Dynein, tubulin...
ORGANISM(S): Cyprinus carpio (Common carp) 
2014-10-13 | PXD000877 | Pride
Carp Sperm Fish Proteome Lc-ms/ms 
ProteomicsApplication of Two-Dimensional Difference Gel Electrophoresis to Identify Protein Changes between Center, Margin, and Adjacent Non-Tumor Tissues Obtained from Non-Small-Cell Lung Cancer with Adenocarcinoma Subtype
Lung cancer is responsible for the most cancer-related mortality worldwide and the mechanism of its development is poorly understood. Proteomics has become a powerful tool offering vital knowledge related to cancer development. Using a two-dimensional difference gel electrophoresis (2D-DIGE) approa...
ORGANISM(S): Homo sapiens (Human) 
2022-05-20 | PXD032736 | Pride
Cancer Lung tissue Human 2d-dige Adenocarcinoma 
ProteomicsApplication of Two-Dimensional Difference Gel Electrophoresis to Identify Protein Changes between Center, Margin, and Adjacent Non-Tumor Tissues Obtained from Non-Small-Cell Lung Cancer with Squamous Cell Carcinoma Subtype
Lung cancer is responsible for the most cancer-related mortality worldwide and the mechanism of its development is poorly understood. Proteomics has become a powerful tool offering vital knowledge related to cancer development. Using a two-dimensional difference gel electrophoresis (2D-DIGE) approa...
ORGANISM(S): Homo sapiens (Human) 
2022-05-20 | PXD032962 | Pride
Cancer Tissue Lung Scc Human Squamous cell carcinoma 
ProteomicsHypoxia induces Foxn1 and Mmp-9 expression in keratinocytes: proteomics analysis of mouse keratinocytes transiently activated by Foxn1
Recent studies have shown that transcription factor Foxn1 expressed in keratinocytes is involved in skin wound healing process, yet how Foxn1 functions remains largely unknown. Proteomic analysis using a two-dimensional differential gel electrophoresis (2D-DIGE) technique coupled with MALDI-TOF/TOF ...
ORGANISM(S): Mus musculus (Mouse) 
2018-04-09 | PXD007843 | Pride
Proteomics Skin Wound healing Hypoxia Mmp-9 Foxn1 
ProteomicsHormonal stimulation of carp is accompanied with changes of seminal plasma and spermatozoa proteome
Hormonal stimulation in common carp is a routine practice to enhance sperm production and control gamete maturation. The mechanism regulating functional changes of semen composition upon hormone treatment is unknown at present. The aim of the study was to compare proteome of carp spermatozoa and sem...
ORGANISM(S): Cyprinus carpio (Common carp) 
2021-04-20 | PXD011049 | Pride
Semen Hormone Spermiation Fish Sperm maturation Proteome 
ProteomicsLoss of fertilizing ability of sturgeon spermatozoa cryopreserved with the use of DMSO is not related to impairment of sperm motility but alteration of proteins involved in acrosome reaction, egg recognition and early stages of embryo development
Background: The cryopreservation of semen is crucial for conserving genetic diversity and reproductive success in endangered species like the Siberian sturgeon. However, this process can cause cryodamage, affecting quality and protein profile of spermatozoa. While cryoprotectants like dimethyl sulfo...
ORGANISM(S): Acipenser baerii 
2024-08-10 | PXD052170 | Pride
Extracellular medium Mass spectrometry Cryopreservation Acipenser baerii Semen Proteome 
ProteomicsComparative proteomic analysis of eggs and ovarian fluid in Siberian sturgeon: Insights into composition and functional significance
Sturgeon species, considered living fossils, exhibit unique reproductive characteristics, making it crucial to comprehend the molecular processes governing the formation and quality of their eggs. However, there is a notable lack of comprehensive data concerning the protein composition analysis of s...
ORGANISM(S): Acipenser baerii 
2023-12-18 | PXD044168 | Pride
Mass spectrometry Acipenser baerii Ovarian fluid Eggs Proteome 
ProteomicsProteomic analysis of fresh and liquid stored turkey spermatozoa
The liquid storage of turkey semen without the loss of fertilizing ability is of practical interest to poultry industry. However, fertility rates from liquid stored turkey semen decline within a few hours. A clear cause of the decline in spermatozoa quality is unknown. Therefore, the purpose of the ...
ORGANISM(S): Meleagris gallopavo (Common turkey) 
2024-01-26 | PXD043050 | Pride
Turkey Maldi tof/tof 2d-dige Spermatozoa 
ProteomicsProteomic analysis of fresh, equilibrated, and frozen/thawed turkey semen in relation to the extracellular fluid proteome
The cryopreservation of avian semen without significant loss of fertilizing ability is of practical interest to the poultry industry and for the conservation of genetic biodiversity. However, the unique physiological features make avian spermatozoa highly sensitive to damage caused by cryopreservati...
ORGANISM(S): Meleagris gallopavo (Common turkey) 
2025-07-08 | PXD051615 | Pride
Turkey Cryopreservation Semen 
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