Sort   by:  
 Page size 
MetabolomicsMetabolic responses induced by DNA damage and poly (ADP-ribose) polymerase (PARP) inhibition in MCF-7 cells
Genomic instability is one of the hallmarks of cancer. Several chemotherapeutic drugs and radiotherapy induce DNA damage to prevent cancer cell replication. Cells in turn activate different DNA damage response (DDR) pathways to either repair the damage or induce cell death. These DDR pathways also e...
2015-08-01 | MTBLS177 | MetaboLights
MetabolomicsThe Poly (ADP-Ribose) Polymerase inhibitor Veliparib and Radiation Cause Significant Cell Line Dependent Metabolic Changes in Breast Cancer Cells
Breast tumors are characterized into different subtypes based on their surface marker expression, which affects their prognosis and treatment. For example, triple negative breast cancer cells (ER-/PR-/Her2-) show reduced susceptibility towards radiotherapy and chemotherapeutic agents. Poly (ADP-ribo...
2016-11-07 | MTBLS337 | MetaboLights
MetabolomicsA synthetic cell with integrated DNA self-replication and lipid biosynthesis

The emergence, organization, and persistence of cellular life are the result of the functional integration of metabolic and genetic networks. Here, we engineer phospholipid vesicles that can operate three essential functions, namely transcription-translation of a partial genome, self-replication ...

2026-02-02 | MTBLS13437 | MetaboLights
ProteomicsT7 DNA Polymerase with fluorescent unnatural amino acid
A T7 DNA polymerase variant with the fluorescent unnatural amino acid (7-hydroxycoumarin-4-yl) ethylglycine (7-HCou) incorporated at position 514 was expressed in E. coli and purified. The protein was then characterized by mass spec, pre-steady state kinetic experiments, and fluorescence measurement...
ORGANISM(S): Escherichia virus T7 
2020-10-19 | PXD021676 | Pride
T7 Unnatural amino acid Dna polymerase 7-hydroxycoumarin-4-yl ethylglycine 
ProteomicsPAXX and its paralogues synergistically direct DNA polymerase I activity in DNA repair
In this study, we characterised the interactomes of PAXX and its paralogs, XLF and XRCC4, to show that these factors share the ability to interact with DNA polymerase lambda (Poll), stimulate its activityand are required for the recruitment of Poll to laser-induced DNA damage sites.
ORGANISM(S): Homo sapiens (Human) 
2018-09-24 | PXD010891 | Pride
Polymerase lambda Xrcc4 Double strand breaks Nhej Paxx Dna repair Xlf Dna-pkcs 
UnknownMutants of Taq DNA polymerase resistant to PCR inhibitors allow DNA amplification from whole blood and crude soil samples.
Not available
S-EPMC2655666 | biostudies-literature
ProteomicsPARG is Essential for Pol-theta-Mediated DNA End-Joining by Removing Repressive Poly-ADP Ribose Marks
DNA polymerase theta (Pol-theta)-mediated end-joining (TMEJ) repairs DNA double-strand breaks and confers resistance to genotoxic agents. How Pol-theta is regulated at the molecular level to exert TMEJ remains unknown. We find that Pol-theta interacts with and is PARylated by PARP1 in vitro and in c...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
MSV000092839 | MassIVE
GenomicsDNA polymerase and mismatch repair deficient cancers exert distinct genome-wide microsatellite signatures 2
Whole genome sequencing data for the manuscript: DNA polymerase and mismatch repair deficient cancers exert distinct genome-wide microsatellite signatures
EGAS00001004816 | EGA
TranscriptomicsMaintenance of Long-Range DNA Interactions after Inhibition of Ongoing RNA Polymerase II Transcription.
A relationship exists between nuclear architecture and gene activity and it has been proposed that the activity of ongoing RNA polymerase II transcription determines genome organization in the mammalian cell nucleus. Recently developed 3C and 4C technology allowed us to test the importance of transc...
ORGANISM(S): Mus musculus 
2010-05-26 | E-GEOD-10170 | biostudies-arrayexpress
UnknownLive culture-based qPCR screening of Taq DNA polymerase variants for resistance to PCR inhibitors.
Not available
S-EPMC12425957 | biostudies-literature
Sort   by:  
 Page size