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MetabolomicsDNA-PKcs drives CKD progression by activating TAF7/RAPTOR/mTORC1 signaling-mediated metabolic reprogramming

Kidney injury initiates epithelial dedifferentiation and myofibroblast activation during the progression of chronic kidney disease (CKD). Herein, we found that the expression of DNA-dependent protein kinase catalytic subunit (DNA-PKcs) was significantly increased in the kidney tissues of both CKD...

2023-01-25 | MTBLS5971 | MetaboLights
ProteomicsIntegrative Threading of the DNA-PKcs Disordered Region with Crosslinking Restraints
Integrative structure modelling was applied to understand how auto-phosphorylation of the ABCDE cluster in DNA-PKcs can occur. We used crosslinking restraints to assign sequence to structurally unassigned helices in DNA-PKcs s in the largely disordered region containing the ABCDE cluster. Our final ...
ORGANISM(S): Homo sapiens (Human) 
2019-12-10 | PXD016639 | Pride
Crosslinking-ms Integrative modelling Dna-pkcs 
ProteomicsKinetic Analysis of DNA Dependent Protein Kinase Catalytic Subunit
We investigated the dynamics of protein kinase DNA dependent protein kinase catalytic subunit (DNA-PKcs) through hydrogen deuterium exchange mass spectrometry. Over 1100 peptides could be monitored throughout the kinetics experiment, showing the utility of our nanospray HX-MS configuration for ultr...
ORGANISM(S): Homo sapiens (Human) 
2019-12-10 | PXD016595 | Pride
Hdx-ms Nanospray hx-ms Human Dna-pkcs 
ProteomicsDNA-PKcs Interacts with Fis1 Induce Mitochondrial Fragmentation During Acute Kidney Injury
To analyze the mechanisms underlying DNA-PKcs deletion-mediated renoprotection in an unbiased fashion, we performed proteomic analysis using label free technology to obtain the protein expression profiles in control and DNA-PKcs-deficient mice subjected to septic AKI. 631 proteins were altered by DN...
ORGANISM(S): Acyrthosiphon Pisum 
2021-12-08 | PXD030271 |
Proteomicsmass spectrometry analysis of in vitro kinase assay for DNA-PKcs and TAF7
To examine whether TAF7 could be phosphorylated by DNA-PKcs directly. An in vitro kinase assays were performed. Firstly, human DNA-PKcs was purified from TGF1 treated HK2 cells by immunoprecipitation with a DNA-PKcs antibody (abcam) and IgG was used as a negative control. The purified proteins and ...
ORGANISM(S): Homo Sapiens 
PXD036930 |
ProteomicsER-associated degradation ligase HRD1 links ER stress to DNA damage repair by modulating the activity of DNA-PKcs
ER-associated degradation ligase HRD1 links ER stress to DNA damage repair by modulating the activity of DNA-PKcs
ORGANISM(S): Homo Sapiens 
PXD055020 |
GenomicsDNA-PKcs Suppresses Illegitimate Chromosome Rearrangements
DNA-PKcs Suppresses Illegitimate Chromosome Rearrangements
PRJNA1049124 | ENA
ProteomicsIntegrative Structural Model of DNA-PK Synapsis
In non-homologous end joining repair of DNA double strand breaks, DNA dependent protein kinase catalytic subunit (DNA-PKcs) and Ku70/80 binds the free DNA ends forming the holoenzyme DNA-PK. DNA-PK synapses across the break to tether the broken ends in the initial long range synaptic complex. We gen...
ORGANISM(S): Homo sapiens (Human) 
2021-01-20 | PXD017931 | Pride
Dna-pk Hydrogen deuterium exchange Crosslinking 
GenomicsDNA-PKcs suppress chromosome translocation in human cells
DNA-PKcs suppress chromosome translocation in human cells
PRJNA974458 | ENA
ProteomicsDNA-PKcs kinase activity stabilizes the transcription factor Egr1 in activated immune cells
To gain insight into the function of DNA-PKcs within immune cells, we performed a quantitative phosphoproteomic screen in T cells to identify first order phosphorylation targets of DNA-PKcs. Results indicate that DNA-PKcs phosphorylates the transcription factor Egr1 (early growth response protein 1)...
ORGANISM(S): Homo sapiens (Human) 
2021-12-21 | PXD026352 | Pride
Ms3 Phospo Tmt 
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