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ProteomicsNIH 3T3 AHA p-c, SILAC p-c, inhibitor, and enrichment control experiments
We developed a pulse-chase method in where fully SILAC (heavy, medium-heavy and Light) labelled cells were pulsed with Azidohomoalanine (AHA) and then chased for different length of times. These experiments were performed with 0, 1, 2, 4, 8, 16 and 13 h of chase. Data from 3 biological replicates ar...
ORGANISM(S): Mus musculus (Mouse) 
2016-10-06 | PXD004929 | Pride
Azidohomoalanine Mouse Silac Degradation Aneuploidy 
UnknownP-body proteins regulate transcriptional rewiring to promote DNA replication stress resistance.
Not available
S-EPMC5601920 | biostudies-literature
UnknownCopper bis(thiosemicarbazone) complexes modulate P-glycoprotein expression and function in human brain microvascular endothelial cells.
Not available
S-EPMC9540023 | biostudies-literature
ProteomicsNovel Kinesin Family 1A Variants Linked to Atypical Parkinsonism Elicit Altered Neuronal TDP-43 Interactions and Dendritic Atrophy
We used patient induced pluripotent stem cells (IPSC) derived neurons from a patient with two mutations in KIF1A to evaluate the quantitative changes to KIF1A protein interaction, relative to wild-type control IPSC-motor neurons, using an anti-KIF1A antibody during immunoprecipitation. Given the rel...
ORGANISM(S): Homo sapiens (Human) 
2025-08-26 | PXD067234 | Pride
Kif1a Ipsc motor neurons Tdp-43 
UnknownPre-concentration by liquid intake by paper (P-CLIP): a new technique for large volumes and digital microfluidics.
Not available
S-EPMC7734381 | biostudies-literature
ProteomicsRPE-1 and RPE-1 trisomic AHA p-c and steady state data
We developed a pulse-chase method in where fully SILAC (heavy, medium-heavy and Light) labelled cells were pulsed with Azidohomoalanine (AHA) and then chased for different length of times. In addition, steady state protein levels comparing the RPE- and RPE-1 trisomic cells by standard SILAC labeling...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2017-03-31 | MSV000080831 | MassIVE
ProteomicsGlobal Landscape of Cell Envelope Protein Complexes in Escherichia coli
After extraction with mild non-denaturing detergents, we affinity-purified 785 endogenously-tagged CEPs and then identified stably-associated polypeptides by precision mass spectrometry. The resulting high-quality physical interaction network, comprising most (77%) of all targeted CEPs, revealed hu...
ORGANISM(S): Escherichia coli (strain K12 / W3110 / ATCC 27325 / DSM 5911) 
2017-11-30 | PXD006247 | Pride
Escherichia coli Multi-protein complexes Macromolecues Protein-protein interactions Cell envelope Cell envelope proteins Cell envelope protein complexes 
ProteomicsRPE-1 and RPE-1 trisomic AHA p-c and steady state data
We developed a pulse-chase method in where fully SILAC (heavy, medium-heavy and Light) labelled cells were pulsed with Azidohomoalanine (AHA) and then chased for different length of times. In addition, steady state protein levels comparing the RPE- and RPE-1 trisomic cells by standard SILAC labeling...
ORGANISM(S): Homo sapiens (Human) 
2016-10-06 | PXD004915 | Pride
Human Aneuploidy Pre-1 
TranscriptomicsMicroarray and RNAi Analysis of P450s in Anopheles gambiae Male and Female Steroidogenic Tissues: CYP307A1 is Required for Ecdysteroid Synthesis
Small-scale microarray profiling of all the genes encoding P450 enzymes of the malaria mosquito Anopheles gambiae in active steroidogenic organs of adults. Ovaries from non blood-fed females were compared to ovaries of blood-fed females at different times after the blood meal: 16 and 22h post-blood-...
ORGANISM(S): Anopheles gambiae 
2013-09-10 | E-MTAB-1697 | biostudies-arrayexpress
UnknownRecognition of Semaphorin Proteins by P. sordellii Lethal Toxin Reveals Principles of Receptor Specificity in Clostridial Toxins.
Not available
S-EPMC7316060 | biostudies-literature
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