Sort   by:  
 Page size 
ProteomicsTime-resolved analysis of proteome dynamics by TMT-SILAC hyperplexing
In this project, a more efficient methodology for obtaining multi-timepoint kinetic data for proteome-wide analyses of protein turnover is developed and used to globally measure the kinetics of protein turnover in primary human fibroblasts (HCA2-hTert). This approach takes advantage of the multiplex...
ORGANISM(S): Homo sapiens (Human) 
2016-12-14 | PXD004725 | Pride
Tmt-silac Protein degrdation Tmt Proteome dynamics Silac Human primary fibroblasts Protein synthesis Hyperplexing 
ProteomicsSILAC-based proteomic quantification of chemoattractant induced cytoskeleton dynamics on a second to minute timescale
Cytoskeletal dynamics during cell behaviours ranging from endocytosis and exocytosis to cell division and movement is controlled by a complex network of signalling pathways, the full details of which are as yet unresolved. Here we show that SILAC-based proteomic methods can be used to characterise t...
ORGANISM(S): Dictyostelium discoideum (Slime mold) 
2014-02-26 | PXD000738 | Pride
Cytoskeleton Silac 
ProteomicsNeuronal proteome dynamics during homeostatic scaling.
Homeostatic scaling adjusts synaptic strength in response to persistent changes in neuronal network activity. This compensatory mechanism requires proteome remodeling accomplished via regulation of protein synthesis as well as degradation, but the global patterns of proteome remodeling and the under...
ORGANISM(S): Rattus norvegicus (Rat) 
2020-03-31 | PXD016004 | Pride
Synaptic plasticity Proteome turnover Dynamic silac Proteome remodelling Homeostatic scaling 
UnknownDevelopment of a 5-plex SILAC method tuned for the quantitation of tyrosine phosphorylation dynamics.
Not available
S-EPMC3820944 | biostudies-literature
ProteomicsAnalysis of signaling endosome composition and dynamics using SILAC in embryonic stem cell-derived neurons
Neurons require efficient transport mechanisms such as fast axonal transport to ensure neuronal homeostasis and survival. Neurotrophins and their receptors are conveyed via fast axonal retrograde transport of signaling endosomes to the soma, where they elicit transcriptional responses. Despite the e...
ORGANISM(S): Mus musculus (Mouse) 
2015-12-21 | PXD002111 | Pride
Mass spectrometry Silac Motor neurons Signalling endosomes 
ProteomicsDynamics of protein expression reveals primary targets and secondary messengers of estrogen receptor signaling in MCF-7 breast cancer cells
Estrogen receptor-mediated proliferation of breast cancer cells is facilitated through expression of multiple primary target genes, products of which induce the secondary response to stimulation. To differentiate between the primary and secondary target genes of estrogen receptor signaling, we measu...
ORGANISM(S): Homo sapiens (Human) 
2016-04-14 | PXD002797 | Pride
Estradiol Srm Estrogen receptor Ere Silac Estrogen-regulated proteins Mcf-7 
UnknownSILAC-based proteomic quantification of chemoattractant-induced cytoskeleton dynamics on a second to minute timescale.
Not available
S-EPMC3971484 | biostudies-literature
ProteomicsProteome dynamics in iPSC-derived human dopaminergic neurons – part 2: protein turnover dynamics using SILAC
Dopaminergic neurons participate in fundamental physiological processes and are the cell type primarily affected in Parkinson’s disease (PD). Their analysis is challenging due to the intricate nature of their function, their involvement in diverse neurological processes, their heterogeneity and loca...
ORGANISM(S): Homo sapiens (Human) 
2024-09-12 | PXD051015 | Pride
Proteomics Ipsc Axon Dopaminergic neurons Protein turnover 
UnknownCombining Data Independent Acquisition With Spike-In SILAC (DIA-SiS) Improves Proteome Coverage and Quantification.
Not available
S-EPMC11795695 | biostudies-literature
ProteomicsSILAC-Tp (12 importins) - Extensive cargo identification reveals distinct biological roles of the 12 importin pathways
Importin-beta family proteins are nucleocytoplasmic transport receptors (NTRs), and they transport most nuclear proteins as their cargoes into and out of the nuclei. Human cells have 20 species of importin-beta family NTRs, of which 10 (importin-beta, transportin-1, -2, -SR, importin-4, -5, -7, -8, ...
ORGANISM(S): Homo sapiens (Human) 
2017-01-20 | PXD004655 | Pride
Nuclear transport Cargo Silac Transportin Importin Human Lc-ms/ms 
Sort   by:  
 Page size