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TranscriptomicsCharacterization of human malaria parasite Plasmodium falciparum eIF4E homologue and mRNA 5′ cap status
The mRNA 5′ cap is normally essential for eukaryotic mRNA translation, stabilization and transport and both the cap and eIF4E are important elements of post-transcriptional gene regulation. To further our understanding of mRNA translation in the human malaria parasite Plasmodium falciparum, we hav...
ORGANISM(S): Plasmodium falciparum 
2010-06-10 | E-GEOD-6356 | biostudies-arrayexpress
TranscriptomicsMDA-MB-231 eIF4E RIP-CHIP
Cytoplasmic RNA bound to eIF4E was pulled down from MDA-MB-231 cells to determine the influence of radiation on eIF4E mRNA binding 4 samples were analyzed with 3 biological replicates
ORGANISM(S): Homo sapiens 
2012-06-14 | E-GEOD-36179 | biostudies-arrayexpress
TranscriptomicseIF4E phosphorylation promotes tumorigenesis and is associated with prostate cancer progression
Translational regulation plays a critical role in cell growth and proliferation, and its dysregulation results in cancer. Aberrant expression of the mRNA 5’cap-binding protein, eIF4E, has been implicated in cancer development and progression. eIF4E activity is promoted by phosphorylation. Here we sh...
ORGANISM(S): Mus musculus 
2010-08-17 | E-GEOD-17451 | biostudies-arrayexpress
TranscriptomicsRedefining the role of eIF4E dose in development, cancer, and protein synthesis
eIF4E, the major cap-binding protein, has long been considered limiting for translating the mammalian genome. However, the requirement for eIF4E dose at an organismal level remains unexplored. By generating an Eif4e haploinsufficient mouse, we surprisingly found that 50% reduction in eIF4E, while co...
ORGANISM(S): Mus musculus 
2015-07-01 | E-GEOD-68238 | biostudies-arrayexpress
TranscriptomicsTranslational control of cell viability downstreams of eIF4E, using polyribosome RNA
Aberrant activation of the translation initiation machinery is a common property of malignant cells, and is essential for breast carcinoma cells to manifest a malignant phenotype. How does sustained activation of the rate limiting step in protein synthesis so fundamentally alter a cell? In this repo...
ORGANISM(S): Mus musculus 
2010-06-10 | E-GEOD-2094 | biostudies-arrayexpress
TranscriptomicsEpigenetic activation of a subset of mRNAs identified using polyribosome microarray analysis
Background: Translation deregulation is an important mechanism that causes aberrant cell growth, proliferation and survival. eIF4E, the mRNA 5 prime capâ??binding protein, plays a major role in translational control. To understand how eIF4E affects cellular proliferation and cell survival, we identi...
ORGANISM(S): Mus musculus 
2010-06-11 | E-GEOD-6639 | biostudies-arrayexpress
ProteomicsLC-MS analysis of Murine eIF4E-BP1/2 Double Knockout naive and TCR stimulated CD4+ T cells
This project focuses on examining the differences in the global CD4+ T cell proteome between cells lacking the negative regulators of eIF4E( eIf4E-Binding Proteins 1/2) and WT naive CD4+ T cells both prior and following TCR stimulation.
ORGANISM(S): Mus musculus (Mouse) 
2023-05-20 | PXD039635 | Pride
Translational regulation Immunology Cd4+ t cells Murine Eif4e 
ProteomicsProteome profiling followng targeting eIF4E
Here we have compared the impact of loss the protein synthesis initiation factor eIF4e in non samll cell lung cancer cells. Expression of eIF4E was targeted by an eIF4E siRNA or by expressiojn of eIF4E-dTAG construct allowing degradation of eIF4e-dTAG with the heterobifunctional dTAGv-1 small molecu...
ORGANISM(S): Homo sapiens (Human) 
2025-05-07 | PXD057122 | Pride
Human Cancer Lung Dtag Protein synthesis Eif4e Sirna 
TranscriptomicsTranslation State Array Assay for C elegans IFE-1-dependent mRNAs
Relative polysomal loading changes for wild type (N2) versus ife-1(bn127) C. elegans strains Strain ife-1(bn127) is null for the gene encoding one of five eIF4E isoforms in the worm, IFE-1. Only this isoform associates with P granules. Resolution of translating and non-translating mRNAs from WT and...
ORGANISM(S): Caenorhabditis elegans 
2016-01-07 | E-GEOD-74459 | biostudies-arrayexpress
ProteomicsChlorocebus sabaeus vero E6 and and eIF4E(S209A) Vero cells (S209A-Vero) LC-MSMS upon infection by coronavirus PEDV
Two rounds of TMT relative quantitative proteomics were performed to detect cellular factors involved in p-eIF4E regulation of the synthesis of viral proteins.our first round of screening identified differentially expressed proteins in PEDV-infected cells and mock-infected cells; the cellular pathwa...
ORGANISM(S): Cercopithecus aethiops (Green monkey) (Grivet) 
2023-03-10 | PXD033916 | Pride
Chlorocebus sabaeus vero e6 Pedv And eif4e(s209a) vero cells (s209a-vero) Phosphorylation of eif4e Lc-msms 
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