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MetabolomicsStress-induced enrichment of Pseudomonas sp. stimulates the adaptive response of Auxenochlorella pyrenoidosa and antibiotic-resistant proliferation

The phycosphere is an important ecological niche for bacteria and antibiotic resistance genes (ARGs). However, whether and how the interaction between microalgae and bacteria changed, and its further effect on the transmission of ARGs under pollutant stress remains enigmatic. Here, Auxenochlorell...

2025-09-26 | MTBLS11371 | MetaboLights
MetabolomicsBacillus licheniformis promotes the enrichment of Prevotella improving levels of nicotinamide in alleviating colitis

This study investigated the effects of Bacillus licheniformis supplementation on the gut metabolic profile of patients with mild-to-moderate active ulcerative colitis (UC). Patients received either mesalazine alone or mesalazine combined with Bacillus licheniformis. Fecal samples were collected a...

2026-09-11 | MTBLS15626 | MetaboLights
ProteomicsSystematic comparison of strategies for the enrichment of lysosomes by data independent acquisition
We describe a comparison of individual approaches for the enrichment of lysosomes comparing four different strategies using data independent acquisition (DIA). As a benchmark, we chose a crude enrichment strategy, which is the centrifugation of the PNS at 20,000 x g termed organelle enriched pellet ...
ORGANISM(S): Homo sapiens (Human) 
2020-01-21 | PXD015098 | Pride
Enrichment methods Lysosomes Data independent acquisition (dia) 
ProteomicsAn Internal Standard for Assessing Phosphopeptide Recovery from Metal Ion/Oxide Enrichment Strategies
Phosphorylation-mediated signaling pathways have major implications in cellular regulation and disease. Mass spectrometry-based proteomics is a well-established approach for quantifying thousands of phosphorylation events in a single experiment. However, proteins with roles in signaling pathways are...
ORGANISM(S): Homo sapiens (Human) Saccharomyces cerevisiae (Baker's yeast) 
2018-12-04 | PXD008966 | Pride
Tmt super-heavy Lumos Phosphorylation Mtraq Tmtzero Tmtsh Tmt0 
ProteomicsImproving quantitative glycoproteomics by systematic evaluation of different glycopeptide enrichment, quantification, and mass spectrometry strategies
In this study, we conducted a systematic investigation into a glycoproteomic workflow. Firstly, Label-free and TMT labeling strategies were utilized for intact N-glycopeptide quantification. A total of 2,924 unique intact N-glycopeptides can be quantified after being labeled using TMT approach. Comp...
ORGANISM(S): Homo sapiens (Human) Mus musculus (Mouse) 
2025-05-07 | PXD054874 | Pride
Glycoproteomics Intact glycopeptide Identification Quantification 
ProteomicsEnrichment strategy for searching missing protein
Enrichment strategy for searching missing protein is a project under the guidance of Chromosome-Centric Human Proteome Project (C-HPP). We developed systematical enrichment strategies for revealing the existence of MPs including low molecular weight (LMW), membrane proteins, post-translational modif...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2019-06-12 | MSV000083957 | MassIVE
ProteomicsCharacterization of phosphopeptide enrichment strategy for mass spectrometry-based proteomic analysis
We attempted to evaluate four strategies for phosphopeptide enrichment, using the titanium dioxide (TiO2) and Phos-tag ligand particles, from in-solution or in-gel digests prior to mass spectrometry-based proteomic analysis.
ORGANISM(S): Homo Sapiens (human) Cellular Organisms 
2021-12-24 | PXD026295 | JPOST Repository
ProteomicsEnrichment strategy for searching missing protein
Enrichment strategy for searching missing protein is a project under the guidance of Chromosome-Centric Human Proteome Project (C-HPP). We developed systematical enrichment strategies for revealing the existence of MPs including low molecular weight (LMW), membrane proteins, post-translational modif...
ORGANISM(S): Homo sapiens (Human) 
2015-07-14 | PXD002255 | Pride
Missing protein Chpp Enrichment strategy 
ProteomicsA comparison of blood plasma exosome enrichment strategies for proteomic analysis.
Proteomic analysis of exosomes (EXs) poses a significant challenge. A ‘gold-standard’ method for plasma EX enrichment for downstream proteomic analysis is yet to be established. Methods were evaluated for their capacity to successfully isolate and enrich EXs from plasma, minimise the presence of hig...
ORGANISM(S): Bos taurus (Bovine) 
2022-08-12 | PXD032313 | Pride
Exosome  isolation  enrichment  ultracentrifugation  size-exclusion chromatography  ultrafiltration  mass-spectrometry  proteomics  extracellular vesicles 
ProteomicsEvaluating Protein Enrichment Methods to Improve Biomarker Discovery in Equine Neurological Disorders
This study evaluated and compared three sample preparation strategies for proteomic analysis of equine cerebrospinal fluid (CSF): native in-solution digestion, the ProteoMiner™ Small-Capacity Enrichment Kit (Bio-Rad®), and the PreOmics Enrich-iST™ Kit (PreOmics®). CSF is in direct contact with the c...
ORGANISM(S): Equus caballus (Horse) 
2026-04-13 | PXD063239 | Pride
Cerebrospinal fluid (csf)  proteomics  native digestion  proteominer™ small-capacity enrichment kit  preomics enrich-ist™  liquid chromatography with tandem mass spectrometry (lc-ms/ms) 
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