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ProteomicsAn Internal Standard for Assessing Phosphopeptide Recovery from Metal Ion/Oxide Enrichment Strategies
Phosphorylation-mediated signaling pathways have major implications in cellular regulation and disease. Mass spectrometry-based proteomics is a well-established approach for quantifying thousands of phosphorylation events in a single experiment. However, proteins with roles in signaling pathways are...
ORGANISM(S): Homo sapiens (Human) Saccharomyces cerevisiae (Baker's yeast) 
2018-12-04 | PXD008966 | Pride
Tmt super-heavy Lumos Phosphorylation Mtraq Tmtzero Tmtsh Tmt0 
ProteomicsRemoval of NHS-labelling by-products in proteomic samples
N-Hydroxysuccinimide (NHS) chemistry is used extensively across proteomics sample preparation. One of the most popular applications is its use in isobaric reagent based quantitation; specifically both the iTRAQ and TMT approaches. The labels are introduced onto primary Serines, tyrosines, and threon...
ORGANISM(S): Homo sapiens (Human) 
2025-03-14 | PXD054730 | Pride
Nhs Methylamine Hydroxylamine N-hydroxysuccinimide O-acylation 
UnknownmTMT: An Alternative, Nonisobaric, Tandem Mass Tag Allowing for Precursor-Based Quantification.
Not available
S-EPMC7294932 | biostudies-literature
UnknownAn Internal Standard for Assessing Phosphopeptide Recovery from Metal Ion/Oxide Enrichment Strategies.
Not available
S-EPMC6004253 | biostudies-literature
ProteomicsmTMT: An alternative, non-isobaric, tandem mass tag allowing for precursor-based quantification
Stable isotope labeling of peptides is the basis for numerous mass spectrometry-based quantification strategies. Isobaric tagging and metabolic labeling, namely TMT and SILAC, are among the most widely used techniques for relative protein quantification. Here we report an alternative, precursor-base...
ORGANISM(S): Homo sapiens (Human) 
2019-09-16 | PXD014545 | Pride
Silac Tmt Isobaric Sh-sy5y Svgp12 Metabolic 
ProteomicsOptimization of Higher-Energy Collisional Dissociation Fragmentation Energies for Intact Protein-level Tandem Mass Tag Labeling
Isobaric chemical tag labeling (e.g., iTRAQ and TMT) is a commonly used approach in quantitative proteomics research. Typically, peptides are covalently labeled with isobaric chemical tags, and quantification is enabled through detection of low-mass reporter ions generated after MS2 fragmentation. R...
ORGANISM(S): Homo sapiens (Human) 
2023-01-17 | PXD037491 | Pride
Protein-level tmt labeling  top-down proteomics  quantitative proteomics  hcd 
ProteomicsProteomic Analysis of Human Brown Adipose Tissue Reveals Utilization of Coupled and Uncoupled Energy Expenditure Pathways
Human brown adipose tissue (BAT) has become an attractive target to combat the current epidemical spread of obesity and its associated co-morbidities. Currently, information on its functional role is primarily derived from rodent studies. Here, we present the first comparative proteotype analysis of...
ORGANISM(S): Homo sapiens (Human) 
2016-07-21 | PXD003843 | Pride
Human Adipose tissue 
ProteomicsS-Nitrosylation of the Virulence Regulator AphB Promotes Vibrio cholerae Pathogenesis
S-Nitrosylation of the Virulence Regulator AphB Promotes Vibrio cholerae Pathogenesis
ORGANISM(S): Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961) Bacteria 
2022-05-31 | PXD031615 | Pride
Virulence Reactive nitrogen species Hmpa Colonization Tcpp Vibrio cholerae Aphb 
ProteomicsTMT labeling for the masses: A robust and cost-efficient, in-solution labeling strategy
Isobaric stable isotope labeling using, for example, tandem mass tags (TMTs) is increasingly being applied for large-scale proteomic studies. Experiments focusing on proteoform analysis in drug time course or perturbation studies or in large patient cohorts greatly benefit from the reproducible quan...
ORGANISM(S): Homo sapiens (Human) Mus musculus (Mouse) 
2020-01-14 | PXD012703 | Pride
Stable isotope labeling Tandem mass tags Nhs ester chemistry Labeling efficiency 
ProteomicsProteomic Profiles of Human Arterioles Isolated from Fresh Adipose Tissue or Following Overnight Storage
Arterioles are key determinants of the total peripheral vascular resistance, which, in turn, is a key determinant of arterial blood pressure. However, the amount of protein available from one isolated human arteriole may be less than 5µg, making proteomic analysis challenging. In addition, obtaining...
ORGANISM(S): Homo sapiens (Human) 
2024-03-21 | PXD044454 | Pride
Arterioles Tmt labeled mass spectrometry Booster channel Overnight storage Proteomics 
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