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ProteomicsmTMT: An alternative, non-isobaric, tandem mass tag allowing for precursor-based quantification
Stable isotope labeling of peptides is the basis for numerous mass spectrometry-based quantification strategies. Isobaric tagging and metabolic labeling, namely TMT and SILAC, are among the most widely used techniques for relative protein quantification. Here we report an alternative, precursor-base...
ORGANISM(S): Homo sapiens (Human) 
2019-09-16 | PXD014545 | Pride
Silac Tmt Isobaric Sh-sy5y Svgp12 Metabolic 
ProteomicsCharacterisation of the protein cargo of murine plasma microparticles released during experimental cerebral malaria
Cerebral malaria (CM) is a severe complication of a Plasmodium falciparum infection and is still responsible for a high number of deaths and long term disabilities in children in sub-Saharan Africa. The pathogenesis of this syndrome remains incompletely understood but a number of mechanisms and effe...
ORGANISM(S): Mus musculus (Mouse) 
2016-12-13 | PXD003772 | Pride
Qexactive orbitrap Protein cargo Tandem mass tag (tmt) Microparticles Experimental cerebral malaria 
UnknownmTMT: An Alternative, Nonisobaric, Tandem Mass Tag Allowing for Precursor-Based Quantification.
Not available
S-EPMC7294932 | biostudies-literature
ProteomicsOptimized NHS-amine labeling for multiplexed phosphoproteome study.
In this project, we report the detrimental effect of hydroxylamine, a quenching reagent for NHS-amine reaction, on phosphopeptides. The degree of phosphopeptide enrichment was greatly improved by a desalting method compared with a vacuum-drying method right after quenching reaction. We demonstrate t...
ORGANISM(S): Homo sapiens (Human) 
2018-02-27 | PXD008082 | Pride
Phosphoproteome Β-elimination Hydroxylamine Phosphorylation Tandem mass tag N-hydroxysuccinamide-esters Isobaric tag 
ProteomicsThe small RNAs PA2952.1 and PrrH as regulators of virulence, motility and iron metabolism in Pseudomonas aeruginosa
Pseudomonas aeruginosa is a Gram-negative opportunistic pathogen that undergoes swarming motility in response to semisolid conditions with amino acids as a nitrogen source. With a genome encoding hundreds of potential intergenic small RNAs (sRNAs), P. aeruginosa can easily adapt to different conditi...
ORGANISM(S): Pseudomonas Aeruginosa 
2020-08-15 | MSV000085955 | MassIVE
ProteomicsInfluence of sRNA PA0805.1 on motility and virulence
Pseudomonas aeruginosa is a motile species that initiates swarming motility in response to specific environmental cues. The small non-coding RNA sRNA PA0805.1 was shown to be 5-fold upregulated under swarming conditions. This sRNA was cloned, transformed into PAO1 WT and overexpressed, leading to b...
ORGANISM(S): Pseudomonas Aeruginosa Pao1 (ncbitaxon:208964) 
MSV000084373 | MassIVE
ProteomicsEvaluation and Optimization of High-Field Asymmetric Waveform Ion Mobility Spectrometry for Multiplexed Quantitative Site-specific N-glycoproteomics
The heterogeneity and complexity of glycosylation hinder the depth of site-specific glycoproteomics analysis. High-field asymmetric-waveform ion-mobility spectrometry (FAIMS) has shown to improve the scope of bottom-up proteomics. The benefits of FAIMS for quantitative N-glycoproteomics have not bee...
ORGANISM(S): Homo sapiens (Human) 
2021-05-05 | PXD023951 | Pride
Faims n-glycoproteomics 
ProteomicsSystematic Discovery and Validation of T Cell Targets Directed Against Oncogenic KRAS Mutations
Jaewon Choi, Scott P. Goulding, Brandon P. Conn, Christopher D. McGann, Jared L. Dietze, Jessica Kohler, Divya Lenkala, Antoine Boudot, Daniel A. Rothenberg, Paul J. Turcott, John R. Srouji, Kendra C. Foley, Michael S. Rooney, Marit M. van Buuren, Richard B. Gaynor, Jennifer G. Abelin, Terri A. Addo...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2021-08-13 | MSV000087987 | MassIVE
ProteomicsMiProChip A microfluidic device for high-throughput single-cell proteomics via multiplexed isobaric labeling
which is character 311: The MiProChip project presents a microfluidic platform for high-throughput single-cell proteomics, integrating on-chip lysis, digestion, and TMT-based labeling to achieve efficient and low-contamination proteome processing. This dataset includes (1) manually processed single-...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) Mus Musculus (ncbitaxon:10090) 
2025-11-05 | MSV000099763 | MassIVE
ProteomicsAn Internal Standard for Assessing Phosphopeptide Recovery from Metal Ion/Oxide Enrichment Strategies
Phosphorylation-mediated signaling pathways have major implications in cellular regulation and disease. Mass spectrometry-based proteomics is a well-established approach for quantifying thousands of phosphorylation events in a single experiment. However, proteins with roles in signaling pathways are...
ORGANISM(S): Homo sapiens (Human) Saccharomyces cerevisiae (Baker's yeast) 
2018-12-04 | PXD008966 | Pride
Tmt super-heavy Lumos Phosphorylation Mtraq Tmtzero Tmtsh Tmt0 
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